Biology subjects

Pardo, M. A.

Publications and source records attributed to Pardo, M. A..

4 recordsLinked to original sources

bioRxiv2023

A rise in double-strand breaks sensitizes tumours to oxidative metabolism inhibitors

ABSTRACTDouble strand brakes (DSB) accumulate in cellular DNA as a result of deficiencies in homologous recombination repair systems, such as mutations in BRCA genes, or upon antitumoral treatments. In the present study we show that the accumulation of DSB, regardless of its origins, leads to a shift towards oxidative metabolism. We have identified that DSB-induced reactive oxygen species (ROS) promote the activation of NRF2 which downregulates the glycolytic transcription factor HIF-1. HIF-1 inhibition is a key step in this metabolic shift, because leads to the reduction of PDHK1 levels and the consequential activation of pyruvate dehydrogenase, a mitochondrial gatekeeper of cellular metabolism, promoting this metabolic shift. Remarkably, after the induction of DSBs, the tumour is more sensitive to the inhibition of oxidative metabolism since both treatments synergize in vivo, resulting in reduced tumour growth. Therefore, we demonstrate a significant feedback between DSBs induction and cancer cell metabolism that ultimately limits the cells potential for metabolic plasticity, hence sensitizing it to the action of counteracting drugs.

cancer biology↗

bioRxiv2023

African elephants address one another with individually specific calls

Personal names are a universal feature of human language, yet few analogs exist in other species. While dolphins and parrots address conspecifics by imitating the calls of the addressee 1,2, human names are not imitations of the sounds typically made by the names owner 3. Labeling objects or individuals without relying on imitation of the sounds made by that object or individual is key to the expressive power of language. Thus, if non-imitative name analogs were found in other species, this could have important implications for our understanding of language evolution. Here, we show that wild African elephants address one another with individually specific calls without any evidence of imitating the receivers vocalizations. A random forest model correctly predicted receiver identity from call structure better than expected by chance, regardless of whether the calls were more or less similar to the receivers calls than typical for that caller. Moreover, elephants differentially responded to playbacks of calls originally addressed to them relative to calls addressed to a different individual, indicating that they can determine from a calls structure if it was addressed to them. Our findings offer the first evidence for a non-human species individually addressing conspecifics without imitating the receiver.

animal behavior and cognition↗

bioRxiv2020

The demographic decline of a sea lion population followed multi-decadal sea surface warming

BackgroundThe population growth of top predators depends largely on environmental conditions suitable for aggregating sufficient and high-quality prey. We reconstructed numerically the population size dynamics of a resident population of California sea lions in the Gulf of California during 1978 - 2019, and its relation with the gulfs multi-decadal sea surface temperature trend. This are the first long-term insights to the oceanic environment of the Gulf of California and to the population trend of one of its major predators. ResultsOur results indicate that a three-decade sustained warming explains statistically the populations trend, including a [~]65% decline between 1991 - 2019, accounting for 92% of the variance. Long-term warming conditions started in the late 80s, followed by the populations decline, from a peak of 43,834 animals (range: 34,080 - 58,274) in 1991 to only 15,291 (range: range: 11,861 - 20,316) in 2019. The models suggested a century-scale optimum sea surface habitat for the population occurring in mildly temperate waters, from 0.18 to 0.39{degrees}C above the 100- year mean of 22.24{degrees}C. ConclusionsThe negative relation of the population size with warming sea surface conditions was evident, and the predictability of the former from the sea surface temperature 100-year anomalies was high. The mechanistic links of this relation are still untested, but an apparent diversification of pelagic fish catches suggests a reduction of high quality prey. We propose that this sea lion population should be considered as vulnerable to any disturbance that could add to the negative effects of the current sea surface warming conditions in the Gulf of California.

ecology↗

bioRxiv2019

Spatial variation in black-headed night monkey (Aotus nigriceps) vocalizations

Quantitative acoustic analysis has been used to decipher individual differences, population structure, and taxonomic diversity in numerous primate species. We previously described three distinct call types in wild Aotus nigriceps, and now assess acoustic differences in two of these call types between social groups and spatially distinct populations. Acoustic parameters for both analyzed call types exhibited significant variability between groups. Similarly, geographically distant field sites were acoustically distinct from one another. Several groups also used a variation of a common call: a triplet Ch Ch instead of a duplicate. Other groups made use of ultrasonic frequencies which have not previously been reported in Aotus. Our results suggest that Aotus nigriceps exhibits substantial acoustic variability across sites that could potentially be useful for taxonomic classification, although additional geographically distant populations still need to be sampled. The possibility of individual signatures also exists and will require recording vocalizations from known individuals.

animal behavior and cognition↗