Biology subjects

Pohlmann, T.

Publications and source records attributed to Pohlmann, T..

2 recordsLinked to original sources

bioRxiv2018

Core components of endosomal mRNA transport are evolutionarily conserved in fungi

Active movement of mRNAs by sophisticated transport machineries determines precise spatiotemporal expression of encoded proteins. A prominent example discovered in fungi is microtubule-dependent transport via endosomes. This mode of transport was thought to be only operational in the basidiomycete Ustilago maydis. Here, we report that distinct core components are evolutionarily conserved in fungal species of distantly related phyla like Mucoromycota. Interestingly, orthologues of the key RNA-binding protein Rrm4 from the higher basidiomycete Coprinopsis cinerea and the mucoromycete Rhizophagus irregularis shuttle on endosomes in hyphae of U. maydis. Thus, endosomal mRNA transport appears to be more wide-spread than initially anticipated.\n\nHighlights- Core transport components Upa1 and Rrm4 are conserved in different fungal phyla\n- Components of the Rrm4 machinery were most likely secondarily lost in ascomycetes\n- Upa1 from Microbotryum lychnidis-dioicae is functional in U. maydis\n- Rrm4 orthologues from Basidio- and Mucoromycota shuttle in hyphae of U. maydis

microbiology↗

bioRxiv2018

The multi PAM2 protein Upa2 functions as novel core component of endosomal mRNA transport

mRNA transport determines spatiotemporal protein expression. Transport units are higher-order ribonucleoprotein complexes containing cargo mRNAs, RNA-binding proteins and accessory proteins. Endosomal mRNA transport in fungal hyphae belongs to the best-studied translocation mechanisms. Although several factors are known, additional core components are missing. Here, we describe the 232 kDa protein Upa2 containing multiple PAM2 motifs (poly[A]-binding protein [Pab1] associated motif 2) as a novel core component. Loss of Upa2 disturbs transport of cargo mRNAs and associated Pab1. Upa2 is present on almost all transport endosomes in an mRNA dependent-manner. Surprisingly, all four PAM2 motifs are dispensable for function during unipolar hyphal growth. Instead, Upa2 harbours a novel N-terminal effector domain as important functional determinant as well as a C-terminal GWW motif for specific endosomal localisation. In essence, Upa2 meets all the criteria of a novel core component of endosomal mRNA transport and appears to carry out crucial scaffolding functions.

cell biology↗