Peeters, K. | bioRxiv Science

bioRxiv2024

Bridging immunotypes and enterotypes using a systems immunology approach

Unveiling the systemic effects of disease and health requires an holistic approach that has mainly revolved around well established, directly determinable molecular relationships such as the protein synthesis cascade and epigenetic mechanisms. In this study, involving 394 individuals, we found direct linkage of branches spanning human biological functions often not studied in conjunction, using clinical data, gut microbial abundances, blood immune cell repertoires, blood transcriptomic and blood T cell receptor data. Contrary to current paradigms, we demonstrate that immunotypes and enterotypes are orthogonal, likely fulfilling distinct roles in maintaining homeostasis, only bridged via the blood transcriptome. We also identified two distinct inflammatory profiles: the first driven by interferon signalling and the other characterised by non-viral, NF-kB and IL-6 markers. Lastly, we present compelling data showing strong associations of the Ruminococcaceae and Christensenellaceae bacteria with a healthy immunotype and transcriptomic pattern, highlighting their potential role in immune health. Graphical Abstract O_FIG O_LINKSMALLFIG WIDTH=194 HEIGHT=200 SRC="FIGDIR/small/625344v1_ufig1.gif" ALT="Figure 1"> View larger version (52K): org.highwire.dtl.DTLVardef@c6b5eorg.highwire.dtl.DTLVardef@15d0c3forg.highwire.dtl.DTLVardef@1cf29eeorg.highwire.dtl.DTLVardef@1c9ff8d_HPS_FORMAT_FIGEXP M_FIG C_FIG

systems biology↗

bioRxiv2023

In-depth characterization of HINT1 pathogenic variants

BackgroundBiallelic loss-of-function variants in HINT1 cause neuromyotonia-associated axonal neuropathy (NMAN). Affected patients present from an early onset with a motor-greater-than-sensory polyneuropathy that is currently incurable. NMAN is a global cause of inherited peripheral neuropathy with higher prevalence in Europe and Asia. Nearly 30 distinct NMAN-associated variants have been reported to date, mostly in sporadic patients and small families. There is limited functional characterization of most of them, resulting in limited knowledge on their pathogenic mode of action and hindering the development of therapeutical strategies. MethodsWe systematically (re-)evaluated the pathogenicity of all reported HINT1 variants associated with NMAN using several in silico pathogenicity predictors used in the standard of care genetic testing. Fifteen missense and one truncating variants were further mapped onto the HINT1 crystal structure and grouped according to their spatial distribution. We combined several structural modeling tools to assess the impact of the variants on protein stability in both monomeric and dimeric forms. These variants underwent further functional validation by immunoblotting in both HeLa HINT1 knockout cell lines and in a S. cerevisiae strain deficient for the HINT1 orthologue. Finally, we tested the in vivo functionality of each variant by its ability to rescue yeast growth under stress conditions. ResultsOur combinatorial approach allowed the systematic characterization and (re-)evaluation of all known HINT1 variants associated with NMAN, enabling improved pathogenicity classification. Additionally, this standardized functional assessment allowed their categorization into four structure-based groups: 1) truncating variants; 2) variants of the catalytic pocket; 3) variants at the dimer interface; 4) variants in the distal {beta}-hairpin and nearby loop. Functional tests in yeast and mammalian disease models demonstrated their detrimental- yet differential -effect on HINT1 function, enabling structure-function correlations. ConclusionsOur standardized experimental pipeline allows for the characterization of newly discovered HINT1 variants in the context of NMAN, facilitating their pathogenicity interpretation. This work highlights the value of combining structural and functional approaches to understand better the underlying disease mechanisms. Our findings provide the rational basis for patient stratification and the development of personalized treatment strategies.

neuroscience↗