Qiang, D. | bioRxiv Science

bioRxiv2025

A Method for Preparing Morphologically Preserved Wildlife Fecal Specimens for Long-Term Ecological Studies

O_LIWildlife feces are a valuable noninvasive resource in ecological and conservation research. However, traditional preservation methods are unable to maintain morphological integrity while simultaneously preserving the biological and chemical composition of fecal samples. C_LIO_LIThis study presents a novel method for the preparation of fecal specimens through a multistep immersion process using sodium carboxymethyl cellulose, sodium benzoate, clotrimazole, ethanol, pyrethroid emulsion and polyvinylpyrrolidone solution. C_LIO_LIThe specimens produced by this method exhibited high mechanical strength, ensuring durability and resistance to handling damage. During a storage period of six months, this method successfully preserved the morphological characteristics of fecal samples while maintaining DNA integrity, with no signs of mold or insect damage. DNA extraction achieved a 100% success rate and species identification remained consistent with fresh samples, with BLAST match rates exceeding 99% in 15 specimens. In addition, heavy metals such as chromium, arsenic, and lead were detected in fecal samples from different species. C_LIO_LIBy allowing long-term preservation of fecal samples, this method transforms urine from a short-term diagnostic tool into a durable resource for monitoring biodiversity. It can extend the applications of fecal samples across spatial and temporal scales, reinforcing their role in ecological research and global conservation of biodiversity. C_LI

ecology↗

bioRxiv2023

Regulation of the trade-off between cold stress and growth by BcGSTF10

Cold stress represents a significant threat to global agricultural productivity and food security. Typically, plant resistance to cold stress is accompanied by a growth deficit and a reduction in yield. In this study, we have discovered a novel gene, BcGSTF10, which has not been previously reported to be involved in low-temperature stress. On the one hand, biochemical and genetic analyses have demonstrated that BcGSTF10 interacts with BcICE1 to promote the expression of CBF genes, thereby enhancing freezing stress tolerance in non-heading Chinese cabbage [NHCC, Brassica campestris (syn. Brassica rapa) ssp. chinensis] and Arabidopsis. On the other hand, the expression of the BcGSTF10 gene is directly repressed by BcCBF2, while BcGSTF10 exhibits a positive role in growth in both NHCC and Arabidopsis. This dual function of BcGSTF10 indicates its pivotal role in balancing low-temperature stress and growth, which will inform the development of strategies to breed climate-resilient and high-yield crops, thereby contributing to sustainable agriculture.

plant biology↗