Biology subjects

Prosekov, A. Y.

Publications and source records attributed to Prosekov, A. Y..

8 recordsLinked to original sources

bioRxiv2026

Molecular and spatial characterization of baicalin from Scutellaria baicalensis hairy root culture

This study presents the structural verification of baicalin isolated from a hydroethanolic extract of an in vitro Scutellaria baicalensis root culture using X-ray diffraction analysis and a set of NMR spectroscopy techniques. The crystalline molecular structure of the sample was found to correspond to baicalin. The 1H, 13C{1H}, 2D 1H1H-COSY, 1H13C-HSQC, 1H13C-HMBC spectra confirmed that the chemical shifts, signal multiplicities, integral intensities, and spin-spin coupling constants were fully consistent with the structure of the target compound. Minor impurity signals were detected in the aliphatic region of the spectra, with a total content not exceeding 5 mol%. These results confirm the high purity and structural individuality of baicalin, a biologically active flavonoid glycoside of considerable interest.

plant biology↗

bioRxiv2026

Structural analysis of chlorogenic acid from red clover (Trifolium pratense) extract

This paper presents the results of a structural analysis of chlorogenic acid isolated from a 70% ethanol extract of red clover (Trifolium pratense) callus culture. X-ray phase analysis showed that the sample was crystalline and single-phase and crystallized in an orthorhombic unit cell with the following parameters: a = 36.7548(5) [A], b = 11.0770(3) [A], c = 7.7947(2) [A], V = 3173.46(11) [A]3, R-Bragg = 0.347 %, Rexp = 4.75 %, Rwp = 5.83 %, Rp = 4.39 %, GOF = 1.23 %. NMR spectroscopy data (1H, 13C{1H}, 2D 1H1H-COSY, 1H13C-HSQC, 1H13C-HMBC) confirmed that the chemical structure and purity of the sample fully corresponded to chlorogenic acid, with no chemical impurities detected. Complete proton and carbon atom assignments are provided.

plant biology↗

bioRxiv2025

Study of the molecular and spatial structure of trans-cinnamic acid isolated from the hairy root culture of Scutellaria baicalensis

In this study, the crystalline structure of a sample of trans-cinnamic acid, isolated from an aqueous-alcohol extract of Scutellaria baicalensis in vitro root culture was analyzed using diffraction analysis. The diffraction analysis results confirmed that the investigated trans-cinnamic acid sample corresponds to the declared compound: trans-cinnamic acid. Additionally, the structure of this sample was examined using NMR spectroscopy. The obtained 1H, 13C{1H}, 1H1H-COSY, 1H13C-HSQC, 1H13C-HMBC NMR spectra of the studied sample correspond to the structure of the target compound, trans-cinnamic acid. No impurity signals were detected in the analyzed trans-cinnamic acid sample.

plant biology↗

bioRxiv2025

Evaluation of the antioxidant activity of biologically-active compositions based on plant metabolites produced by in vitro cell cultures

Free radicals can cause oxidative stress in the body, which, in turn, leads to various diseases such as cardiovascular diseases, diabetes, Alzheimers disease, Parkinsons disease, and others. To protect against the destructive effects of radicals, living organisms utilize antioxidants. One of the most important classes of antioxidants is polyphenols. The primary effect of polyphenols on the body is associated with their ability to capture and neutralize free radicals, as well as reactive oxygen and nitrogen species. This study focuses on investigating the antioxidant activity (AA) of polyphenols and their compositions based on rutin, quercetin, and trans-cinnamic acid, produced by in vitro cell cultures, with the aim of developing dietary supplements with geroprotective properties. The AA of the studied substances was assessed using the ABTS and DPPH free radical scavenging methods. The study established that, according to the ABTS method, AA decreases in the order of quercetin > rutin > trans-cinnamic acid, while according to the DPPH method, the order is rutin > quercetin > trans-cinnamic acid. Among the tested mixtures, the highest AA was observed in mixture No. 8 and mixture No. 3, regardless of the assessment method. Consequently, to achieve the highest AA in a mixture, a higher content of quercetin and a minimal amount of trans-cinnamic acid should be included, while the optimal amount of rutin could not be determined. It is assumed that the amount of rutin should be at least equal to the amount of trans-cinnamic acid and ideally equal to the amount of quercetin. Thus, when developing dietary supplements with high AA, it is more advisable to include only quercetin rather than mixtures that also contain rutin and trans-cinnamic acid.

plant biology↗

bioRxiv2024

Assessment of the hypoglycemic activity of trans-cinnamic acid isolated from the extract of hairy rots Scutellaria baicalensis in in vivo experiments

AnnotationHypoglycemia is the process of reducing blood glucose concentration to below 2.5-2.8 mmol/L in men and less than 1.9-2.2 mmol/L in women. Prolonged hyperglycemia can lead to a range of serious chronic complications such as diabetic nephropathy, diabetic retinopathy, cardiovascular diseases in diabetes, and so forth, threatening human life, health, and safety. Scutellaria baicalensis is one of the most commonly used remedies in traditional Chinese medicine. This plant contains a number of biologically active compounds that contribute to improving kidney function, insulin resistance, and retinopathy in patients with type 2 diabetes, but few researchers study the toxic effects of medicinal plant components. Therefore, the aim of this study was to evaluate the hypoglycemic activity of trans-cinnamic acid isolated from the extract of hairy rots of Scutellaria baicalensis in in vivo experiments. This study involved intraperitoneal administration of alloxan at a dose of 150.0 mg/kg and oral administration of trans-cinnamic acid at doses of 50.0 and 100.0 mg/kg. During the experiment, it was found that trans-cinnamic acid at the presented doses does not affect the body weight dynamics of experimental animals with diabetes. Thus, this study demonstrates that trans-cinnamic acid at the presented dosage can be safely used as an ingredient in the creation of dietary supplements for the prevention of diabetes, thereby contributing to healthy aging.

developmental biology↗

bioRxiv2024

Study of the hepatotoxicity of trans-coric acid isolated from Scutellaria baicalensis in in vivo experiments

Biologically active supplements (BAS) based on plant material, possessing a wide range of beneficial properties (antioxidant, anti-inflammatory, antimicrobial, etc.), are becoming increasingly popular worldwide. Such supplements are readily available and can be used without consulting a healthcare professional. Consequently, the extent of their use is underestimated by doctors. Plant hepatotoxicity is not uncommon, but the frequency and manifestation are not sufficiently characterized. This study is dedicated to investigating the hepatotoxicity of trans-coric acid isolated from the root culture of Scutellaria baicalensis in in vivo experiments. This work aims to evaluate the hepatotoxicity of trans-coric acid on male rats at doses of 50.0 and 100.0 mg/kg. During the experiment, it was found that trans-coric acid, isolated from the root culture extract of Scutellaria baicalensis, does not induce hepatotoxicity in the presented doses in model animals with 14-day intragastric administration. Thus, this study demonstrates that trans-coric acid can be safely used as a component of BAS for the prevention of chronic diseases, thereby promoting healthy aging.

plant biology↗

bioRxiv2023

Effect of Mangiferin Isolated from Hedysarum neglectum L. Root Culture on Fat Accumulation in Experiments In Vivo

Plant polyphenols possess diverse medicinal and therapeutic properties. For instance, they can prevent such age-related diseases as stroke, heart attack, type II diabetes, and their various pathological complications. Mangiferin is a polyphenolic compound with a C-glycosylxanthone structure. This biologically active substance is known for its antioxidant, anti-inflammatory, antimicrobial, antidiabetic, and immunomodulatory properties. This research features mangiferin isolated from Hedysarum neglectum L. root culture and its effect on fat accumulation in Caenorhabditis elegans N2 Bristol. A set of experiments in vivo proved that mangiferin was able to inhibit fat accumulation in the model organism. The optimal concentration was determined as 50 m: it provided 44.5% fluorescence.

plant biology↗

bioRxiv2023

Effect of quercetin and kaempferol isolated from the Ginkgo biloba L. suspension culture extract on lipofuscin accumulation

Lipofuscin, known as an age pigment, is an autofluorescent lipopigment formed by lipids, metals, and misfolded proteins that accumulates in nerve cells, heart muscle cells, and skin. Individual bioactive compounds obtained from medicinal plant extracts reduce the level of lipofuscin in C. elegans nematodes in in vivo experiments. We aimed to study the effect of quercetin and kaempferol isolated from the in vitro suspension culture extracts of Ginkgo biloba L. on the accumulation of lipofuscin. According to the results, 200 M concentrations of these bioactive compounds showed the maximum decrease in lipofuscin levels in C. elegans N2 Bristol during their 15-day cultivation, compared to the control. Thus, our study showed that quercetin and kaempferol are able to reduce this age pigment in in vivo experiments.

developmental biology↗