Biology subjects

Roux, M. E.

Publications and source records attributed to Roux, M. E..

3 recordsLinked to original sources

bioRxiv2022

mRNA decapping machinery targets LBD3/ASL9 transcripts to allow developmental changes in Arabidopsis

Multicellular organisms perceive and transduce multiple cues to optimize development. Key transcription factors drive developmental changes, but RNA processing also contributes to tissue development. Here, we report that multiple decapping deficient mutants share developmental defects in apical hook, primary and lateral root growth. More specifically, LATERAL ORGAN BOUNDARIES DOMAIN 3 (LBD3)/ASYMMETRIC LEAVES 2-LIKE 9 (ASL9) transcripts accumulate in decapping deficient plants and can be found in complexes with decapping components. Accumulation of ASL9 inhibits apical hook, primary root growth and lateral root formation. Interestingly, exogenous auxin application restores lateral roots formation in both ASL9 over-expressors and mRNA decay-deficient mutants. Likewise, mutations in the cytokinin transcription factors type-B ARABIDOPSIS RESPONSE REGULATORS (B-ARRs) ARR10 and ARR12 restore the developmental defects caused by over-accumulation of capped ASL9 transcript upon ASL9 overexpression. Most importantly, loss-of-function of asl9 partially restores apical hook and lateral root formation in decapping deficient mutants. Thus, the mRNA decay machinery directly targets ASL9 transcripts for decay, possibly to interfere with cytokinin/auxin responses, during development.

developmental biology↗

bioRxiv2022

PAT mRNA decapping factors function specifically and redundantly during development in Arabidopsis

Evolutionarily conserved PAT1 proteins activate mRNA decay through binding mRNA and recruiting decapping enzymes and other factors hence optimize transcriptional reprogramming during development. Here, we generated multiple mutants of pat1 (Protein Associated with Topoisomerase II), path1 and path2 and inspected their growth and leaf morphology phenotype. pat triple mutants exhibit extreme stunted growth and all mutants with pat1 exhibit leaf serration while mutants with pat1 and path1 all display short petioles. All 3 PATs can be found localized to Prossessing Bodies (PBs) upon auxin treatment and RNA-seq analysis indicate that all 3 PATs redundantly regulate auxin responses. Moreover, shade avoidance and NAC genes are misregulated in pat1path1 double and pat triple mutants suggesting PAT1 and PATH1 function in petiole elongation and leaf patterning. In conclusion, PAT proteins exhibit both specific and overlapping functions during different stages of plant growth and our observations underpin the importance of the mRNA decay machinery for proper development.

plant biology↗

bioRxiv2019

mRNA decapping machinery targets transcripts of the LBD3/ASL9 transcription factor to authorize formation of apical hook and lateral roots in Arabidopsis

Multicellular organisms perceive and transduce multiple cues to optimize developmental reprogramming and cell state switching. Key transcription factors drive developmental changes, but transitions also require the attenuation of previous states. Here, we demonstrate that the mRNA levels of the LATERAL ORGAN BOUNDARIES DOMAIN 3 (LBD3) / ASYMMETRIC LEAVES 2-LIKE 9 (ASL9) transcription factor are directly regulated by mRNA decapping. Capped ASL9 transcripts accumulate in decapping deficient plants and ASL9 mRNAs are found together with decapping components. Accumulation of ASL9 inhibits apical hook and lateral roots formation and interestingly, exogenous auxin application restores lateral roots formation in both ASL9 overexpressor and mRNA decay-deficient mutants. Moreover, mutations in the cytokinin transcription factors type-B ARABIDOPSIS RESPONSE REGULATORS (B-ARRs) ARR10 and ARR12 restore the developmental defects in apical hooking and lateral root formation caused by over-accumulation of capped ASL9 transcript upon ASL9 overexpression. Thus, the mRNA decay machinery directly targets ASL9 transcripts for decay to balance cytokinin/auxin responses during developmental reprogramming.

plant biology↗