Biology subjects

O Maoileidigh, D.

Publications and source records attributed to O Maoileidigh, D..

3 recordsLinked to original sources

bioRxiv2026

Trial-By-Trial Auditory Brainstem Response Detection

The neural response of the brainstem to brief sounds, known as the auditory brainstem response (ABR), is widely employed in the laboratory and the clinic to diagnose hearing loss. In contrast to behavioral methods that assess hearing using responses to sounds on a trial-by-trial basis, current ABR approaches are limited to analyzing the average ABR over hundreds of trials. Historically, trial-by-trial ABR analysis has not been possible owing to each trials small signal-to-noise ratio. Here we overcome this limitation and show how to classify individual ABR trials as detected or undetected. We use the distribution of single-trial ABRs to assess supra-threshold hearing and to define psychophysics-like thresholds, which we call auditory brainstem detection (ABD) thresholds. ABD thresholds decrease as more of the ABR epoch is taken into account, whereas traditional ABR thresholds do not change. Above the ABD thresholds and below 90 dB SPL, signal detection is significantly improved by utilizing more of the ABR epoch. Our method also allows us to rank the supra-threshold hearing ability of individual subjects. Despite having normal ABR thresholds, some subjects appear to have supra-threshold hearing deficits. The trial-by-trial method demonstrates that signal detection by the ensemble of auditory neurons in the brainstem is intrinsically stochastic not only at low stimulus levels, but also at levels up to 100 dB SPL. Significance StatementNeural responses to sound can be measured by electrodes placed on a subjects head and are commonly used in the laboratory and the clinic to assess hearing. Although the auditory system must distinguish each sound stimulus from intrinsic noise, current methods for ana-lyzing the response of the brainstem to sound only utilize the average response to hundreds of stimuli. Here we overcome this constraint by showing how to classify an individual sound stimulus as detected or undetected based on each auditory brainstem response. This ap-proach can assess hearing at all stimulus levels, indicates that subjects with normal hearing thresholds can exhibit supra-threshold hearing loss, and potentially extends the types of hearing deficits that can be diagnosed using auditory evoked potentials.

physiology↗

bioRxiv2024

Stereocilium height changes can account for the calcium dependence of the outer-hair-cell bundle's resting state

Outer-hair-cell bundles are sensory organelles required for normal hearing in mammals. These bundles convert sound-induced forces into receptor currents. This conversion depends on the resting receptor current of each bundle, which increases when extracellular calcium is decreased to the physiological level. How extracellular calcium regulates the bundles resting state is not well understood. We propose a mechanism explaining how extracellular calcium can regulate the outer-hair-cell bundles resting state. Each bundle comprises filamentous stereocilia linked by gating springs that are attached to ion channels. Sound-induced forces deflect stereocilia, increasing and decreasing gating-spring tensions, opening and closing the ion channels, resulting in an oscillating receptor current. We hypothesize that decreasing extracellular calcium, decreases the heights of the shorter stereocilia, increasing resting gating-spring tensions, which increases the resting receptor current and decreases the bundles resting deflection. To determine the plausibility of this mechanism, we build a mathematical model of an outer-hair-cell bundle and calibrate the model using seven independent experimental observations. The calibrated model shows that the mechanism is quantitatively plausible and predicts that a decrease of only 10 nm in the heights of the shorter stereocilia when extracellular calcium is lowered is sufficient to explain the observed increase in the resting receptor current. The model predicts the values of nine parameters and makes several additional predictions.

physiology↗

bioRxiv2018

Bilateral spontaneous otoacoustic emissions show coupling between active oscillators in the two ears

Spontaneous otoacoustic emissions (SOAEs) are weak sounds that emanate from the ears of tetrapods in the absence of acoustic stimulation. These emissions are an epiphenomenon of the inner ear's active process, which enhances the auditory systems sensitivity to weak sounds, but their mechanism of production remains a matter of debate. To understand the relationship between SOAEs that we recorded simultaneously from the two ears of the tokay gecko, we developed a mathematical model of the eardrums as noisy nonlinear oscillators coupled by the air within a lizards mouth. We found that binaural emissions could be strongly correlated: some emissions occurred at the same frequency in both ears and were highly synchronized. Suppression of the emissions in one ear often changed the amplitude or shifted the frequency of emissions in the other. Decreasing the frequency of emissions from one ear by lowering its temperature usually reduced the frequency of the contralateral emissions. By according with the model, the results indicate that some SOAEs are generated bilaterally through acoustic coupling across the oral cavity. The model predicts that sound localization through the acoustic coupling between ears is influenced by the active processes of both ears.

neuroscience↗