Olsen, A. J. | bioRxiv Science

bioRxiv2022

Analysis of the complete genome sequence for Halococcus dombrowskii ATCC BAA-364T

We describe sequencing and assembly of complete Halococcus dombrowskii H4T (=ATCC BAA-364T) genome using short- and long-read sequencing technologies. The first closed genome within its genus is composed of a 2,767,537 bp chromosome and five additional plasmids totalling 3,965,466 bp, with GC content of 62.18%. The genome contains 4,029 genes, 3,963 coding sequences and two CRISPR arrays. Unusually, this Euryarchaeote carries multiple rRNA operons with divergent ITS identities across both its chromosome and plasmids.

genomics↗

bioRxiv2020

Open source software tool for the automated detection and characterization of epithelial cells from trace biological samples

This paper presents a strategy for an unsupervised workflow for identifying epithelial cells in microscopic images and characterizing their morphological and/or optical properties. The proposed method can be used on cells that have been stained with fluorescent dyes and imaged using conventional optical microscopes. The workflow was tested on cell populations that were imaged directly on touch/contact surfaces and stained with nucleic acid dyes to visualize genetic content. Our results show that this approach could be a useful strategy for characterizing differences in staining efficiency and/or morphological properties of individual cells or aggregate populations within a biological sample. Further, they can potentially reduce the laborious nature of microscopic analysis and increase throughput and reproducibility of similar studies.

cell biology↗