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Nematollahi, L.

Publications and source records attributed to Nematollahi, L..

2 recordsLinked to original sources

GROQ-seq Datasets Across Transcription Factors (LacI, RamR, VanR), T7 RNA Polymerase and TEV Protease

Predicting any proteins function from its sequence alone would be a significant breakthrough in molecular biology. Although machine learning approaches have sought to tackle this, their limited generalizability reflects the absence of sufficiently large, open, diverse, and unified datasets. To address this data gap, we developed a high-throughput experimental platform called GROQ-seq (Growth-based Quantitative Sequencing). In GROQ-seq, a proteins function can be linked to a sequencing-based readout that enables scalable characterization of large variant libraries in Escherichia coli. Here, we present pilot datasets demonstrating its performance across three distinct protein function classes: transcription factors, polymerases, and proteases. The objective of this report is to present the datasets and to provide users with a clear and transparent characterization of their properties, including both the strengths and limitations.

bioengineering↗

Continuous evolution of a halogenase enzyme with improved solubility and activity for sustainable bioproduction

Halogenation enhances the stability and function of pharmaceuticals, biomaterials, and industrial compounds. However, chemical halogenation lacks stereoselectivity and requires the use of toxic or expensive chemicals. Although enzymatic halogenation can improve selectivity and reduce environmental impact, current halogenases are inefficient and insoluble, leading to low yields that limit their applications. Here, we develop RebHEvo4, a soluble and highly active tryptophan halogenase, containing 12 mutations that confer 37-fold and 44-fold increases in 7-chloro and 7-bromotryptophan production respectively, in vivo. To create RebHEvo4, we devised an aminoacyl tRNA synthetase based halogenase biosensor and conducted over 500 hours of phage-assisted continuous evolution (PACE). Use of RebHEvo4 in a bioreactor resulted in the production of 2.7 g/L of halogenated tryptophan. When coupled with a downstream enzyme, RebHEvo4 allowed 36-fold increased yields of halogenated tryptamines compared to the wild-type enzyme. Additionally, RebHEvo4 enabled efficient production of genetically encoded antimicrobial halogenated peptides. The efficient, site-specific halogenation by our evolved halogenase will accelerate sustainable biomanufacturing of halogenated drugs.

biochemistry↗