Biology subjects

Ramirez, S.

Publications and source records attributed to Ramirez, S..

39 records · Page 3Linked to original sources

bioRxiv2019

Comparative of probiotics reveals cecal microbial component associated with performance parameters in broilers

Probiotics have become increasingly popular in poultry industry as a promising nutritional intervention to promote modulation of intestinal microbiota as a means of improving health and performance. This study aimed to determine the effects of different probiotic formulations on the cecal microbial communities and performance in 21 and 42 day-old-broilers, as well as to define associations between ceca microbial profile and growth parameters. Probiotics investigated included a synbiotic (SYNBIO), a yeast-based probiotic (YEAST), and three single-strain formulations of spore-forming Bacillus amyloliquefaciens (SINGLE1), B. subtilis (SINGLE2) and B. licheniformis (SINGLE3). Dietary inclusion of SYNBIO, YEAST, and SINGLE2 increased body weight (BW) by 7, 14, and 21d (p<0.05) compared to a basal diet without probiotics (CON). The treatments SYNBIO, and YEAST decreased mortality by 21d, while SYNBIO reduced the overall mortality rate by 42d (p<0.05). Bifidobacteriales had the highest (p<0.05) population in SINGLE2, whereas Clostridiales was reduced compared to CON, SINGLE1, and SINGLE3. The addition of SYNBIO into diet mainly stimulated (p<0.05) the cecal relative abundance of Lactobacillales by 21d. Besides, Spearmans correlation analyses revealed that population of Lactobacillales was associated with lower Enterobacteriales, higher BW, and lower mortality of growing broilers. These results suggest that the modulation of ceca microbiota and the greatest productive parameters were achieved by supplementation of specific probiotic mixture. The selection of probiotics by their ability to drive cecal microbiota towards lactic acid bacteria colonization may be a strategic approach to improve the indicators of performance in broilers.

microbiology↗

bioRxiv2019

Comparative effectiveness of probiotic-based formulations on cecal microbiota modulation in broilers

The potential of probiotics to manipulate the intestinal microbial ecosystem toward commensal bacteria growth offers great hope for enhancing health and performance in poultry. This study aimed to evaluate the efficacy of five probiotic-based formulations in modulating cecal microbiota in broilers at 21 and 42 days of age. Probiotics investigated included a synbiotic (SYNBIO), a yeast-based probiotic (YEAST), and three single-strain formulations of spore-forming Bacillus amyloliquefaciens (SINGLE1), B. subtilis (SINGLE2) and B. licheniformis (SINGLE3). Metagenomics analyses showed that the cecal microbiota of SINGLE, YEAST, and MULTI supplemented birds had low diversity compared to the control diet without probiotics (CON) at 21d. At the same age, birds fed SYNBIO had a high population of Lactobacillus salivarus and Lachnospiraceae, which was reflected in a singular microbial community illustrated by beta-diversity analyses. By 42d, there were no differences in alpha or beta-diversity in the microbiota of probiotic-treated broilers compared to CON. Birds treated with SINGLE2 had a lower abundance of lactic acid bacteria, while a higher (p<0.05) population of unidentified Lachnospiraceae and Ruminococcaceae. Although the investigated probiotic formulations shared similar taxonomy, modulation of microbiota was not a core outcome for all probiotic-treated broilers indicating the success of microbiota-based intervention may ultimately be dependent on probiotic mixture and broilers age.

microbiology↗

bioRxiv2017

The Nuclear And Mitochondrial Genomes Of The Facultatively Eusocial Orchid Bee Euglossa dilemma

Bees provide indispensable pollination services to both agricultural crops and wild plant populations, and several species of bees have become important models for the study of learning and memory, plant-insect interactions and social behavior. Orchid bees (Apidae: Euglossini) are especially important to the fields of pollination ecology, evolution, and species conservation. Here we report the nuclear and mitochondrial genome sequences of the orchid bee Euglossa dilemma Bembe & Eltz. Euglossa dilemma was selected because it is widely distributed, highly abundant, and it was recently naturalized in the southeastern United States. We provide a high-quality assembly of the 3.3 giga-base genome, and an official gene set of 15,904 gene annotations. We find high conservation of gene synteny with the honey bee throughout 80 million years of divergence time. This genomic resource represents the first draft genome of the orchid bee genus Euglossa, and the first draft orchid bee mitochondrial genome, thus representing a valuable resource to the research community.

genomics↗