Osman, E. A. | bioRxiv Science

bioRxiv2026

Tunable gene expression in zebrafish using RiboSCALE

Chemogenetic tools enable conditional control of gene expression during embryonic development and regeneration. However, many conditional tools induce constitutive or one-way activity precluding temporal resolution of gene function or require the use of multiple transgenic lines. We developed an RNA-based chemogenetic approach to induce gene expression in zebrafish embryos and larvae. We demonstrate that a gene of interest can be turned on in a time-dependent and concentration-dependent manner. Using this approach, we have characterized two different aptamers for future investigation.

developmental biology↗

bioRxiv2018

Comprehensive in silico Analysis of IKBKAP gene that could potentially cause Familial dysautonomia

BackgroundFamilial dysautonomia (FD) is a rare neurodevelopmental genetic disorder within the larger classification of hereditary sensory and autonomic neuropathies. We aimed to identify the pathogenic SNPs in IKBKAP gene by computational analysis softwares, and to determine the structure, function and regulation of their respective proteins.\n\nMaterials and MethodsWe carried out in silico analysis of structural effect of each SNP using different bioinformatics tools to predict SNPs influence on protein structure and function.\n\nResult41 novel mutations out of 973 nsSNPs that are found be deleterious effect on the IKBKAP structure and function.\n\nConclusionThis is the first in silico analysis in IKBKAP gene to prioritize SNPs for further genetic studies.

bioinformatics↗