Noble, D. J. | bioRxiv Science

bioRxiv2025

Early Changes in Respiration and Motor Activity after Spinal Cord Injury Predict Pain-Related Outcomes in Mice

Spinal cord injuries (SCIs) may lead to the emergence of chronic pain many weeks after injury. Using the thoracic contusion model of SCI-induced neuropathic pain, we investigated whether early changes in mouse respiration or motor activity could predict and differentiate emergent dysfunction with focus on pain. We measured respiratory rate (RR) and movement (motor activity) in freely behaving mice before and at several time points following SCI. We then assessed behavioral signs of pain or thermal dysregulation by testing evoked hindpaw withdrawal responses to mechanical and thermal heating stimuli and temperature preferences at four weeks after injury. For 2-3 days after injury, mice exhibited sharp decreases in movement and RR variability, but these two parameters were uncorrelated within animals. Mice showed signs of mechanical and thermal hypersensitivity and preferences for warmer temperatures four weeks after injury. Interestingly, mice that moved the least 1 day after SCI preferentially underwent hindpaw mechano-sensitivity (r = 0.67, p =.036), whereas mice with large decreases in RR variability that recovered by 8 days post-injury preferred higher temperatures in the thermal preference test (r = 0.79, p =.007). Thus, early changes in movement and RR variability may differentially predict future hypersensitivity and thermal dysfunction. More broadly, early post-injury physio-behavioral events could inform novel interventions to mitigate subsequent emergent dysfunction after SCI.

physiology↗

bioRxiv2022

C-low threshold mechanoreceptor activation becomes sufficient to trigger affective pain in spinal cord-injured mice in association with increased respiratory rates

The mechanisms of neuropathic pain after spinal cord injury (SCI) are not fully understood. In addition to the plasticity that occurs within the injured spinal cord, peripheral processes, such as hyperactivity of primary nociceptors, are critical to the expression of pain after SCI. In adult rats, truncal stimulation within the tuning range of C-low threshold mechanoreceptors (C-LTMRs) contributes to pain hypersensitivity and elevates respiratory rates (RRs) after SCI. This suggests that C-LTMRs, which normally encode pleasant, affiliative touch, undergo plasticity to transmit pain sensation following injury. Because tyrosine hydroxylase (TH) expression is a specific marker of C-LTMRs, in the periphery, here we used Th-Cre adult mice to investigate more specifically the involvement of C-LTMRs in at-level pain after thoracic contusion SCI. Using a modified light-dark chamber conditioned place aversion (CPA) paradigm, we assessed chamber preferences and transitions between chambers at baseline, and in response to mechanical and optogenetic stimulation of C-LTMRs. In parallel, at baseline and select post-surgical timepoints, mice underwent non-contact RR recordings and von Frey assessment of mechanical hypersensitivity. The results showed that SCI mice avoided the chamber associated with C-LTMR stimulation, an effect that was more pronounced with optical stimulation. They also displayed elevated RRs at rest and during CPA training sessions. Importantly, these changes were restricted to chronic post-surgery timepoints, when hindpaw mechanical hypersensitivity was also evident. Together, these results suggest that C-LTMR afferent plasticity, coexisting with a potentially facilitatory state of sympathetic activation, drives at-level affective pain following SCI in adult mice. Contribution to the field statementPreclinical studies have only recently sought to understand biological mechanisms connecting peripheral afferent neuron hypersensitivity to pain. Using mechanical brush and selective optogenetic activation in transgenic mice, this study investigated the role of an implicated subpopulation of sensory afferents, the C-low threshold mechanoreceptors (C-LTMRs), that may be transformed from pleasurable touch- to pain-encoding neurons after spinal cord injury (SCI). C-LTMR activation after SCI evoked at-level conditioned pain responses associated with sympathetic activation (increased respiratory rates). These studies provide a foundation for research into the peripheral afferent mechanisms of pain that is expected to translate into new options for pain control in humans.

physiology↗