Biology subjects

Ramirez-Castrillon, M.

Publications and source records attributed to Ramirez-Castrillon, M..

2 recordsLinked to original sources

bioRxiv2020

Papiliotrema maritimi f.a. sp. nov., a new tremellaceous yeast species associated to macrophytes in a Marshland of South Brazil

One new yeast species, Papiliotrema maritimi sp. nov., is being proposed to be suitable into the Rhynchogastremataceae family, belonging to the Tremellales clade. This new species is related to six others from the Papiliotrema genus: P. taeanensis, P. siamense, P. perniciosus, P. nemorosus, P. bandonii, P. japonica and P. fuscus. The novel species is proposed based on the phylogenetic species concept through analysis of the D1/D2 region, part of the large subunit (LSU) rRNA gene and the internal transcribed spacer (ITS) region. A total of 11 strains of Papiliotrema maritimi sp. nov. were obtained from macrophytes leaves collected in south Brazil. Papiliotrema maritimi sp. nov. differs by 12, 15, 25, 25, 25 and 29 substitutions in the D1/D2 domain from the related species P. fuscus, P. japonica, P. siamense, P. nemorosus, P. bandonii, and P. perniciosus, respectively. Concerning the ITS region, there are 11 substitutions and 52 or more substitutions when compared to P. teanensis and its closest relatives. The type strain of Papiliotrema maritimi sp. nov. is UFMG-CM-Y6048. The MycoBank number for Papiliotrema maritimi sp. nov. is MB 835603.

microbiology↗

bioRxiv2020

Effects of cellular stress on pigment production in Rhodotorula mucilaginosa/alborubescens AJB01 strain from the Caribbean region of Colombia

Considering that a large proportion of industrially used pigments are toxic and potentially mutagenic, with some having carcinogenic effects, it is essential to replace them as well as eliminate them from industrial effluents. The discovery of biopigments has received considerable attention from the industry sector. The main objective of this study was to increase the production of carotenoid pigments in naturally pigmented yeast by subjecting the yeast to various cellular stresses. The fungal strain Rhodotorula mucilaginosa/alborubescens AJB01 was isolated from a food sample collected in Barranquilla, Colombia, and one of the pigments produced was {beta}-carotene. This strain was subjected to various stress conditions, including osmotic stress using different salts, physical stress by ultraviolet (UV) light, and light stress using different photoperiods. The optimal growth conditions for carotenoid production were determined to be 1 min of UV light, 0.5 mg/L of magnesium sulfate, and 18:6 h light/dark period, which resulted in a carotenoid yield of 118.3 g of carotenoid per gram of yeast.

microbiology↗