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Prajapat, M.

Publications and source records attributed to Prajapat, M..

2 recordsLinked to original sources

AGO2 slicing of a domesticated retrotransposon is necessary for normal vasculature development

Argonaute-mediated RNA slicing is an ancient genome-defense mechanism linked to transposon repression. Although RNAi is no longer a major repeat-control pathway in mammals, AGO2 has retained catalytic activity, and this activity is essential for postnatal viability. The physiological targets that explain this requirement have remained unknown. Here we show that mammalian AGO2 slicing is required to restrain Rtl1, an imprinted domesticated retrotransposon whose cleavage is directed by miRNAs from the miR-433[~]127 cluster. Loss of AGO2 catalysis derepresses Rtl1 and causes endothelial defects and lethal neonatal muscle failure. Mechanistically, RTL1 is an oligomerization-prone retrotransposon-derived protein whose excess expression in muscle induces a non-aggregate proteostasis-stress state characterized by increased proteasome-dependent activity, reduced nascent protein synthesis, and depletion of mitochondrial, calcium-handling, and contractile gene programs. Thus, an essential function of mammalian AGO2 catalysis is to restrain a domesticated transposon-derived protein whose uncontrolled expression compromises endothelial and skeletal muscle function. These findings reveal how an ancestral transposon-Argonaute regulatory interaction has been repurposed to support mammalian development and survival.

developmental biology↗

The small non-coding RNA Vaultrc5 is dispensable to mouse development

Vault RNAs (vRNAs) are evolutionarily conserved small non-coding RNAs transcribed by RNA polymerase lll. Initially described as components of the vault particle, they have since also been described as noncanonical miRNA precursors and as riboregulators of autophagy. As central molecules in these processes, vRNAs have been attributed numerous biological roles including regulation of cell proliferation and survival, response to viral infections, drug resistance, and animal development. Yet, their impact to mammalian physiology remains largely unexplored. To study vault RNAs in vivo, we generated a mouse line with a conditional Vaultrc5 loss of function allele. Because Vaultrc5 is the sole murine vRNA, this allele enables the characterization of the physiological requirements of this conserved class of small regulatory RNAs in mammals. Using this strain, we show that mice constitutively null for Vaultrc5 are viable and histologically normal but have a slight reduction in platelet counts pointing to a potential role for vRNAs in hematopoiesis. This work paves the way for further in vivo characterizations of this abundant but mysterious RNA molecule. Specifically, it enables the study of the biological consequences of constitutive or lineage-specific Vaultrc5 deletion and of the physiological requirements for an intact Vaultrc5 during normal hematopoiesis or in response to cellular stresses such as oncogene expression, viral infection, or drug treatment.

genetics↗