Ruiz Demoulin, S. | bioRxiv Science

bioRxiv2025

Three-Dimensional-Mapping of Smooth Muscle Morphogenesis in the Vertebrate Gastrointestinal Tract

Gastrointestinal (GI) motility depends on smooth muscle contractions to propel contents through the digestive tract. GI muscle is organized into two perpendicular layers: the inner circular smooth muscle (CSM) and the outer longitudinal smooth muscle (LSM) layers. This organization is important for proper motility. Despite several investigations, GI smooth muscle development remains poorly understood due to several limitations. Previous studies mainly relied on SMA detection in tissue sections and focused on a single organ or developmental stage. They often overlooked key smooth muscle markers such as CALPONIN and {gamma}SMA and largely neglected the LSM layer. In this study, we combined confocal imaging of whole-mount three-dimensional-cleared embryonic chick guts with heatmap analysis to generate the first spatial atlas of smooth muscle development. We characterize the expression patterns of SMA, {gamma}SMA, and CALPONIN1, thereby mapping the dynamics of the formation and differentiation of CSM and LSM layers along the antero-posterior axis. This study provides a framework for future investigations into the regulatory mechanisms governing smooth muscle patterning and maturation along the GI tract.

developmental biology↗

bioRxiv2022

Neuron secreted chemokine-like Orion is involved in the transformation of glial cells into phagocytes in different neuronal remodeling paradigms

During animal development, neurons often form exuberant or incorrect axons and dendrites at early stages, followed by the refinement of neuronal circuits at late stages. Neural circuit refinement leads to the production of large amounts of neuronal debris in the form of neuronal cell corpses, fragmented axons and dendrites, and pruned synapses requiring disposal. In particular, the predominant phagocytes acting during the neuronal remodeling and degeneration are glial cells and critical signaling pathways between neurons and glia leading to phagocytosis are required. Chemokine-like mushroom body neuron secreted Orion ligand was shown to be essential to the astrocyte infiltration into the {gamma} axon bundle leading to {gamma} axon pruning and clearance of debris left from axon fragmentation. Here we show a role of orion also in debris engulfment and phagocytosis. Interestingly, we show that orion is also involved in the overall transformation of astrocytes into phagocytes. In addition, analysis of several neuronal paradigms demonstrates the role of orion in the elimination of both peptidergic vCrz+ and PDF-Tri neurons via additional phagocytic glial cells as cortex and/or ensheathing glia. Our results suggest that Orion is essential for phagocytic activation of three different types of glial cells: astrocytes, cortex and ensheathing glia and point to Orion as a trigger not only of glial infiltration but also engulfment and phagocytosis.

neuroscience↗