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Neufeld, D.

Publications and source records attributed to Neufeld, D..

2 recordsLinked to original sources

Headwaters to Valley: Water quality in rivers transitioning from forest to agricultural bottomland

Many waterways flow out of forestlands, which tend to maintain higher water quality, into agricultural lands, which tend to degrade water quality. The roles of land cover in impacting key water quality parameters (phosphorus, nitrogen, total suspended solids, bacteria, and conductivity) were investigated for the watershed of the North and South Fork of the Shenandoah River, Virginia. This area has a particularly sharp boundary between heavily forested and heavily agricultural regions. Two datasets were analyzed: 1) a large number of datapoints spanning a 20-year range in the Water Quality Portal (WQP) database, and 2) transects along three representative rivers systems over the span of a 4-year period. All parameters trended better in forested regions than agricultural regions. This was particularly true for nitrogen and conductivity; phosphorus, TSS and bacteria showed more local variability, especially in the agricultural region. Periods of high flow increased phosphorus, sediment and bacteria concentrations, and decreased conductivity, but not when drainage basin forest cover was less than 80%. Transects showed that waterways flowing out of forestland maintained higher water quality for approximately the first 8 km in agricultural land. Both transect and WQP data indicated higher water quality when the percent of forested land cover in a drainage basin was about 70-80%. Thus, forestland does mitigate the impacts of agriculture on water quality to some degree, but this effect rapidly diminishes as forest cover of the watershed lessens. Furthermore, forests themselves have degraded water quality at certain times and places; for instance, nutrients level were in the medium to high stress level for aquatic life in approximately 15% of samples. This study illustrates general trends of land cover effects on water quality, while also highlighting both site-specific variability, and the dynamics of water quality as water flows out of forested areas into agricultural areas.

ecology↗

USP1 deubiquitinates protein kinase Akt to inhibit PI3K-Akt-FoxO signaling

PI3K-Akt-FoxO-mTOR signaling is the central pathway controlling growth and metabolism in all cells. Activation of this pathway requires ubiquitination of Akt prior to its activation by phosphorylation. Here, we found that the deubiquitinating (DUB) enzyme USP1 removes K63-linked polyubiquitin chains on Akt to sustain PI3K-Akt-FoxO signaling low during prolonged starvation. DUB screening platform identified USP1 as a direct DUB for Akt, and USP1 depletion in atrophying muscle increased Akt ubiquitination, PI3K-Akt-FoxO signaling, and glucose uptake during fasting. Co-immunoprecipitation and mass spectrometry identified Disabled-2 (Dab2) and the tuberous sclerosis complex TSC1/TSC2 as USP1 bound proteins. During starvation, Dab2 was essential for Akt recruitment to USP1/UAF1 complex, and for PI3K-Akt-FoxO inhibition. Additionally, to maintain its own protein levels high, USP1 limits TSC1 levels to sustain mTOR-mediated basal protein synthesis rates. This USP1-mediated suppression of PI3K-Akt-FoxO signaling probably contributes to insulin resistance in catabolic diseases and perhaps to malignancies seen with USP1 mutations.

cell biology↗