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Rider, C. F.

Publications and source records attributed to Rider, C. F..

2 recordsLinked to original sources

Characterization of Woodsmoke Generated in the Air Pollution Exposure Lab and Comparison to Diesel Exhaust

To address the increasing concern of woodsmoke (WS) and better understand its effects on human health, a woodsmoke generation system was built in the Air Pollution Exposure Laboratory to facilitate future controlled human exposure studies. Two different woodsmoke conditions, flaming (WSFL) and smoldering (WSSM), were generated, and PM2.5 concentrations of approximately 500 g/m3 were achieved. The woodsmoke produced using the system was characterized in this study and compared with diesel exhaust (DE) generated and collected at the same facility. Within the gas phase generated by the pollutants, WS showed slight increases in CO and CO2 compared to filtered air (FA), while DE contained significantly higher levels of NOx, CO2, and total volatile organic compounds compared to FA. The WS aerosols were comprised of approximately 98% organics, 0.6% NH4, 0.9% NO3, and 0.2% SO4. Among the organic species, the CHO1 and CHOgt1 families encompassed around 60%, which was higher than the fraction of oxygenated families in DE aerosols. Moreover, the WS aerosols had higher concentrations of Cd compared to the DE aerosols. Greater oxidative potentials were also observed for WSFL and WSSM compared to DE, with DTT consumption rates normalized to the PM mass being 0.0099 and 0.0090 nmol/min/g, respectively. The difference in the compositions and properties of WS and DE suggests that it is critical to conduct further studies on how these pollutants can affect health differently.

pharmacology and toxicology↗

Woodsmoke and Diesel Exhaust: Distinct Transcriptomic Profiles in the Human Airway Epithelium

Climate change is increasing the frequency and severity of wildfires globally, causing significant woodsmoke (WS) emissions. Vehicles emit sizable amounts of toxic traffic-related air pollution (TRAP), for which diesel exhaust (DE) is a model. Both WS and DE contain particulate matter <2.5 microns (PM2.5) which deeply penetrates the lungs, causing respiratory epithelial inflammation that drives health effects. Regulations focus on PM2.5 concentration, despite emerging research that highlights how composition mediates health effects. As WS and DE are compositionally distinct, we conducted the first head-to-head comparison of effects on the transcriptomes of air-liquid interface cultured primary human bronchial epithelial cells (HBEC). Differentiated donor-matched HBEC transwells were exposed for 2-hours to filtered air (FA; control), or WS (furnace tube burning pine) or DE (Hatz 1B30E generator) both diluted to 300 {micro}g/m3 of PM2.5. WS had higher ultrafine PM, whereas DE exposure contained significantly higher NO2, CO, and O3. RNA sequencing showed that WS exposure resulted in 119 ({uparrow}41, {downarrow}78) differentially expressed genes, while DE modulated 399 ({uparrow}255, {downarrow}144) compared to FA exposure. WS was associated with small ribosomal subunit and cytochrome complex related genes, while DE exposure was associated with HIF-1 signaling, respiratory chain complex and interferon alpha/beta signaling/ISG15-protein conjugation, suggesting how TRAP exposure may enhance infection risk. We also analyzed exposure effects on protein immune-mediators. We demonstrate that two major air pollution sources modulate different genes and pathways in HBECs, with minimal overlap. This informs the debate regarding the regulatory focus on concentration and assumptions that similar concentrations of air pollution have indistinct effects. HighlightsO_LILung health effects of diesel exhaust (DE) and wood smoke (WS) are underexplored C_LIO_LIRNAseq of DE and WS-exposed primary lung epithelial cells revealed larger DE effects C_LIO_LIDE showed greater repression of host antiviral response-associated genes than WS C_LIO_LIA regulatory focus on PM concentration may miss composition-specific lung effects C_LI

bioinformatics↗