Rozo-Lopez, P. | bioRxiv Science

bioRxiv2024

Draft genome sequence of the glasshouse-potato aphid Aulacorthum solani

AO_SCPLOWBSTRACTC_SCPLOWAulacorthum solani is a worldwide agricultural pest aphid capable of feeding on a wide range of host plants. This insect causes mechanical damage to crops and is a vector of plant viruses. We found that the publicly available genome for A. solani is contaminated with another aphid species, and we produced a new genome using a barcoded isogenic laboratory line. We produced Oxford Nanopore and Illumina reads to generate a draft assembly, and we sequenced RNA to aid in the annotation of our assembly. Our A. solani draft genome is 671 Mbp containing 7,020 contigs with an N50 length of 196 kb with a BUSCO completeness of 98.6%. Out of the 24,981 genes predicted by E-GAPx, 22,804 were annotated with putative functions based on homology to other aphid species. This genome will provide a useful resource for the community of researchers studying aphids from agricultural and genomic perspectives.

genomics↗

bioRxiv2023

Untangling an insect virome from its endogenous viral elements

AO_SCPLOWBSTRACTC_SCPLOWInsects are an important reservoir of viral biodiversity, but the vast majority of viruses associated with insects have not been discovered. Recent studies have therefore employed high-throughput sequencing of RNA, which has led to rapid advances in our understanding of insect viral diversity. However, insect genomes frequently contain transcribed endogenous viral elements with significant homology to exogenous viruses, complicating the use of RNAseq for viral discovery. In this study, we use a multi-pronged sequencing approach to study the virome of an important agricultural pest and prolific vector of plant pathogens, the potato aphid Macrosiphum euphorbiae. We first used rRNA-reduced RNAseq to characterize the bacteria and viruses found in individual insects. We then characterized the frequency of a heritable Flavivirus and an Ambidensovirus in our population. We next generated a quality draft genome assembly for M. euphorbiae using Illumina-corrected Nanopore sequencing. This analysis showed that the Ambidensovirus, previously described from an RNAseq viral screen, is not a exogenous virus and instead is a transcribed endogenous viral element in the M. euphorbiae genome. Our study generates key insight into an important agricultural pest and highlights a widespread challenge for the study of viral diversity using RNAseq.

genomics↗