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Reyes-Flores, L.

Publications and source records attributed to Reyes-Flores, L..

2 recordsLinked to original sources

Tracing the illicit Peruvian delicacy: proof-of-concept multiplex-PCR for the simultaneous detection of seven commercial Macrobrachium prawn species

The genus Macrobrachium (Decapoda, Palaemonidae) comprises nearly 300 prawn species distributed worldwide. At least nine of these species naturally inhabit the Pacific slope river systems of Peru, where most sustain significant artisanal fisheries and are highly prized as a luxury delicacy in the nations traditional cuisine. Here, we developed and validated a multiplex PCR assay with species-specific primers to identify seven Macrobrachium species of economic importance to Peru. Through robust validation, our assays achieved high specificity and efficiency, enabling fast and simultaneous identification of the seven target species. The utility of our novel multiplex PCR assay was evaluated as a proof-of-concept tool for conducting the first molecular monitoring of species diversity and illegal trade in Peruvian prawns across different commercial sectors. Among the 51 analyzed commercial prawn samples, a total of four species were detected, including M. caementarius (82.4%), M. rosenbergii (11.8%), M. americanum (3.9%), and M. gallus (1.9%). Notably, our molecular monitoring in restaurants revealed the first evidence of illegal trade in native wild-caught prawns in La Libertad region occurring during the reproductive closed season, when only the market of the exotic farmed prawn M. rosenbergii is permitted. These findings underscore the urgent need for enhanced monitoring programs in prawn fisheries in northern Peru, as current efforts are mainly focused on more productive central and southern regions. Our multiplex PCR assay provides a robust and efficient tool that can assist authorities and researchers in monitoring efforts, combating illegal trade, conducting diversity research, and supporting conservation.

genetics↗

Development and validation of versatile species-specific primer assays for eDNA monitoring and authentication of 10 commercially important Peruvian marine species

Molecular identification assays provide crucial support in the research and regulation of aquatic resources. Among them, species-specific primers provide strong discriminatory power for fast and simultaneous differentiation between closely related species. In this study, we used interspecific variations detected in two mitochondrial genes to develop species-specific primers for eDNA monitoring and identifying 10 fish and shellfish species commercially available within the Peruvian seafood sector. To ensure versatility and high specificity, our primers were subjected to PCR, qPCR, and sequencing methods, coupled with robust validation assays that included a) an in-silico stage using self-generated and public DNA sequences, b) an in-vitro stage using target species belonging to vouchered specimens, fresh and cooked commercial samples, early life stages, and broad taxa of non-target species, and c) an in-situ stage using eDNA samples from different Peruvian marine ecosystems. Our novel species-specific primers successfully passed the validation process with high efficiency and specificity in unequivocally identifying all target species with 100% accuracy and without cross-species amplifications, thereby making them valuable tools for eDNA monitoring, seafood authentication, and to identify and combat illegal, unreported and unregulated fishing. The identification assays presented herein can be used to support effective fishery management and conservation efforts not only in the Peruvian fishery sector but also in other countries where our target species also occur or are available as imported commodities.

genetics↗