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Pearson, G. A.

Publications and source records attributed to Pearson, G. A..

3 recordsLinked to original sources

SAMMBA is a high-throughput pipeline for isolating and phenotyping macroalgal strains

Anthropogenic climate change is causing the decline of seaweed forests in many parts of the world. Despite successful preservation efforts, their immense biodiversity is still severely underrepresented in germplasm biobanks throughout the world. These culture libraries can preserve genetic diversity and provide inoculum for marine forest restoration and mariculture ventures, and potentially accelerate the selection and breeding of climate-resilient and high-yielding strains. However, the complex life cycles and body plans of seaweeds pose a huge challenge for the development of standardized phenotyping and isolating protocols for microscopic stages, especially with the efficiency necessary to deal with the current pace of global climatic changes. Here, we present SAMMBA (Seaweed Automatable Microplate Microscopy for Breeding Approaches), an end-to-end pipeline for the high-throughput isolation, phenotyping and storage of macroalgal cells in 384-well plates (384WP). By optimizing fluorescence microscopy imaging and analysis, along with a novel fragmentation method and dilution-to-extinction isolation, different unialgal seaweed tissues could be regrown after thousand-fold dilutions. In a single plate, we successfully isolated 68 singlet gametophyte fragments of Laminaria ochroleuca (39 males, 29 females; 17.7% efficiency) and 60 spores of Phyllariopsis purpurascens (31.25% efficiency). Furthermore, the taxonomic versatility of SAMMBA was demonstrated through the successful isolation of 60 unialgal cultures of red algae (Halymenia sp., Hydrolithon sp., Erythrotrichia sp.) and 10 strains of the green alga Ulva sp, without cross-contamination. The viability and unialgal nature of the isolated strains were verified by distributing a single L. ochroleuca strain across an entire 384-well plate and imaging each well over 30 days. We found that the average specific daily growth rates (daily SGR) per well were 0.130 {+/-} 0.006 and 0.117 {+/-} 0.01 day-1 for males and females, respectively, showing a significant difference between sexes (n = 768; p = 1.27e-53), while edge effects significantly reduced daily SGR in males but not in females. This approach dramatically increases experimental reproducibility and statistical power compared to conventional methods. Due to its modular design and cost-effectiveness, SAMMBA is readily adaptable to macroalgal repositories globally. It supports high-throughput, selective recovery of unialgal strains without reliance on robotic platforms, while remaining fully compatible with automation. This system significantly expands the experimental and operational capacity in macroalgal hatcheries, providing a scalable foundation for phenomics, domestication programs, and standardized, verifiable biobanking of unialgal strains. Ultimately, SAMMBA could provide critical support for breeding strategies required to ensure the resilience of marine forests and aquaculture in a rapidly changing ocean.

plant biology↗

Origin and evolutionary trajectories of brown algal sex chromosomes

Sex chromosomes fall into three classes: XX/XY, ZW/ZZ and U/V systems. The rise, evolution and demise of U/V systems has remained an evolutionary enigma. Here, we analyse genomes spanning the entire brown algal phylogeny to decipher their sex-determination evolutionary history. U/V sex chromosomes emerged between 450 and 224 million years ago, when a region containing the pivotal male-determinant MIN located in a discrete region in proto-U and proto-V chromosomes ceased recombining. Over time, nested inversions led to step-wise expansions of the sex locus, accompanying increasing morphological complexity and sexual differentiation of brown seaweeds. Unlike XX/XY and ZW/ZZ, brown algal U/V evolve mainly by gene gain, showing minimal degeneration. They are structurally dynamic and act as genomic cradles fostering the birth of new genes, potentially from ancestrally non coding sequences. Our analyses demonstrate that hermaphroditism arose from ancestral males that acquired U-specific genes by ectopic recombination, and that in the transition from a U/V to an XX/XY system, V-specific genes moved down the genetic hierarchy of sex determination. Both events lead to the demise of U and V and erosion of their specific genomic characteristics. Taken together, our findings offer a comprehensive model of U/V sex chromosome evolution.

evolutionary biology↗

Sex-dependent and -independent transcriptional changes during haploid phase gametogenesis in the sugar kelp Saccharina latissima

In haplodiplontic lineages, sexual reproduction occurs in haploid parents without meiosis. Although widespread in multicellular lineages such as brown algae (Phaeophyceae), haplodiplontic gametogenesis has been little studied at the molecular level. We addressed this by generating an annotated reference transcriptome for the gametophytic phase of the sugar kelp, Saccharina latissima. Transcriptional profiles of microscopic male and female gametophytes were analysed at four time points during the transition from vegetative growth to gametogenesis. Gametogenic signals resulting from a switch in culture irradiance from red to white light activated a core set of genes in a sex-independent manner, involving rapid activation of ribosome biogenesis, transcription and translation related pathways, with several acting at the post-transcriptional or post-translational level. Additional genes regulating nutrient acquisition and key carbohydrate-energy pathways were also identified. Candidate sex-biased genes under gametogenic conditions had potentially key roles in controlling female- and male-specific gametogenesis. Among these were several sex-biased or - specific E3 ubiquitin-protein ligases that may have important regulatory roles. Females specifically expressed several genes that coordinate gene expression and/or protein degradation, and the synthesis of inositol-containing compounds. Other female-biased genes supported parallels with oogenesis in divergent multicellular lineages, in particular reactive oxygen signalling via an NADPH-oxidase. Males specifically expressed the hypothesised brown algal sex-determining factor. Male-biased expression mainly involved upregulation of genes that control mitotic cell proliferation and spermatogenesis in other systems, as well as multiple flagella-related genes. Our data and results enhance genome-level understanding of gametogenesis in this ecologically and economically important multicellular lineage.

genomics↗