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Pudasaini, S.

Publications and source records attributed to Pudasaini, S..

2 recordsLinked to original sources

Varietal Evaluation of Coffee Genotype in Subtropical Nepal

Coffee (Coffea arabica L.) is an emerging high-value crop in Nepal, but national productivity remains far below genetic potential, partly due to the lack of locally validated variety recommendations. This study evaluated and compared the morphological and yield-attributing characteristics of 19 Arabica genotypes at the Horticulture Research Station, Malepatan, Pokhara (848 m above sea level) from January to June 2026 using a Randomized Complete Block Design with three replications. Yellow Caturra recorded the highest total fresh cherry yield (5.34 kg/plant), followed by Caturra Amarillo (3.56 kg/plant), Catuai Vermelo (3.19 kg/plant), and Catuai Amarillo (3.10 kg/plant). Catuai Amarillo and Caturra Amarillo produced the most bearing branches (76 and 72, respectively), while Caturra Amarillo and Yellow Caturra had the most cherries per node (7.73 and 7.47). Pacamara and Selection-10 achieved the maximum 100-cherry weight (167.67 g), while Indonesia had the largest cherry diameter (13.38 mm). Catuai Amarillo had the highest total soluble solids (25.97 degree Brix), while Tekisic had the lowest (16.80 degree Brix). Coffee leaf rust severity increased from a mean of 7.49% at 30 days after incidence (DAI) to 37.58% at 120 DAI. Selection-10 was the least affected genotype across the severity assessments and recorded the lowest area under the disease progress curve (AUDPC; 25.03 rating-days), whereas Caturra Amarillo had the highest AUDPC (347.95 rating-days). Overall, Yellow Caturra showed the strongest yield performance, while Selection-10 combined low disease development with a high 100-cherry weight. These results indicate that genotype selection should consider productivity, quality-related traits, and CLR response together.

plant biology↗

Smartphone-Operated Affordable PCR Thermal Cycler for the Detection of Antimicrobial Resistant Bacterial Genes

ObjectivesAntimicrobial resistance (AMR) is a global public health threat. Surveillance of AMR requires affordable, rapid, and user-friendly diagnostic method. Our aim was to develop a low-cost thermocycler to perform polymerase chain reaction (PCR). MethodsWe developed a smartphone-operated PCR thermal cycler using locally available recycled materials. The thermal cycler was used for the amplification for three bacterial genes - bla-TEM and bla-CTXM and 16s rRNA in human urine samples. The performance of custom-built thermal cycler was compared with the commercial one. ResultsThe thermal cycler was portable (<1kg weight), required 12 V power supply, 25 {micro}L of solution, and cost only USD50.0. Temperature and time conditions were instructed using a custom-built smartphone application. The ramping rate of was 0.23{degrees}C for heating and 0.43{degrees}C for cooling, set temperatures were within {+/-} 0.5 {degrees}C of target showing a good thermal stability. The antibiotic sensitivity test of human urine samples showed they were highly resistance and multi-resistant. Nearly 46 % (n=54) E. coli isolates were positive in ESBL screening test. The custom-built thermocycler was able to accurately predict the presence of bla-TEM and bla-CTXM genes (n=6). ConclusionsWe developed and demonstrated a portable, low-cost, easy-to-use, and smartphone-operated PCR thermal cycler. Since it is portable, it can be used in remote location and field settings, including places without stable power supply. The use of the thermal cycler system can be extended, beyond the detection of AMR genes, e.g., in clinical diagnosis, genetics, forensic analysis, and environmental protection.

molecular biology↗