Biology subjects

RS, S.

Publications and source records attributed to RS, S..

3 recordsLinked to original sources

bioRxiv2022

Shelf-Life Specific Moisture Variation In Chitosan Of Genus Fenneropenaeus Distributed Along Arabian Sea, India

Chitosan is a characteristic polysaccharide, naturally extracted from the crustaceans shells. The stability and shelf-life of chitosan are affected by a few parameters, basically the moisture content. In this study, three species of shrimps such as Indian white shrimp (Fenneropenaeus indicus), banana shrimp (F. merguensis), and tiger prawn (F. monodon) were utilized for the extraction of chitosan. The extraction strategy included the method of demineralization, deproteinization, and deacetylation. Later the yield and moisture of chitosan were examined from three diverse species. The yield of shell waste ranged between 46% to 50% (on moist weight premise) and the chitosan was between 18.0 to 20.2%. Moisture content always plays a critical part in chitosan shelf life and stability and was between 5.2% to 6.8 %.

zoology↗

bioRxiv2021

Peptide-based epitope design on non-structural proteins of SARS-CoV-2

The SARS-CoV-2 virus has caused the severe pandemic, COVID19 and since then its been critical to produce a potent vaccine to prevent the quick transmission and also to avoid alarming deaths. Among all type of vaccines peptide based epitope design tend to outshine with respect to low cost production and more efficacy. Therefore, we started with obtaining the necessary protein sequences from NCBI database of SARS-CoV-2 virus and filtered with respect to antigenicity, virulency, pathogenicity and non-homologous nature with human proteome using different available online tools and servers. The promising proteins was checked for containing common B and T-cell epitopes. The structure for these proteins were modeled from I-TASSER server followed by its refinement and validation. The predicted common epitopes were mapped on modeled structures of proteins by using Pepitope server. The surface exposed epitopes were docked with the most common allele DRB1*0101 using the GalaxyPepDock server. The epitopes, ELEGIQYGRS from Leader protein (NSP1), YGPFVDRQTA from 3c-like proteinase (nsp5), DLKWARFPKS from NSP9 and YQDVNCTEVP from Surface glycoprotein (spike protein) are the epitopes which has more hydrogen bonds. Hence these four epitopes could be considered as a more promising epitopes and these epitopes can be used for future studies.

bioinformatics↗

bioRxiv2021

Ligand geometry dictates cellular and in vivo uptake of 3D DNA nanostructures

Fabrication of nanoscale DNA devices to generate 3D nano-objects with precise control of shape, size, and presentation of ligands has shown tremendous potential for therapeutic applications. The interactions between different topologies of 3D DNA nanostructures and the cell membranes are crucial for designing efficient tools for interfacing DNA devices with biological systems. The practical applications of these DNA nanocages are still limited in cellular and biological systems owing to the limited understanding of interactions of different surface topologies of DNA nanodevices with cell membranes. The correlation between the geometry of DNA nanostructures and their internalization efficiency remains elusive. We investigated the influence of the shape and size of 3D DNA nanostructure on their cellular internalization efficiency. We found that of different geometries designed, one particular geometry, i.e., the tetrahedral shape, is more favoured over other geometries for their cellular uptake in 2D and 3D cell models. This is also replicable for cellular processes like 3D cell invasion assays in 3D spheroid models and passing the epithelial barriers in in-vivo zebrafish model systems. Our work establishes ground rules for the rational designing of DNA nanodevices for their upcoming biological and biomedical applications.

bioengineering↗