Biology subjects

Pavlou, C.

Publications and source records attributed to Pavlou, C..

3 recordsLinked to original sources

bioRxiv2025

Ultrasound Transparent Neural Interfaces for Multimodal Interaction

Neural interfaces that unify diagnostic and therapeutic functionalities hold particular promise for advancing both fundamental neuroscience and clinical neurotechnology. Functional ultrasound imaging (fUSI) has recently emerged as a powerful modality for high-resolution, non-invasive monitoring of brain function and structure. However, conventional metal-based microelectrodes typically impede ultrasound propagation, limiting compatibility with fUSI. Here, we present flexible, ultrasound-transparent neural interfaces that retain practical metal thicknesses while achieving high acoustic transparency. We introduce a theoretical and simulation-based framework to investigate the conditions under which commonly used polymers and metals in neural interfaces can become acoustically transparent. Based on these insights, we propose design guidelines that maximize ultrasound transmission through soft neural interfaces. We experimentally validate our approach through immersion experiments and by demonstrating the acoustic transparency of a suitably engineered interface using fUSI in phantom and in vivo experiments. Finally, we discuss the potential extension of this approach to therapeutic focused ultrasound (FUS). This work establishes a foundation for the development of multimodal neural interfaces with enhanced diagnostic and therapeutic capabilities, enabling both scientific discovery and translational impact.

bioengineering↗

bioRxiv2025

Transparent transfer-free multilayer graphene microelectrodes enable high quality recordings in brain slices

Resolving the underlying mechanisms of complex brain functions and associated disorders remains a major challenge in neuroscience, largely due to the difficulty in mapping large-scale neural network dynamics with high temporal and spatial resolution. Multimodal neural platforms that integrate optical and electrical modalities offer a promising approach that surpasses resolution limits. Over the last decade, transparent graphene microelectrodes have been proposed as highly suitable multimodal neural interfaces. However, their fabrication commonly relies on the manual transfer process of pre-grown graphene sheets which introduces reliability and scalability issues. In this study, multilayer graphene microelectrode arrays (MEAs) with electrode sizes as small as 10-50 {micro}m in diameter, are fabricated using a transfer-free process on a transparent substrate for in vitro multimodal platforms. Through acute experiments using cerebellar brain slices, their ability to detect spontaneous extracellular spiking activity from neural cells, with a high signal-to-noise ratio up to 30-40 dB, is demonstrated. The recorded signal quality is found to be more limited by the electrode-tissue coupling than the MEA technology itself. Overall, this study shows the potential of transfer-free multilayer graphene MEAs to interface with neural tissue, which paves the way to advance neuroscientific research through the next-generation of multimodal neural interfaces.

bioengineering↗

bioRxiv2024

The invasions of Aedes aegypti and Aedes albopictus in Cyprus: current situation, risk modelling and public health implications for the wider Eastern Mediterranean region.

The Asian tiger mosquito, Aedes albopictus and the yellow fever mosquito, Aedes aegypti have been spreading worldwide and are reshaping the distribution of arboviruses. Both Aedes species have recently been observed in densely populated cities of Cyprus, a touristic island that is a historic bridge between Europe and Asia. Given the high public health stakes for Cyprus and the wider East Mediterranean region, the objectives of this study are three-fold. First, we present a novel delimitation strategy using spatially dense networks of ovitraps deployed in 500x500m cells in Limassol and Larnaca following the detection of Aedes species. Second, we use a dynamical vector model to estimate the potential of both species to spread further over Cyprus. Finally, we employ a basic reproduction number (R0) model to assess the potential transmission risk of arboviruses for the wider East Mediterranean region. Our results underline our delimitation strategys usefulness in delineating Ae. albopictus populations in Limassol and indicate the need for increased surveillance efforts for Ae. aegypti in Larnaca. Our vector model reveals that cities such as Nicosia, Paphos and Ayia Napa are climatically suitable for the establishment of both Ae. aegypti and Ae. albopictus. Finally, the R0 model captures historical hotspots of dengue transmission over the East Mediterranean region, with large R0 values simulated over Cyprus, Greece, Turkey, southern Italy and southern Spain. We recommend stringent vector surveillance at entry points in Greece and a rapid elimination in Cyprus to prevent the return of Ae. aegypti to the European continent.

ecology↗