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Ober, R. J.

Publications and source records attributed to Ober, R. J..

2 recordsLinked to original sources

Resolution limit of image analysis algorithms

Resolution is one of the most important properties of an imaging system, yet it remains difficult to define and apply. Rayleighs and Abbes resolution criteria1 were developed for observations with the human eye and had a major influence on the development of optical instruments. However, no systematic approach is available for the evaluation of the often complex image processing algorithms that have become central to the analysis of the imaging data that today is acquired by highly sensitive cameras.\n\nMany modern imaging experiments are based on the detection of objects. Examples are localization-based superresolution experiments (PALM, STORM, etc.2-4), experiments to investigate the arrangement of molecular complexes on the cellular membrane such as clathrin-coated pits5,6, experiments tracking single particles7, ...

biophysics

Intensity-based axial localization approaches for multifocal plane microscopy

Multifocal plane microscopy (MUM) can be used to visualize biological samples in three dimensions over large axial depths and provides for the high axial localization accuracy that is needed in applications such as the three-dimensional tracking of single particles and superresolution microscopy. This report analyzes the performance of intensity-based axial localization approaches as applied to MUM data using Fisher information calculations. In addition, a new non-parametric intensity-based axial location estimation method, Multi-Intensity Lookup Algorithm (MILA), is introduced that, unlike typical intensity-based methods that make use of a single intensity value per data image, utilizes multiple intensity values per data image in determining the axial location of a point source. MILA is shown to be robust against potential bias induced by differences in the sub-pixel location of the imaged point source. The method's effectiveness on experimental data is also evaluated.

biophysics