Biology subjects

Niku, M.

Publications and source records attributed to Niku, M..

8 recordsLinked to original sources

bioRxiv2025

Impact of gestational antibiotics on maternal and offspring gut microbiota and growth in pigs

Maternal microbiota modulates the development of the microbiota in the offspring. Effects of gestational antibiotics are not well understood, as most studies have focused on the perinatal period. We treated sows with penicillin, tetracycline or saline on days 78-80 of the 114-119-day gestation, a critical period in the fetal immune system development. Microbiotas were analyzed by 16S rRNA gene amplicon sequencing in sow feces and vagina at days 77 and 113, in colostrum, and in piglet feces at three days, three weeks and ten weeks of age. Sow fecal microbiota changed during pregnancy, but less in antibiotic groups. No significant effects on sow microbiota remained on day 113. The piglets of the antibiotic-treated sows had lower Firmicutes+Actinobacteriota to Bacteroidota+Proteobacteria ratio, lower alpha diversity and higher relative abundance of Escherichia at three days. At ten weeks, they exhibited higher alpha diversity, had higher of Prevotella and Clostridium sp. CAG-127, and smaller increase of Limosilactobacillus than the control. Increased alpha diversity at 10 weeks was associated with lower weight gain during nursing. Oliverpabstia and Mitsuokella were positively associated with growth, while CAG-127 and Campylobacter B were negatively associated. Sow antibiotic treatment decreased the positive effect of Oliverpabstia and Mitsuokella and increased the negative effects of CAG-127 and Campylobacter B. Gestational antibiotics may have had adverse effects on microbiota and growth of the offspring, even if their effects on maternal microbiota were undetectable by parturition.

microbiology↗

bioRxiv2025

Proteomic characterization of extracellular vesicles from 12 commensal bacterial species

Bacterial extracellular vesicles (bEVs) produced by intestinal commensal bacteria mediate host-microbe interactions, but their proteomes have been explored in only a small number of species. We characterized bEVs from in vitro cultures of 12 species of Actinomycetota, Bacillota (Firmicutes), Bacteroidota, Fusobacteria, Pseudomonadota, and Verrucomicrobia. This is the first report of bEVs from Veillonella magna, an exceptional gram-negative Bacillota, and the gram-positives Peptostreptococcus russellii and Turicibacter sanguinis. The morphology and protein subcellular localization patterns of bEVs reflected the envelope structures of their parent bacteria. Notably, V. magna may be able to produce both outer membrane and cytoplasmic membrane vesicles. The proteome compositions were dictated by phylogeny, suggesting largely non-selective packaging of proteins. Annotation of protein functions indicated roles in nutrient metabolism and transport, and in host immune system modulation. Peptidoglycan modifying enzymes and the abundant bacteriophage proteins in Enterobacter cloacae and Limosilactobacillus reuteri bEVs may be involved in vesicle biogenesis. The functions of many abundant bEV proteins are unknown. The most abundant bEV proteins were largely species-specific, but we identified several conserved proteins that may be used as markers to distinguish commensal bEVs from host EVs. Comparison to previous in vitro and fecal metaproteomics data indicates that bEV proteome compositions are reproducible.

microbiology↗

bioRxiv2024

HHV-6B, HHV-7, and B19V Are Frequently Found DNA Viruses in the Human Thymus but Show No Definitive Link with Myasthenia Gravis

Myasthenia gravis (MG) is an autoimmune disorder characterised by autoantibodies that target components of the neuromuscular junction, primarily the acetylcholine receptor (AChR), resulting in muscle weakness. The thymus plays a significant role in MG pathogenesis, particularly in patients under the age of 50, who display pathological alterations and possess elements conducive to autoimmune reactions. Although viral infections are suspected drivers of thymic pathogenesis, the exact aetiology of MG remains elusive. This study investigates the potential link between MG and DNA viruses within the thymus. Using targeted next-generation sequencing and quantitative PCR, we analysed the presence of human parvovirus B19 (B19V) and nine human herpesviruses (HSV-1, HSV-2, VZV, EBV, CMV, HHV-6A, HHV-6B, HHV-7, and HHV-8) in fresh tissue samples from 19 non-thymomatous MG patients, 16 thymomas (3 with and 14 without MG), 41 normal thymus tissues, and 20 tonsils from healthy individuals. HHV-6B was the most common virus, found in over 50% of all tissue groups. B19V DNA was detected in 40% of adult control thymic tissue, 72% of MG thymus, 7.7% of non-MG thymoma, and 50% of tonsil samples. HHV-7 was present in 15-30% of thymus tissues and 95% of tonsils, while EBV was detected in less than 25% of all thymus samples but 85% of tonsils. In B19V seropositive individuals, B19V DNA was detected in 100% of thymic tissue from both MG patients and healthy individuals, except in thymomatous tissues, where it was found in only one of thirteen seropositive individuals. Immunohistochemistry for B19V protein expression did not show evident B19V VP1/VP2 protein expression, indicating dormant viral persistence. Laser capture microdissection (LCM) and RNAscope in situ hybridisation pinpointed B19V DNA localisation to the thymus medulla. This study is the first to demonstrate the persistence of various DNA viruses in the human thymus. However, neither B19V nor the nine human herpesviruses showed specific enrichment in MG thymic tissue compared to controls, suggesting that these viral infections are unlikely to be sole environmental triggers for MG.

immunology↗

bioRxiv2024

Bacterial profiles of the oral, vaginal, and rectal mucosa and colostrum of periparturient sows

The commensal microbiota influences the health, feeding efficiency, and reproductive performance of sows. The microbiota composition in the alimentary and genitourinary tracts and in colostrum/milk during pregnancy and lactation also impacts the developing microbiota and immune system of the piglets and subsequently their growth and health. Knowledge of the microbial compositions is important for evaluation of these effects and for discovering ways to improve the health and productivity of the sows. Oral, vaginal, and rectal mucosa were sampled from 32 sows of variable parity in late pregnancy on four commercial piglet-producing farms in Finland. Colostrum samples were taken within 6 hours of delivery of the first piglet. Microbial compositions were analyzed by 16S rRNA gene amplicon sequencing. Moderate differences in diversity and composition were observed between farms. The most abundant genera of the oral microbiota were Rothia, Moraxella, and Streptococcus. The rectal microbiota was dominated by Clostridium sensu stricto 1. Streptococcus was the most abundant genus in the vagina and colostrum. Differences in relative abundances of genera were detected between primiparous and multiparous sows. Some of these differences were detected across all the farms; in the multiparous sows, the relative abundances of Fusobacterium and Neisseria were lower in oral samples. Clostridium sensu stricto 1, Romboutsia, and Lachnospiraceae_UCG_007 were higher and Prevotella lower in rectal samples, and Streptococcus higher in colostrum samples. In vaginal samples, approximately half of the multiparous sows had significantly higher relative abundances of the genera Fusobacterium and Streptococcus than the primiparous sows. Among the differentially abundant taxa, F. necrophorum and F. nucleatum were identified in oral samples, Fusobacterium gastrosuis and Fusobacterium necrophorum in vaginal samples, and Streptococcus dysgalactiae in colostrum samples. Most of the differences were due to unidentified species within the respective genus. This study provides a comprehensive overview of the mucosal and colostrum microbiota of periparturient sows during normal production conditions on Finnish commercial farms, including potentially interesting differences in the relative abundances of several genera at different mucosal sites between sows of low and high parity.

microbiology↗

bioRxiv2022

Impacts of maternal microbiota and microbial metabolites on fetal intestine, brain and placenta

The maternal microbiota modulates fetal development, but the mechanisms of these earliest host-microbe interactions are unclear. We compared full-term fetuses from germ-free (GF) and normally colonized mouse dams by gene expression profiling and non-targeted metabolomics. The developing immune system was strongly dependent on the maternal microbial status. In the fetal intestine, critical components mediating host-microbe interactions were differentially expressed. In fetal brain and placenta, interferon and inflammatory signaling were downregulated in germ-free fetuses. Neural system development and function, translation and RNA metabolism, and regulation of energy metabolism were significantly affected at the gene expression level. These impacts were strongly associated with microbial metabolite concentrations in the fetal tissues, suggesting that they are largely, although perhaps not exclusively mediated by maternal microbial metabolites absorbed through placenta. Several aryl sulfates were among the compounds strongly associated with gene expression differences. The germ-free fetus may suffer from depletion of queuine, a bacterial hypermodified nucleobase essential for eukaryotic tRNA stability and function.

microbiology↗

bioRxiv2020

The composition of the microbiota in the full-term fetal gut and amniotic fluid: a bovine caesarean section study

The fetal development of the intestinal immune system is stimulated by the maternal microbiota, but it is still unclear whether viable bacteria exist in the healthy fetus. Analysis of such low microbial biomass environments are challenging due to contamination issues. The aims of the current study were to assess the bacterial load and characterize the bacterial composition of the amniotic fluid and meconium of full-term calves, leading to a better knowledge of prenatal bacterial seeding of the fetal intestine. Amniotic fluid and rectal meconium samples were collected during and immediately after elective caesarean section, performed in 25 Belgian Blue cow-calf couples. The samples were analyzed by qPCR, bacterial culture using GAM agar and 16S rRNA gene amplicon sequencing. To minimize the effects of contaminants, we included multiple technical controls and stringently filtered the 16S rRNA gene sequencing data to exclude putative contaminant sequences. The meconium samples contained a significantly higher amount of bacterial DNA than the negative controls and 5 of 24 samples contained culturable bacteria. In the amniotic fluid, the amount of bacterial DNA was not significantly different from the negative controls and all samples were culture negative. Bacterial sequences were identified in both sample types and were primarily of phyla Proteobacteria, Firmicutes, Bacteroidetes and Actinobacteria, with some individual variation. We conclude that most calves encounter in utero maternal-fetal transmission of bacterial DNA, but the amount of bacterial DNA is low and viable bacteria are rare.

microbiology↗

bioRxiv2020

Metabolomic signature of the maternal microbiota in the fetus

The maternal microbiota affects the development of the offspring by microbial metabolites translocating to the fetus. We investigated samples of placenta, fetal intestine and brain from germ-free (GF) and specific pathogen free (SPF) mouse dams by non-targeted metabolic profiling. One hundred one annotated metabolites and altogether 3680 molecular features were present in significantly different amounts in the placenta and/or fetal organs of GF and SPF mice. The concentrations of more than half of the annotated and differentially expressed metabolites were lower in the GF organs, suggesting their microbial origin or a metabolic response of the host to the presence of gut microbiota. The clearest separation was observed in the placenta. Metabolites that were detected in lower amounts in the fetal organs in the GF mice included 5-aminovaleric acid betaine, trimethylamine N-oxide, catechol-O-sulphate, hippuric and pipecolic acid. Derivatives of the amino acid tryptophan, such as kynurenine, 3-indolepropionic acid and hydroxyindoleacetic acid, were also decreased in the absence of microbiota. Several metabolites had higher levels in the GF mice. These could be precursors of microbial metabolites or indicators of host metabolic response to the absence of gut microbiota. Ninety-nine molecular features were only detected in the SPF mice, suggesting the existence of yet unidentified microbially modified metabolites that potentially influence fetal development.

microbiology↗

bioRxiv2019

The composition of the perinatal intestinal microbiota in horse

The establishment of the intestinal microbiota is critical for the digestive and immune systems. We studied the early development of the microbiota in horse, a hindgut fermenter, from birth until 7 days of age, by qPCR and 16S rRNA gene amplicon sequencing. To evaluate initial sources of foal microbiota, we characterized dam fecal, vaginal and oral microbiotas. We utilised an amplicon sequence variant (ASV) based pipeline to maximize resolution and reproducibility. Stringent ASV filtering based on prevalence and abundance in samples and controls purged reagent contaminants while preserving intestinal taxa. The newborn rectum contained small amounts of diverse bacterial DNA, with a profile closer to mare feces and vagina than mouth. 24 hours after birth, the intestine was colonized by Firmicutes and Proteobacteria, some foals dominated by a single genus. At day 7, the phylum-level composition resembled adult feces but genera were different. The mare vaginal microbiota contributed to 24 h and 7 day microbiotas. It contained few lactobacilli, with Corynebacterium, Porphyromonas, Campylobacter and Helcococcus as the most abundant genera. In the oral mucosa, Gemella was extremely abundant. Our observations suggest that bacteria or bacterial components translocate to the equine fetus, but the intestinal microbiota changes rapidly after birth.

microbiology↗