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Reider Apel, A.

Publications and source records attributed to Reider Apel, A..

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GROQ-seq Datasets Across Transcription Factors (LacI, RamR, VanR), T7 RNA Polymerase and TEV Protease

Predicting any proteins function from its sequence alone would be a significant breakthrough in molecular biology. Although machine learning approaches have sought to tackle this, their limited generalizability reflects the absence of sufficiently large, open, diverse, and unified datasets. To address this data gap, we developed a high-throughput experimental platform called GROQ-seq (Growth-based Quantitative Sequencing). In GROQ-seq, a proteins function can be linked to a sequencing-based readout that enables scalable characterization of large variant libraries in Escherichia coli. Here, we present pilot datasets demonstrating its performance across three distinct protein function classes: transcription factors, polymerases, and proteases. The objective of this report is to present the datasets and to provide users with a clear and transparent characterization of their properties, including both the strengths and limitations.

bioengineering↗

GROQ-seq Enables Cross-site Reproducibility for High-Throughput Measurement of Protein Function

High-throughput functional assays are increasingly used to generate large-scale protein function datasets for protein engineering and machine learning applications. However, the utility of such datasets depends on the reproducibility of the underlying measurements. Here we report reproducible, quantitative measurements of protein sequence-to-function data at scale across two facilities. We analyze GROQ-seq (Growth-based Quantitative Sequencing) measurements of three bacterial transcription factors. Independent barcode measurements of the same sequence produce highly consistent functional estimates, demonstrating strong biological reproducibility (across all transcription factors the mean Root Mean Square Deviation [RMSD] {approx} 0.53 and mean Spearman {approx} 0.63). We also compared experiments performed at two facilities using a shared protocol, but with differing levels of automation and system integration. We observe strong agreement between measurements taken at the two sites (mean RMSD {approx} 0.41 and mean Spearman {approx} 0.730). Orthogonal tests further support this agreement: a classifier trained to distinguish data by site performs near random (AUC = 0.559), and top-ranking variants show strong statistical overlap between experiments. Together, these results demonstrate that GROQ-seq enables reproducible, scalable measurement of protein function suitable for large aggregated datasets.

bioengineering↗