Biology subjects

Nishino, A. S.

Publications and source records attributed to Nishino, A. S..

4 recordsLinked to original sources

bioRxiv2025

Membranous interacting partners of phage-type plastid RNA polymerase have limited impact on plastid gene expression during chloroplast development

In vascular plants, genes in the plastid genome are transcribed by two types of RNA polymerases, namely bacterial-type nuclear-encoded and phage-type plastid-encoded plastid RNA polymerases (NEP and PEP, respectively). Eudicots, including Arabidopsis, carry two isoforms of NEP, RPOTp and RPOTmp. NEPs transcribe multiple plastid-encoded genes including subunits of PEP and translocon, thus are indispensable for the maintenance of plastids. However, regulatory mechanisms of NEPs are largely unknown. RPOTmp transcribes the 16S rRNA gene from a specific promoter in the seeds during vernalization, and its mutation in Arabidopsis retards the chloroplast development. As interacting partners of RPOTmp, two NEP-INTERACTING PROTEINs (NIP1 and NIP2) have been identified and suggested to suppress RPOTmp activity by tethering RPOTmp to the thylakoid membrane during chloroplast development in the presence of light, but their precise roles in transcriptional regulation remain to be addressed. From these previous reports, we hypothesize that functions of RPOTmp would depend on the light conditions and expression of NIPs. To gain insight into how RPOTmp is controlled, we performed functional analysis of RPOTmp and NIPs using Arabidopsis mutants during germination in the dark and de-etiolation processes under light. We found that RPOTmp-dependent transcription of 16S rRNA is active in imbibed seeds and remains basal level throughout the post-germination processes, regardless of light conditions. We also demonstrated a limited impact of NIPs on RPOTmp function during these processes. Our phylogenetic analysis indicates that NIPs have distinct evolutionary profiles compared to RPOTmp, and Arabidopsis is unlikely to have additional NIP-like proteins in plastids. Based on these findings, we propose a modified model of RPOTmp regulation during chloroplast development: RPOTmp activity remains stable throughout the process of the chloroplast differentiation and is unaffected by light and NIPs.

plant biology↗

bioRxiv2024

Tunicate-specific protein Epi-1 is essential for conferring hydrophilicity to the larval tunic in the ascidian Ciona

Animals must avoid adhesion to objects in the environment to maintain their mobility and independence. The marine invertebrate chordate ascidians are characterized by an acellular matrix tunic enveloping their entire body for protection and swimming. The tunic of ascidian larvae consists of a surface cuticle layer and inner matrix layer. Hydrophilic substances coat the cuticle; this modification is thought to be for preventing adhesion. However, the molecule responsible for regulating this modification has not been clarified. We here found that the tunicate-specific protein Epi-1 is responsible for preventing adhesiveness of the tunic in the ascidian Ciona intestinalis Type A. Ciona mutants with homozygous knockouts of Epi-1 exhibited adhesion to plastic plates and to other individuals. The cuticle of the Epi-1 mutants was fragile, and it lost the glycosaminoglycans supplied by test cells, the accessory cells that normally attach to the tunic surface. Although it has an apparent signal peptide for membrane trafficking, we showed that the Epi-1 protein is localized to the cytosol of the epidermal cells. Our study demonstrated that the emergence of the tunicate-specific protein Epi-1 accelerated the immediate ancestor of tunicates to evolve a tunic by providing a way to avoid the adhesiveness of this structure. HighlightsO_LIThe ascidian Ciona tunic has a glycosaminoglycan (GAG)-coated hydrophilic cuticle. C_LIO_LIEpi-1 is a protein expressed in the epidermis of tunicates during embryogenesis. C_LIO_LIEpi-1 Ciona mutants have GAG-free, hydrophobic and therefore sticky cuticles. C_LIO_LIEpi-1 acquisition may have prevented adhesion of tunicates to environmental objects. C_LI

developmental biology↗

bioRxiv2023

Pulsation waves along the Ciona heart tube reverse by bimodal rhythms expressed by a remote pair of pacemakers

The heart of ascidians, marine invertebrate chordates, exhibits a tubular structure, and heartbeats propagate from one end to the other. The direction of pulsation waves intermittently reverses in the heart of ascidians and their relatives; however, the underlying mechanisms remain unclear. We herein performed a series of experiments to characterize the pacemaker systems in isolated hearts and their fragments and applied a mathematical model to examine the conditions leading to heart reversals. The isolated heart of Ciona sufficiently performed heart reversals, and experimental bisections of isolated hearts revealed that independent pacemakers resided on each side and also that their beating frequencies periodically changed as they expressed bimodal rhythms. Only fragments including 5% or shorter terminal regions of the heart tube maintained autonomous pulsation rhythms, whereas other regions did not. Our mathematical model, based on FitzHugh-Nagumo equations applied to a one-dimensional alignment of cells, demonstrated that the difference between frequencies expressed by the two independent terminal pacemakers determined the direction of propagated waves. Changes in the statuses of the terminal pacemakers between the excitatory and oscillatory modes as well as in their endogenous oscillation frequencies were sufficient to lead to heart reversals. These results suggest that the directions of pulsation waves in the Ciona heart reverse according to the changing rhythms independently expressed by remotely coupled terminal pacemakers. Summary statementPulsation waves traveling along the heart tube of the ascidian Ciona intermittently reverse because of autonomous and periodical changes in beating frequencies at a pair of terminal pacemakers.

physiology↗

bioRxiv2021

An autonomous tail-beating cycle period expressed by a region-specific swimming pattern generator in the Ciona larva

Swimming locomotion in aquatic vertebrates, such as fish and tadpoles, is expressed through orchestrated operations of central pattern generators. These parallel neuronal circuits are ubiquitously distributed and mutually coupled along the spinal cord to express undulation patterns accommodated to efferent and afferent inputs. While such sets of schemes have been shown in vertebrates, the evolutionary origin of those mechanisms along the chordate phylogeny remains unclear. Ascidians, representing a sister group of vertebrates, give rise to tadpole larvae that freely swim in seawater. In this study, we tried to locate the swimming pattern generator in larvae of the ascidian Ciona by examining locomotor ability of segmented body fragments. Our experiments demonstrated necessary and sufficient pattern generator activity in a short region ([~]10% of the body length as the longest estimation) including the trunk-tail junction but excluding most of the trunk and tail with major sensory apparatuses therein. Moreover, we found that these "mid-piece" body fragments express periodic tail beating bursts with [~]20-s intervals without any exogenous stimuli. Comparisons among temporal patterns of tail beating bursts expressed by the mid-piece fragments and by whole larvae placed under different sensory conditions suggested that the presence of parts other than the critical mid-piece had effects to shorten swimming burst intervals, especially in the dark, and also to expand the variance in burst durations. We propose that Ciona larvae perform swimming as modified representations of autonomous and periodic pattern generator drives, which operate locally in the region of the trunk-tail junction. Summary statementMid-piece fragments of tadpole larvae of the ascidian Ciona, lacking most of the anterior trunk and posterior tail, autonomously and periodically express tail beating bursts.

zoology↗