Biology subjects

Obrepalska-Steplowska, A.

Publications and source records attributed to Obrepalska-Steplowska, A..

3 recordsLinked to original sources

bioRxiv2024

Symptom-modulating satRNAs of cucumoviruses affect the orientation and feeding behaviour of Myzus persicae

Plant viruses evolved mechanisms to manipulate host plants to replicate and be efficiently transmitted by insect vectors. In the context of non-persistently transmitted viruses, an important strategy is to change the plants metabolism to attract the vectors, and subsequently repel viruliferous insects from the infected plants to facilitate the virus transmission to neighbouring plants. The presence of satellite RNAs (satRNAs), which accompany certain plant RNA virus species and strains, leads to changes in the host plants, such as alterations in the virus-induced infection symptoms, either exacerbating or attenuating them. This study aimed to analyse the influence of satRNAs on the orientation and feeding behaviour of the cucumovirus insect vector - Myzus persicae, which might consequently contribute to the virus transmission efficiency. The hypothesis behind this study was that satRNAs of cucumoviruses alter these insect activities toward virus-infected plants, and strongly symptom-deteriorating satRNAs might negatively affect the attractiveness of the infected plants for aphids. Using two cucumoviruses, peanut stunt virus (PSV) and cucumber mosaic virus (CMV), and their satRNAs, which induce divergent infection symptoms, olfactometry and electrical penetration graph (EPG) monitoring analyses were performed. The results showed that satRNAs which presence leads to disease symptom exacerbation might alter the orientation behaviour of aphids by reducing the attractiveness of the plants and discouraging aphids from feeding. This phenomenon may contribute to the better persistence in the environment of satRNAs alleviating disease symptoms compared to the worsening ones, benefiting the virus by not destroying the plant and prolonging the virus exposure to insect vectors.

plant biology↗

bioRxiv2023

Profiling of Zea mays L. proteome at early stages of compatible interactions with Meloidogyne arenaria indicates changes in signaling, oxidative stress responses, and S-adenosylmethionine biosynthesis

Root-knot nematodes (RKNs) are distributed worldwide group of plant parasitic nematodes, with a very wide host range, including mono- and dicotyledonous hosts. Meloidogyne arenaria is, next to M. hapla, M. incognita, and M. javanica, one of the most economically important species from this genus. RKNs during parasitism hijack host metabolism to establish giant cells and to break down plant defense mechanisms. To date, studies on the interaction of RKN with maize (Zea mays L.) have been underrepresented, and a description of the early response to attack by these nematodes, vital to understanding the process, is scarce in the literature. We hypothesize that in the early stage of maize response to M. arenaria infection, significant changes in the accumulation level of proteins mainly related to plant defense response, plant cell wall modifications, and phytohormone biosynthesis can be observed. In this study, a mass spectrometry approach and a label-free quantification technique were used to assess the qualitative and quantitative composition of proteins changes in the proteome of maize roots after M. arenaria infection. We used a susceptible maize variety and carried out analyses of plant proteome at two time points: 24 hours after nematode inoculation and 3 days after inoculation. Statistical analyses of significant differences between protein intensities were performed for the datasets obtained from healthy and M. arenaria-infected plants, and differentially expressed proteins (DEPs), with both lower and higher abundance were determined. DEPs were mapped, classified to the gene ontology (GO) terms into functional categories, and assigned to appropriate Kyoto Encyclopedia of Genes and Genomes (KEGG) processes and pathways. As a result, a total of 3,743 proteins were identified with 124 DEPs at 24 hpi and 66 at 3 dpi, and significant changes in the accumulation of proteins associated with processes such as cell wall modifications, reaction to stress, as well as processes and pathways related to phenylpropanoid biosynthesis and metabolism, signal transduction and to S-adenosylmethionine biosynthesis.

plant biology↗

bioRxiv2022

Deciphering of BTH-induced response of tomato (Solanum lycopersicum L.) and its effect on plant virus infection through the multi-omics approach

One of the preventive methods used to limit the losses caused by viruses is the application of synthetic immunity inducers, such as benzo(1,2,3)-thiadiazole-7-carbothioic acid S-methyl ester (BTH). This study aimed to explain how the BTH treatment affects the defence and developmental processes in tomato plants (Solanum lycopersicum L.) as well as plant response to virus infection. The comparative multi-omics analyses of tomato plants treated with BTH were performed, including transcriptomics (RNA-seq), proteomics (Liquid Chromatography-Mass Spectrometry), and metabolomics (targeted hormonal analysis). To confirm the priming effect of BTH on tomato resistance, the plants were infected with tomato mosaic virus (ToMV) seven days post-BTH treatment. The combined functional analysis indicated the high impact of BTH on the plants developmental processes and activation of the immune response early after the treatment. In the presented experimental model, the increased level of WRKY TRANSCRIPTION FACTORS, ARGONAUTE 2A, thiamine and glutathione metabolism, cell wall reorganization, and detoxification processes, as well as accumulation of three phytohormones: abscisic acid, jasmonic-isoleucine (JA-Ile), and indole-3-carboxylic acid (I3CA), were observed upon BTH application. The immune response activated by BTH was related to increased expression of genes associated with the cellular detoxification process, systemic acquired resistance, and induced systemic resistance as well as post-transcriptional gene silencing. Increased levels of I3CA and JA-Ile might explain the BTHs effectiveness in the induction of the plant defence against a broad spectrum of pathogens. For the first time, the BTH impact on the thiamine metabolism was revealed in tomatoes.

plant biology↗