Biology subjects

Pessoa, G. C. D.

Publications and source records attributed to Pessoa, G. C. D..

4 recordsLinked to original sources

bioRxiv2025

Diversity of trypanosomatids in small mammals from a forest fragment at the wildlife-urban interface in southeastern Brazil: implications for public health surveillance

This study investigated the diversity of trypanosomatids infecting small mammals in a fragmented forest landscape in southeastern Brazil, aiming to clarify their role in transmission cycles and assess potential public health risks. Eighteen individuals from three species - Didelphis albiventris (n = 8), Marmosops incanus (n = 4), and Nectomys squamipes (n = 6) - were captured in the Mata da Tapera Municipal Natural Park (MT), located within the Espinhaco Range Biosphere Reserve, Minas Gerais State. Xenodiagnosis using Lutzomyia longipalpis and Rhodnius neglectus, followed by 18S rDNA nested PCR, enabled the detection of Leishmania infantum, L. braziliensis, and Trypanosoma cruzi DTU TcI in insects fed on naturally infected hosts. Tissue and organ samples were also analyzed by in vitro culture and molecular diagnostics. Leishmania infantum was identified in D. albiventris (n = 2), L. braziliensis in M. incanus (n = 1), T. cruzi TcI in D. albiventris (n = 2), and Trypanosoma lainsoni in both D. albiventris and N. squamipes (n = 1 each), the latter representing the first record of this parasite in that rodent species. This integrated approach provides a valuable model for zoonotic surveillance at the wildlife-urban interface, enhancing diagnostic sensitivity and species identification. These findings confirm the presence of zoonotic trypanosomatids in a highly anthropized landscape and highlight transmission risks to humans and domestic animals, particularly in an area with active ecotourism, underscoring the need for targeted surveillance within a One Health framework. Author summaryIn many parts of Brazil, forested areas are getting smaller and more fragmented due to urban growth. These changes increase the contact between wild animals, domestic animals, and humans, creating new opportunities for diseases to spread. In this study, we examined small wild mammals living in a forest fragment close to human settlements in southeastern Brazil to find out if they carried parasites that can also infect humans. We used a combination of techniques, including allowing laboratory-raised insects to feed on these animals, to detect the presence of different parasites. We found that these mammals were naturally infected with species of Leishmania and Trypanosoma, including those that cause leishmaniasis and Chagas disease in humans. We also detected Trypanosoma lainsoni, a lesser-known parasite, for the first time in one rodent species. Our findings suggest that small mammals living in areas where forests meet cities may play an important role in maintaining and spreading parasites that affect public health. This study shows the importance of monitoring wildlife in changing environments and supports the idea that human, animal, and environmental health are deeply connected.

molecular biology↗

bioRxiv2024

Deployment and transcriptional evaluation of nitisinone, an FDA-approved drug, to control bed bugs

Bed bugs are blood-feeders that rapidly proliferate into large indoor infestations. Their bites can cause allergies, secondary infections and psychological stress, among other problems. Although several tactics for their management have been used, bed bugs continue to spread worldwide wherever humans reside. This is mainly due to human-mediated transport and their high resistance to several classes of insecticides. New treatment options with novel modes of action are required for their control. In this study, we evaluated the use of nitisinone (NTBC), an FDA-approved drug, for bed bug control in an insecticide-susceptible (HH) and an insecticide-resistant (CIN) population. Although NTBC was lethal to both populations when administered orally or applied topically in very low doses, we observed a slight but significant resistance in the CIN population. Transcriptomic analysis in both populations indicated that NTBC treatment elicited a broad suppression of genes associated with RNA post-transcriptional modifications, translation, endomembrane system, protein post-translational modifications and protein folding. The CIN population exhibited higher ATP production and xenobiotic detoxification. Feeding studies on a mouse model highlight that NTBC could be used as a control method of bed bugs by host treatment. The results demonstrate that NTBC can be used as a new active ingredient for bed bug control by topical or oral treatment and shed light on the molecular mechanisms of suppressed tyrosine metabolism following NTBC treatment.

physiology↗

bioRxiv2019

The Drosophila fertility factor kl-3 is linked to the Y-chromosome of the vector of Chagas’ disease Triatoma infestans (Hemiptera: Reduviidae) and is essential for male fertility

In many insects, the Y chromosome plays a key role in sexual determination and male fertility. The Chagas disease vector Triatoma infestans has 22 autosomal chromosomes and a pair of XY sex chromosomes. However, the knowledge on the Y chromosome of this species, its genetic content or its biological function, is very poor. Due to repetitive DNA, Y chromosome sequences are poorly assembled in genome projects, hindering structural and functional studies on Y-linked genes. Our group has developed many of the bioinformatic tools to identify Y-linked sequences in assembled genomes. Here, we describe the identification of a {gamma}-dynein heavy chain linked to the Y-chromosome of T. infestans. This protein is orthologous to the Drosophila melanogaster Y-linked gene kl-3. In D. melanogaster, dyneins of the Y chromosome are known as male fertility factors and their deletion causes male infertility. We performed knockdown of the kl-3 expression to ascertain its function in T. infestans. Our results showed that injection of dsKL3 reduced, significantly, the fertility of T. infestans males (p<0.01). The mean number of eggs laid by the control group was 35.64 eggs/couple while the kl-3 knockdown group was of 11.82 eggs/couple (five couples did not lay any eggs). Differences in eclosion rate was even more significant, with a hatching mean rate of 16.85{+/-}10.03 and 1.69{+/-}3.58 (p<0.001) for the control and the silenced groups respectively. Our results suggest that kl-3 maintains its functional role as essential for male fertility in T. infestans. Hence, it seems that the Y-chromosome of T. infestans has a key role in male fertility. This is the first report of a kl-3 orthologue linked to the Y chromosome of an insect species outside the diptera clade. In addition to the first report of a Y-linked gene in T. infestans with a role for male fertility, this finding is of great relevance for the study of the evolution of Y chromosomes and further studies that could lead to novel approaches in insect control.

genomics↗

bioRxiv2019

Analysis of the testis transcriptome of the Chagas’ disease vector Rhodnius prolixus

Rhodnius prolixus is amongst the most important vectors of Trypanosoma cruzi in the Americas, putting thousands of people at risk of contracting Chagas Disease. This insect is also one of the most important models in insect physiology, especially regarding the blood-feeding process. However, studies on R. prolixus genetics lagged, and our understanding on the regulation of gene expression is incipient. Transcriptomes have the power to study the expression of thousands of genes in a single experiment. A comprehensive R. prolixus transcriptome was performed in 2014, sequencing RNA from different tissues (anterior gut, midgut, posterior gut, rectum, ovaries, fat body, maphigian tubules, and testicles). However, on that occasion, only the gut transcriptome was deeply analysed. Here we evaluated the results of the testicles transcriptome of R. prolixus with the objective to find and understand genes that could have an important role in male reproduction. We found, that from the 25,673 transcripts assembled in the whole transcriptome, 5,365 have a testicle specific expression pattern. As expected, amongst the most abundant families of transcripts, are those related to spermatogenesis and male fertility, such as myosins, actins, and dyneins. To our surprise, lipocalins, serine protease inhibitors (serpins), and lysozymes also were highly abundant in testicles. The role of these classes of genes are well known in other tissues, such as salivary glands and gut, but very little is known on their role in male reproduction (and we proposed here a few hypothesis that could be tested to address the role of these genes in male fertility). It would be interesting to study further the role of these genes on R. prolixus male fertility. Finally, as a reflection of the lack of knowledge on triatomine genetics, we found that almost half of the transcripts in R. prolixus testicles have no similarities to any other genes on reference databases. Our study shows that we still have a lot to know and to understand about reproduction in triatomine, especially in males. Besides the large number of genes without described function (possibly novel genes), there are those in which the function is known for other tissues, and we can only guess, at best, the role and importance of such genes for triatomine male fertility. Author SummaryThe understanding of the biology of insects vectors of parasitic diseases is key to the development of strategies of public health. For decades, the studies on the biology of male insects vectors of diseases was neglected, since in many cases female insects are those with relevant role in the spread of diseases. With the development of genomics, large scale studies to compare differential gene expression (transcriptomics) among different tissues, developmental stages, and sex became accessible. In this study, we looked at the physiology of the male reproductive organs of the vector of Chagas disease Rhodnius prolixus. This is a first glimpse, from a perspective of genes differentially expressed in male gonads, in such insects. We also performed an effort to link all identified genes with the insect genome published in 2015. We found ~14,000 genes expressed in the testicles, from which 5,635 genes are expressed exclusively in male reproductive organs. From the ~14,000 genes, we were able to attribute putative biological functions to 6,372 genes, which allowed us to draw a bigger picture on how these genes contribute to male fertility. This study now opens the door for further in-depth studies to find key genes for R. prolixus reproductive biology.

genomics↗