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Popp, S.

Publications and source records attributed to Popp, S..

3 recordsLinked to original sources

A comparison of turn identification methods on high-frequency movement trajectories reveals potential comparability issues between studies of movement ecology

High-frequency animal tracks must often be subsampled to allow a simple analysis of the movement on the most meaningful scale for the respective study. One way of achieving this is to identify biologically significant turns, compared to heading changings caused by noise. Many turn identification methods have been developed, but the accuracy and consistency of such methods have rarely been validated against ground truth trajectories with known true turns and noise. We analyze simulated tracks with known parameters as well as two empirical tracks and identify turns with 10 different frequently used resampling methods. We assess the specificity and sensitivity of identifying the location of turns and compare the known mean step length and turn angle of the paths with the resampled trajectories. We found great accuracy differences between, and sometimes within, methods, even on simulated tracks of the same characteristics. Results of some methods were also highly sensitive to the user-set threshold the method requires (e.g. max angle). Overall, the best-performing methods in this study were DP and MRPA, methods used in human mobility research, and TPA, which is mostly used in primate research. We thus advise caution when comparing results of studies using different resampling methods and recommend justifying the use of the resampling method in addition to quantifying the sensitivity of results to the threshold value. This study is also an appeal to authors of novel turn identification methods to consider thorough comparisons in different scenarios with a wide range of previous methods, including those developed outside the movement ecology discipline.

ecology↗

Inexperienced bumble bees are poor at securely landing regardless of flower orientation or presence of labellum

The mutualism between bees and flowers creates strong selection on both the structure of the flower and behavior of the bee to maximize pollination and foraging success, respectively. Previous research has primarily assessed the costs of foraging by quantifying the time and accuracy of search, and handling time of the flower. However, there is little attention given to the actual success of landing, and it is often not explicitly stated whether failed landing attempts are taken into consideration. We show here that landing attempts often are unsuccessful, especially in inexperienced bees. Orientation of artificial flowers in our experiment neither influenced the preference nor landing success of a naive bumble bee forager. The presence of a labellum, often considered to serve as a landing platform, also did not influence landing success, indicating that it may mostly play a role in flower recognition or act as a nectar guide. Failed landing attempts may thus play an under-recognized role in the foraging efficiency and behavior of bees, and learning may be key in individual bee landing efficiency, not just flower recognition. Further research should aim to quantify the costs of landing failures and consider the role of experience in individual bee landing success.

animal behavior and cognition↗

ADAM17 Is The Main Sheddase For The Generation Of Human Triggering Receptor Expressed In Myeloid Cells (hTREM2) Ectodomain And Cleaves TREM2 After Histidine 157

Triggering receptor expressed in myeloid cells (TREM2) is a member of the immunoglobulin superfamily and is expressed in macrophages, dendritic cells, microglia, and osteoclasts. TREM2 plays a role in phagocytosis, regulates release of cytokine, contributes to microglia maintenance, and its ectodomain is shed from the cell surface. Using both pharmacological and genetic approaches we report here that the main protease contributing to the release of TREM2 ectodomain is ADAM17, (a disintegrin and metalloproteinase domain containing protein, also called TACE, TNF converting enzyme) while ADAM10 plays a minor role. Using mutational analysis, we demonstrate that the main cleavage site of the sheddases is located within the stalk region of TREM2 proximal to the plasma membrane. Complementary biochemical experiments reveal that cleavage occurs between histidine 157 and serine 158. Shedding is not altered for the R47H-mutated TREM2 protein that confers an increased risk for the development of Alzheimers disease. O-glycosylation is detected within the stalk region, but distant to the cleavage site. These findings reveal a link between shedding of TREM2 and its regulation during inflammatory conditions or chronic neurodegenerative disease like AD in which activity or expression of sheddases might be altered.

cell biology↗