Biology subjects

Peterson, J. K.

Publications and source records attributed to Peterson, J. K..

4 recordsLinked to original sources

bioRxiv2026

Live triatomine bug, vector of Trypanosoma cruzi (Chagas disease), found engorged in Lisbon hotel room: A first for Portugal and for Europe

Triatomine bugs are blood feeding insects that transmit the parasite Trypanosoma cruzi, causative agent of Chagas disease. The bugs are found primarily in the Americas with a few species in Asia and Africa. Here we report the first case of a live triatomine bug in Europe, found in a Lisbon hotel room. In August, 2025, the hotel room occupants discovered a triatomine bug perched on the headboard of their bed. Upon capture, bright red blood emerged from the bug; the occupants suspected that it had bitten them during the night. The bug was identified morphologically as triatomine species Hospesneotomae protracta, which was confirmed molecularly. Hospesneotomae protracta is native to the southwestern United States where it is a competent T. cruzi vector. Trypanosoma cruzi was not detected in this specimen. Although this case likely represents an accidental importation, it illustrates the ease with which disease vectors can be unknowingly transported globally. Ergo it is crucial to document and share these findings to prevent introductions of non-native arthropods of medical importance.

ecology↗

bioRxiv2024

Genome report: First whole genome sequence of Triatoma sanguisuga (Le Conte, 1855), vector of Chagas disease

Triatoma sanguisuga is the most widespread triatomine bug species in the United States (US). The species vectors the human parasite Trypanosoma cruzi, which causes Chagas disease. Vector-borne Chagas disease is rarely diagnosed in the US, but T. sanguisuga has been implicated in a handful of cases. Despite its public health importance, little is known about the genomics or population genetics of T. sanguisuga. Here, we used long-read sequencing to assemble the first whole genome sequence for T. sanguisuga using DNA extracted from one adult specimen from Delaware. The final size of the genome was 1.162 Gbp with 77.7x coverage. The assembly consisted of 183 contigs with an N50 size of 94.97 Kb. The Benchmarking Universal Single-Copy Ortholog (BUSCO) complete score was 99.1%, suggesting a very complete assembly. Genome-wide GC level was 33.56%, and DNA methylation was 18.84%. The genome consists of 61.4% repetitive DNA and 17,799 predicted coding genes. The assembled T. sanguisuga genome was slightly larger than that of Triatominae species Triatoma infestans and Rhodnius prolixus (949 Mbp with 90.4% BUSCO score and 706 Mbp with 96.5% BUSCO score, respectively). The T. sanguisuga genome is the first North American triatomine species genome to be sequenced, and it is the most complete genome yet for any Triatominae species. The T. sanguisuga genome will allow for deeper investigations into epidemiologically relevant aspects of this important vector species, including blood feeding, host seeking, and parasite competence, thus providing potential vector-borne disease management targets and strengthening public health preparedness.

genomics↗

bioRxiv2024

Hair follicles modulate skin barrier function

Our skin provides a protective barrier that shields us from our environment. Barrier function is typically associated with interfollicular epidermis; however, whether hair follicles influence this process remains unclear. Here, we utilize a potent genetic tool to probe barrier function by conditionally ablating a quintessential epidermal barrier gene, Abca12, which is mutated in the most severe skin barrier disease, harlequin ichthyosis. With this tool, we deduced 4 ways by which hair follicles modulate skin barrier function. First, the upper hair follicle (uHF) forms a functioning barrier. Second, barrier disruption in the uHF elicits non-cell autonomous responses in the epidermis. Third, deleting Abca12 in the uHF impairs desquamation and blocks sebum release. Finally, barrier perturbation causes uHF cells to move into the epidermis. Neutralizing Il17a, whose expression is enriched in the uHF, partially alleviated some disease phenotypes. Altogether, our findings implicate hair follicles as multi-faceted regulators of skin barrier function.

developmental biology↗

bioRxiv2020

A real-time search strategy for finding urban disease vector infestations

Containing domestic vector infestation requires the ability to swiftly locate and treat infested homes. In urban settings where vectors are heterogeneously distributed throughout a dense housing matrix, the task of locating infestations can be challenging. Here, we present a novel stochastic compartmental model developed to help locate infested homes in urban areas. We designed the model using infestation data for the Chagas disease vector species Triatoma infestans in Arequipa, Peru. Our approach incorporates disease vector counts at each observed house, and the vectors complex spatial dispersal dynamics. We used a Bayesian method to augment the observed data, estimate the insect population growth and dispersal parameters, and determine posterior infestation probabilities of households. We investigated the properties of the model through simulation studies, followed by field testing in Arequipa. Simulation studies showed the model to be accurate in its estimates of two parameters of interest: the growth rate of a domestic triatomine bug colony and the probability of a triatomine bug successfully invading a new home after dispersing from an infested home. When testing the model in the field, data collection using model estimates was hindered by low household participation rates, which severely limited the algorithm and in turn, the models predictive power. While future optimization efforts must improve the models capabilities when household participation is low, our approach is nonetheless an important step toward integrating data with predictive modeling to carry out evidence-based vector surveillance in cities.

bioinformatics↗