Pierce, H. M. | bioRxiv Science

bioRxiv2022

COVID-19 Causes Ciliary Dysfunction as Demonstrated by Human Intranasal Micro-Optical Coherence Tomography Imaging

Severe acute respiratory syndrome coronavirus (SARS-CoV-2), causative agent of coronavirus disease 2019 (COVID-19), binds via ACE2 receptors, highly expressed in ciliated cells of the nasal epithelium. Micro-optical coherence tomography (OCT) is a minimally invasive intranasal imaging technique that can determine cellular and functional dynamics of respiratory epithelia at 1-m resolution, enabling real time visualization and quantification of epithelial anatomy, ciliary motion, and mucus transport. We hypothesized that respiratory epithelial cell dysfunction in COVID-19 will manifest as reduced ciliated cell function and mucociliary abnormalities, features readily visualized by OCT. Symptomatic outpatients with SARS-CoV-2 aged [≥] 18 years were recruited within 14 days of symptom onset. Data was interpreted for subjects with COVID-19 (n=13) in comparison to healthy controls (n=8). Significant reduction in functional cilia, diminished ciliary beat frequency, and abnormal ciliary activity were evident. Other abnormalities included denuded epithelium, presence of mucus rafts, and increased inflammatory cells. Our results indicate that subjects with mild but symptomatic COVID-19 exhibit functional abnormalities of the respiratory mucosa underscoring the importance of mucociliary health in viral illness and disease transmission. Ciliary imaging enables investigation of early pathogenic mechanisms of COVID-19 and may be useful for evaluating disease progression and therapeutic response. Graphical abstract O_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=94 SRC="FIGDIR/small/499336v1_ufig1.gif" ALT="Figure 1"> View larger version (35K): org.highwire.dtl.DTLVardef@1f4bb17org.highwire.dtl.DTLVardef@271438org.highwire.dtl.DTLVardef@1a08762org.highwire.dtl.DTLVardef@184847_HPS_FORMAT_FIGEXP M_FIG C_FIG

microbiology↗

bioRxiv2019

LITE-1 Mediates X-ray Avoidance Response in C. elegans

We report the finding that C. elegans display X-ray avoidance behavior at high but well tolerated doses, and that this behavior appears to require LITE-1, a gustatory receptor that has been implicated in UV avoidance behavior. We recorded acute behavioral responses of wildtype worms to increasing intensities of X-ray stimulation and found a positive stimulation-response relationship. Mutant strains of worms with dysfunctional photoreceptor proteins LITE-1 and GUR-3 were assayed, and the X-ray avoidance response was found to be nearly absent in LITE-1 mutants but not GUR-3 mutants, suggesting a prominent role for LITE-1 in the detection of X-rays. These findings may be important for developing optogenetics tools to stimulate cells in deep tissue using X-rays, for understanding the mechanism of LITE-1 signaling, and for understanding how organisms may respond to radiation.

neuroscience↗