Palfreyman, R. | bioRxiv Science

bioRxiv2025

Glycaemic variability underlies myocyte dysfunction and myocardial injury risk in diabetes

Heart disease is the leading cause of morbidity and mortality in individuals with diabetes, due largely to risks associated with ischaemic injuries such as myocardial infarction (MI). We use human population genetic data to demonstrate that current biomarkers of hyperglycaemia do not account for risk of post-MI mortality in diabetes patients. This study therefore systematically evaluates glycaemic stress underpinning cardiovascular risk in diabetes. Using in vivo and in vitro models, we demonstrate that glycaemic variability rather than hyperglycaemia alone is a dominant risk factor for heart muscle dysfunction and myocardial injury sensitivity in diabetes. These findings provide new preclinical models for mechanistic and drug discovery studies and inform strategies for managing cardiovascular outcomes in patients with diabetes.

cell biology↗

bioRxiv2025

Abundant and active acetogens enhance the carbon dioxide sink of Blue Carbon ecosystems

Blue Carbon ecosystems, which include all tidal wetlands, mitigate climate change by capturing and storing carbon dioxide (CO2) from the atmosphere. Most carbon fixation in these systems is thought to be driven by plant and microbial photosynthesis, whereas chemosynthetic processes are assumed to play a minor role. However, these ecosystems often contain anoxic environments ideal for chemosynthetic microbes such as acetogens. Here, we show that acetogens are abundant and active mediators of carbon sequestration in tidal wetland soils by pairing gene-and genome-resolved metagenomic analysis with isolation and analysis of gas-fermenting acetogens in bioreactors. Metagenomic profiling revealed that diverse microbes can mediate carbon fixation, primarily through the Calvin-Benson-Bassham cycle and Wood-Ljungdahl pathways. These include various bacteria and archaea capable of reductive acetogenesis. On this basis, we grew bacterial enrichment cultures from tidal wetland soils using the gases hydrogen and CO2 as the sole energy and carbon sources. Bioreactor analysis revealed that these enrichments are dominated by clostridial acetogens that grow rapidly by converting CO2 into acetate and other products. Collectively, these results reveal Blue Carbon ecosystems harbour communities that can exclusively subsist by using CO2 as their sole electron acceptor and for carbon fixation, thereby providing evidence of a novel carbon sink pathway within these ecosystems beyond the known mechanisms of photosynthetic carbon fixation and soil sequestration. Additionally, the discovery and isolation of these chemosynthetic communities provide opportunities for developing further mechanisms of CO2 removal through industrial gas fermentation.

microbiology↗