Biology subjects

Pankratova, M.

Publications and source records attributed to Pankratova, M..

2 recordsLinked to original sources

bioRxiv2024

Testing various combinations of cryoprotective agents for human skin cryopreservation by vitrification method

Modern translational medicine, pharmacology and other fields require effective skin cryopreservation technology. Vitrification is promising technology for this aim. This rapid freezing technique minimizes hexagonal ice crystals formation, time of stress condition for cells in samples and dehydration both in the cytoplasm of cells and in the extracellular space. This study is aimed at finding the optimal combination of extracellular (sucrose, mannitol, polyethylene glycol and FBS) and intracellular (DMSO, glycerol, ethylene glycol) cryoprotective agents to achieve the highest viability and tissue structure preservation of skin samples after vitrification.

cell biology↗

bioRxiv2023

Testing of various protocols for human skin cryopreservation by vitrification method

Optimization of skin cryopreservation technology is an important task for translational medicine, pharmacology, and a list of other fields. One of the promising approaches is vitrification. This rapid freezing technique allows one to ensure minimal damage to the sample by equating the formation of ice crystals and dehydration both in the cytoplasm of cells and in the extracellular space. This study is aimed at finding the optimal combination of cryoprotective agents (DMSO, glycerol, sucrose and polyethylene glycol) to achieve the highest viability of skin samples after vitrification.

cell biology↗