Biology subjects

Radwan, J.

Publications and source records attributed to Radwan, J..

14 recordsLinked to original sources

bioRxiv2026

Does Borrelia afzelii outer surface protein E coevolve with complement factor H of its rodent host? Insights from GxG and spatial associations

BackgroundLyme borreliosis is a common tick-borne disease in Europe caused by spirochetes of the Borrelia burgdorferi sensu lato complex, including Borrelia afzelii, which is maintained in nature through interactions with rodent reservoir hosts. These spirochetes have evolved several surface proteins to manipulate rodent host immunity, some of which remain polymorphic in Borrelia populations. Among these proteins, OspE, which binds the host complement-regulating factor CFH to evade destruction by complement, is one of the most variable. Yet, what evolutionary forces maintain this polymorphism is not well understood. Motivated by a recent discovery of CFH polymorphism in the bank vole (Clethrionomys glareolus), the main reservoir host of B. afzelii, we hypothesized that the polymorphism is maintained by host-parasite coevolution involving specific associations between host and parasite genetic variants. MethodsWe analyzed associations between bank vole CFH alleles and B. afzelii OspE variants across three datasets sampled in Poland. Selection acting on OspE was evaluated using omegaMap. Host-pathogen genotype associations were tested using partial redundancy analysis (RDA), and co-structure was assessed using co-correspondence analysis (CoCA). ResultsWe found that OspE evolves under positive selection, however, we found no evidence for an association between OspE and host CFH variants at the individual level based on RDA or at the population level based on CoCA. ConclusionsDespite evidence of positive selection acting on OspE, we found no support for specific genetic matching between B. afzelii and its bank vole host at the CFH-OspE interface. These results suggest that the evolution of CFH and OspE may be shaped by broader selective pressures, potentially including interactions with multiple host species.

evolutionary biology↗

bioRxiv2026

Population Resilience Under Environmental Deterioration in Socially Monogamous Systems with Mutual Mate Choice

Rapid environmental change and biodiversity loss make it increasingly important to identify factors influencing population extinction risk. Previous studies examining how mating systems can affect persistence of populations under environmental stress generally report higher extinction risks in monogamous than polygynous systems but have largely ignored extra-pair copulations (EPC) and paternity (EPP), despite the prevalence of genetic polyandry in socially monogamous species. Here, using an individual-based model, we study how EPP in socially monogamous systems affects population resilience under directional environmental change. We assume that in socially monogamous species, both sexes carry costly sexual ornaments, the elaboration of which depends on the strength of preference. The effect of EPPs on extinction risk depended on the strength of mate preference, population size, and the degree to which homozygosity affected fitness. Systems with EPCs are not simply intermediate in resilience between strict monogamy and polygyny: the preference strength interacts with mating system, leading to superior resilience of EPC systems compared to strictly monogamous and polygynous systems when choosiness and the negative consequences of heterozygosity loss are low, and EPP rates are high. However, this benefit was reduced in small populations due to faster loss of heterozygosity. At high choosiness, EPC systems exhibited lower resilience than socially polygynous choice systems because the higher reproductive skew of the latter system allowed them to adapt faster while not suffering from the demographic consequences of sexual signaling costs borne by females. Overall, our results suggest that EPCs can enhance population resilience when females obtain fertilizations from higher-condition extra-pair males compared to systems without EPC.

evolutionary biology↗

bioRxiv2026

Mating Systems and Evolutionary Rescue

Animal mating systems are hugely diverse, ranging from species where mating is essentially random to those exhibiting complex systems of mate choice by one or both sexes, with some species mating monogamously and others showing various degrees of promiscuity. There is now good evidence that if male signal traits are correlated with fitness, then polygynous female choice systems can show improved adaptation and persistence, but there has been little exploration of the ways that other types of mating systems modulate adaptation and evolutionary rescue. To address this, we developed an individual-based model that allows random mating, female-only choice, and mutual mate choice to be compared within both monogamous and polygynous frameworks and used it to explore how mating systems influence adaptive response, loss of heterozygosity, and extinction risk under changing environmental conditions. We find that mating system interacts with population size in determining extinction risk: because mate choice under polygyny lowers effective population size, accelerating the loss of heterozygosity, small polygynous populations with either mutual or female-only mate choice face higher extinction risks than randomly mating populations. In larger populations where inbreeding and genetic drift are less important, female-choice and mutual-choice polygynous systems show the greatest resilience to environmental change by allowing better-adapted males to dominate reproduction. Random mating populations show the lowest resilience to environmental change when populations are large and mutual-choice monogamous systems have intermediate resilience. Among polygynous systems, female-only choice leads to slower loss of heterozygosity and facilitates population resilience better than mutual mate choice. These findings demonstrate that mating systems can critically shape a populations ability to adapt to environmental change and alter extinction risks, emphasizing the need to consider mating systems in designing effective conservation strategies.

evolutionary biology↗

bioRxiv2026

Experimental evolution under biased sex ratios: phenotypic and genomic responses in the bulb mite, Rhizoglyphus robini

Sexual selection may increase population fitness by favouring high-condition individuals and accelerating the purging of deleterious alleles. However, it can also reduce population fitness through intra- and interlocus sexual conflict by promoting male-benefit traits that harm females and maintain polymorphism at sexually antagonistic loci. The balance between these opposing forces remains unresolved, yet it has major consequences for how sexual selection shapes population fitness and genome-wide variation. To explore the genomic and phenotypic effects of sexual selection and sexual conflict, we evolved replicated bulb mite (Rhizoglyphus robini) lines for 28 generations under male- versus female-biased sex ratios and combined phenotypic assays with whole-genome resequencing. Female fecundity and inbreeding depression did not differ between treatments, and genomic analyses revealed no treatment effect on the loss of rare, putatively deleterious SNPs. Contrary to expectations, males from male-biased lines were less harmful to stock females than males from female-biased lines. Genome-wide nucleotide diversity declined similarly across generations in both treatments, although synonymous exonic diversity declined more slowly in male-biased lines. While only a few SNPs diverged consistently between treatments, we identified large treatment-specific haplotype blocks indicating that multiple genomic regions were involved in response to sex-ratio manipulation. Overall, our results indicate that sex ratio manipulation drives evolution of male harm to females and widespread haplotype frequency changes without clear evidence for enhanced purging or maintenance of genetic diversity. The response thus appears to reflect adaptation to altered level of reproductive competition, but without measurable consequences for population fitness and genetic diversity. Significance statementSexual selection is often proposed to improve population fitness by removing deleterious mutations, yet it can also favour traits that harm the opposite sex; consequently, it remains unclear whether stronger reproductive competition reliably enhances population viability. By evolving bulb mite populations under strongly male- or female-biased sex ratios, we found that male-biased populations did not purge genetic load more effectively, while the genomic response to sex-ratio bias was highly polygenic. In contrast to our predictions, males from male-biased lines were less harmful to females than males from female-biased lines. Overall, our results show that sex-ratio bias can reshape male phenotypes and generate patterns of genomic divergence, but without any significant effect on population fitness. O_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=152 SRC="FIGDIR/small/701246v1_ufig1.gif" ALT="Figure 1"> View larger version (61K): org.highwire.dtl.DTLVardef@15afcf7org.highwire.dtl.DTLVardef@95ef16org.highwire.dtl.DTLVardef@183c405org.highwire.dtl.DTLVardef@1ba3159_HPS_FORMAT_FIGEXP M_FIG C_FIG

genomics↗

bioRxiv2026

Genomic basis of adaptation to constant and fluctuating environments in a global pest of cereals

In agricultural systems, spatially and temporally heterogeneous environments are expected to favour the evolution of generalist pests and pathogens, yet the genomic mechanisms underlying niche-breadth expansion remain poorly understood. Here, we address this gap by assembling one of the smallest known eukaryotic genomes--from the global cereal pest Aceria tosichella--and by resequencing generalist and specialist populations which evolved in the lab on constant versus fluctuating hosts. A previous study demonstrated that generalist populations retained a wider niche, including the ability to exploit a refuge host, compared to specialist populations that showed a narrowing of the ecological niche. Genomic scans identified 640 SNPs that significantly differed in frequency between treatments, including a single highly differentiated 120-kbp region containing 13 genes. Allele-frequency shifts across many SNPs in this region paralleled phenotypic divergence in the ability to utilise refuge hosts, and gene annotations suggested their roles in starvation resistance and nutrient signalling. However, experimental validation of the top candidate gene did not support its strong direct effect on survival on the refuge host, implying a more complex, likely polygenic basis for adaptation. Consistent with this interpretation, numerous significant SNPs occurred outside the focal region, with enrichment for genes involved in xenobiotic transport, detoxification pathways, and ABC transporters. Together, these results indicate that generalism in A. tosichella relies on complex molecular mechanisms that enable survival on suboptimal or refuge hosts, relying to a considerable extent on the ability to deal with stress arising, for example, from toxic compounds present in suboptimal hosts.

evolutionary biology↗

bioRxiv2025

A key regulator of missing-self innate immunity is polymorphic and under diversifying selection

Host-parasite co-evolution drives the diversification of host immune genes involved in the recognition of pathogen antigens and molecular patterns. In contrast, the immune genes involved in self-recognition and inhibition of immune responses against self-cells (missing-self immunity) are expected to be evolutionarily constrained. However, many pathogens, such as the Lyme disease agent Borrelia, hijack these genes to evade the immune system and may therefore select for their diversification. How these contrasting but concurrent selective forces shape the evolution of missing-self regulators is not clearly understood. To fill this gap, we investigated polymorphism and molecular signatures of selection acting on a missing-self regulator, the Complement Factor H (CFH), in bank vole populations, which are an important wild reservoir for Borrelia. We then compared the geographic structuring in the CFH domain interacting with Borrelia (CCP 20) against a genomic background represented by RAD-seq markers. We found signals of positive and diversifying selection at CCP 20, suggesting that CFH evolved in response to pressures from pathogens. Additionally, we found other innate immunity genes within the alternative complement pathway, which is regulated by CFH, under diversifying selection, highlighting its involvement in host-parasite coevolution. This study demonstrates that an innate missing-self sensor in a wild vertebrate is under diversifying selection, likely driven by pathogens.

evolutionary biology↗

bioRxiv2025

Immune Signatures of Gastrointestinal Helminth Infection in Transcriptomes of European Bison

Understanding the genes and molecular pathways that shape host responses to infection is essential for advancing our knowledge of host-parasite interactions and their ecological and evolutionary implications. Such insights are especially valuable for conserving endangered species that may be vulnerable to emerging or novel pathogens. Here, we investigated the impact of the recently introduced gastrointestinal nematode Ashworthius sidemi on gene expression in its novel host, the European bison (Bison bonasus). We analyzed abomasal transcriptomes (n = 45) from individuals characterized by a wide range of infection intensities (0-8,620 parasites per host). Despite substantial variation in parasite burden, differential expression analyses detected no individual genes significantly associated with infection intensity. However, gene set enrichment analyses based on p-value distributions revealed multiple immune-related gene ontology categories, including B and T cell activation, neutrophil chemotaxis, inflammatory responses, and regulation of IL-6 production. These findings indicate that European bison mount a clear yet subtle transcriptional response to A. sidemi infection and highlight molecular pathways potentially involved in mediating host defense against this emerging parasite.

immunology↗

bioRxiv2025

Interplay between purging and admixture shapes genetic load in an invasive guppy population

Demographic history can shape the genetic load of populations by influencing the efficacy of selection, levels of heterozygosity, and the incorporation of new variants via gene flow. Understanding these dynamics is crucial for identifying threats to population viability and predicting evolutionary trajectories of invasive populations translocated by humans into non-native environments. We investigate these processes in Trinidadian guppies (Poecilia reticulata) by estimating genetic loads across multiple populations, with a particular focus on a single expanding population in which translocated individuals have rapidly spread and replaced native individuals. Overall, we observe the expected negative relationship between neutral genetic diversity and relative genetic load. In the translocated population, patterns differ between strongly and weakly deleterious mutations. Strongly deleterious alleles are purged at the isolated translocation site but tend to accumulate along the expansion front. In contrast, the genetic load estimated based on weakly deleterious variants declines along the expansion gradient. These differing patterns can be explained by admixture with native populations (which carried fewer weakly deleterious mutations) reducing the overall genetic load of the population at the expansion front. However, admixture has also increased genetic diversity and introduced new strongly deleterious alleles, thereby reversing the purging effect. Together, our findings illustrate the complex interactions determining genetic load in subdivided populations, offering important insights into the evolutionary aspects of biological invasions.

evolutionary biology↗

bioRxiv2025

Fighting to persist: Genetic rescue increases long-term fitness despite elevated genetic load

Genetic rescue (GR) can restore fitness in small genetically depauperate populations, however, long-term effectiveness, impact of rescuer quality and introduced deleterious load on population persistence remains uncertain. Using a male-dimorphic mite we tested whether a condition-dependent sexually selected trait influences GR. Genome-wide diversity and genetic load increased similarly for both morphs, while population fitness trajectories differed, suggesting that genetic quality is associated with the trait. Overall, GR sustained fitness benefits over 20 generations. The benefits persisted under thermal stress lowering extinction risk regardless of the rescuers morph and introduced load indicating their minimal importance. These results provide experimental evidence that maximising genome-wide variation via GR can yield lasting benefits, even when many deleterious mutations are introduced to populations facing environmental change.

evolutionary biology↗

bioRxiv2025

Fighting Through the Heat: How Sexual Selection Influences Demography Under Recurrent Heatwaves

Sexual selection is a potent evolutionary force with complex effects. Strong sexual selection can enhance adaptation and reduce mutational load, while simultaneously reducing survival, or causing sexual conflict that reduces fitness for one or both sexes. Many populations today face not only gradual environmental changes but also extreme, short-term stress events like droughts or heatwaves. The combined effects of sexual and environmental selection on population demography during and after such events remain poorly understood, even though such combined effects could be crucial for the persistence of small, endangered populations under climate change. In this study, we investigated how sexual selection affects survival during environmental stress by manipulating the expression of an aggressive fighter morph in small populations of the male-dimorphic soil mite Sancassania berlesei, exposing some of these populations to recurrent periods of extreme heat and monitoring survival over eight generations. We found that heat exposure reduced survival, more severely in females than in males, and survival was lower in populations with higher fighter prevalence, but there was no interaction between temperature and fighter morph prevalence. Furthermore, survival declined across generations, and the decline was steeper in populations with lower prevalence of fighters, leading to the loss of their initial survival advantage by the last generation. T265625hree populations exposed to heat became extinct during the experiment, all from the reduced fighter expression treatment. Our findings imply that despite its cost to individual survival, sexual selection does not modulate population sensitivity to heatwaves over several generations. Furthermore, we demonstrate that these mortality costs of sexual selection are gradually compensated over successive generations, which could be a result of a more effective purging of inbreeding depression. Thus, while the additive effect of sexual selection and heatwaves on survival may increase demographic risks for bottlenecked populations in the short term, sexual selection may increase resilience of populations to prolonged bottlenecks.

evolutionary biology↗

bioRxiv2024

Sex-specific recombination landscape in a species with holocentric chromosomes

The rate and chromosomal positioning of meiotic recombination significantly affects the distribution of the genetic diversity in eukaryotic genomes. Many studies have revealed sex-specific recombination patterns, with male recombination typically biased toward chromosome ends, while female recombination is more evenly distributed along chromosomes, or concentrated in pericentromeric region. It has been proposed that such patterns in females may counteract meiotic drive caused by selfish genetic elements near centromeres and should not occur in species devoid of clearly defined centromeres, but evidence for this expectation is scarce. Here, we constructed a sex-specific genetic map of a species with holocentric chromosomes, the bulb mite (Rhizoglyphus robini), a model organism for sexual selection studies with heritable alternative male reproductive phenotypes. We found a similar recombination landscape in both males and females, with a consistent pattern of increased rates towards both chromosome ends, and a higher recombination rate in females than in males. Recombination rate was positively correlated with repeat density and negatively with gene density. Our results are consistent with the meiotic drive hypothesis and suggest that the evolution of recombination patterns is closely linked to chromosome features.

genomics↗

bioRxiv2020

Expansion of frozen hybrids in guppy ectoparasite, Gyrodactylus turnbulli

Hybridization is one of the major factors contributing to the emergence of highly successful parasites. Hybrid vigor can play an important role in this process, but subsequent rounds of recombination in the hybrid population may dilute its effects. Increased fitness of hybrids can, however, be frozen by asexual reproduction. Here, we identify invasion of a "frozen hybrid" genotype in natural populations of Gyrodactylus turnbulli, a facultatively sexual ectoparasitic flatworm that causes significant damage to its fish host. We re-sequenced genomes of these parasites infecting guppies from six Trinidad and Tobago populations, and found surprisingly high discrepancy in genome-wide nucleotide diversity between islands. The elevated heterozygosity on Tobago is maintained by predominantly clonal reproduction of hybrids formed from two diverged genomes. Hybridization has been followed by spread of the hybrids across the island, implying a selective advantage compared to native genotypes. Our results thus highlight that a single outcrossing event may be independently sufficient to cause pathogen expansion.

evolutionary biology↗

bioRxiv2018

Sexual selection drives the evolution of wing interference patterns.

The seemingly transparent wings of many insects have recently been found to display dramatic structural coloration. These structural colours (wing interference patterns: WIPs) may be involved in species recognition and mate choice, yet little is known about the evolutionary processes that shape them. Additionally, existing research has been restricted by analysing WIPs without due consideration of how they are actually perceived by the viewers colour vision. Here, we use multispectral digital imaging and a model of Drosophila vision to compare WIPs of male and female Drosophila simulans from replicate populations forced to evolve with or without sexual selection for 68 generations. We show for the first time that WIPs modelled in Drosophila vision evolve in response to sexual selection, and confirm that WIPs correlate with male sexual attractiveness. These findings add a new element to the otherwise well described Drosophila courtship display and confirm that wing colours evolve through sexual selection.

evolutionary biology↗

bioRxiv2017

Comprehensive profiling of TCRβ repertoire in a non-model species (the bank vole) using high-throughput sequencing

In recent years, immune repertoire profiling with high-throughput sequencing (HTS) has advanced our understanding of adaptive immunity. However, fast progress in the field applied mostly to human and mouse research, with only few studies devoted to other model vertebrates. We present the first in-depth characterization of the TCR{beta} repertoire in a non-model mammal with limited genomic resources available - the bank vole (Myodes glareolus). We used 5'RACE and Illumina HTS to describe V and J segments and to qualitatively characterize preferential V-J segment usage and CDR3 length distribution. Finally, a molecular protocol integrating unique molecular identifiers was used for quantitative analysis of CDR3 repertoire with stringent error correction. We found 37 V and 11 J genes that were orthologous to mice genes. A conservative, lower bound estimation of the TCR{beta} repertoire was 1.7-2.3x105 clonotypes, and the degree of sharing of the observed repertoire between any two individuals was 3.6% of nucleotide sequences and 14.3% of amino acid sequences. Our work adds a crucial element to the immunogenetic resources available for the bank vole, an important species in ecological and evolutionary research. The workflow that we developed can be applied for immune repertoire sequencing of non-model species, including endangered vertebrates.

immunology↗