Biology subjects

Oliveira, D. B. L.

Publications and source records attributed to Oliveira, D. B. L..

3 recordsLinked to original sources

bioRxiv2021

SARS-CoV-2 activates ER stress and Unfolded protein response

Coronavirus disease-2019 (COVID-19) pandemic caused by the SARS-CoV-2 coronavirus infection is a major global public health concern affecting millions of people worldwide. The scientific community has joint efforts to provide effective and rapid solutions to this disease. Knowing the molecular, transmission and clinical features of this disease is of paramount importance to develop effective therapeutic and diagnostic tools. Here, we provide evidence that SARS-CoV-2 hijacks the glycosylation biosynthetic, ER-stress and UPR machineries for viral replication using a time-resolved (0-48 hours post infection, hpi) total, membrane as well as glycoproteome mapping and orthogonal validation. We found that SARS-CoV-2 induces ER stress and UPR is observed in Vero and Calu-3 cell lines with activation of the PERK-eIF2-ATF4-CHOP signaling pathway. ER-associated protein upregulation was detected in lung biopsies of COVID-19 patients and associated with survival. At later time points, cell death mechanisms are triggered. The data show that ER stress and UPR pathways are required for SARS-CoV-2 infection, therefore representing a potential target to develop/implement anti-CoVID-19 drugs.

biochemistry↗

bioRxiv2020

Hypertonic saline solution inhibits SARS-CoV-2 in vitro assay

We are facing an unprecedented global health crisis caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). At this date more than 680 thousand people have died due to coronavirus disease 2019 (COVID-19). Unfortunately, until now no effective treatment to combat the virus and vaccine are available. We performed experiments to test if hypertonic saline solution is able to inhibit virus replication in vitro. Our data shows that 260 mM NaCl (1.5%) inhibits 100% SARS-CoV-2 replication in Vero cells. Furthermore, our results suggest that the virus replication inhibition is due to an intracellular mechanism and not due to the dissociation between spike SARS-CoV-2 protein and its human receptor angiotensin-converting enzyme 2 interaction. NaCl depolarizes the plasma membrane supposedly associated with the inhibition of the SARS-CoV-2 life cycle. This observation could lead to simple, safe and low cost interventions at various stages of COVID-19 treatment, improving the prognosis of infected patients, thereby mitigating the social and economic costs of the pandemic.

microbiology↗

bioRxiv2019

Potential Outbreak in Brazil by Equine Herpesvirus Type 2 (EHV-2): Detection, Clinical and Genetic Analysis of Equine Gammaherpesvirus 2.

Equine herpesvirus (EHV) commonly affects horses causing neurologic and respiratory symptoms beside spontaneous abortions, meaning huge economic losses for equine industry worldwide. In foals the virus can facilitate secondary infections by Rhodococcus equi, important in morbidity and mortality in equines. A total of five genotypes of EHV were previously described in Brazil including EHV-1, EHV-2, EHV-3, EHV-4 and EHV-5. EHV-2 genotype had only been previously described in Brazil in asymptomatic animals. We report the dead of 11 foals in Middle-west region of Brazil showing respiratory and neurological symptoms, as well as several abortions in mares from the same farm. Laboratory exams revealed neutrophilia leukocytosis. Necroscopic and histopathologic findings were suppurative bronchopneumonia and ulcerative enteritis. Molecular assays point to the absence of the bacteria Rhodococcus equi and other EHV. The presence of EHV-2 DNA was confirmed by sequencing in serum sample from one foal. This is the first confirmed outbreak of EHV-2 causing disease in Brazilian horses with confirmed presence of the virus. EHV-2 has been neglected and could be highlight the important role of EHV-2 in equine respiratory disease and spontaneous abortions in equid in Brazil.\n\nAuthor SummaryEquid herpesvirus type 2 (EHV-2), a gammaherpesvirus, has been neglected and can cause respiratory infection, abortion and in extreme cases neurologic symptoms and dead.\n\nOutbreaks of EHV-2 never was described in Brazil and can be critical to losses in equid industry. Our report provides evidence for a direct link between the EHV-2 strain and the outbreak in foals. We proved the clinical, histopathological and phylogenetic analysis of segment of EHV-2 polymerase.\n\nIn summary, our study confirm that Gammaherpesvirus 2 is circulating in Brazil and the EHV-2 is a cause of the first outbreak in foals showing the important role of EHV-2 in equid respiratory disease.

microbiology↗