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Neethiraj, R.

Publications and source records attributed to Neethiraj, R..

3 recordsLinked to original sources

Recommendations for Bioinformatics in Clinical Practice

Next Generation Sequencing (NGS) is increasingly used in clinical diagnostics, largely driven by the success and robustness of Whole Genome Sequencing (WGS). Whereas updated guidelines exist for how to interpret and report on variants that are identified from NGS using bioinformatics pipelines, there is a need for standardised bioinformatics practices for diagnostics to ensure clinical consensus, accuracy, reproducibility and comparability of the results. This article presents consensus recommendations developed by 13 clinical bioinformatics units taking part in the Nordic Alliance for Clinical Genomics (NACG), by expert bioinformaticians working in clinical production. The recommendations are based on clinical practice and focus on analysis types, test and validation, standardisation and accreditation, as well as core competencies and technical management required for clinical bioinformatics operations. Key recommendations include adopting the hg38 genome build as the reference and a standard set of recommended analyses, including the use of multiple tools for structural variant (SV) calling and in-house data sets for filtering recurrent calls. Clinical bioinformatics production should operate under the ISO 15189 standard, utilising off-grid clinical-grade high-performance computing systems, standardised file formats, and strict code version control. Containerized software containers or environment management systems are needed to ensure reproducibility. Pipelines should be rigorously documented and tested for accuracy and reproducibility, minimally covering unit, integration, and end-to-end testing. Standard truth sets such as GIAB and SEQC2 for germline and somatic variant calling, respectively, should be supplemented by recall testing of previously validated clinical cases. Data integrity must be verified using file hashing, and sample identity should be checked via sample fingerprinting and genetically inferred identification markers such as sex and relatedness. Finally, clinical bioinformatics teams should encompass diverse skills, including software development, data management, quality assurance, and domain expertise in human genetics. These recommendations provide a consensus framework for standardising bioinformatics practices across clinical WGS applications and can serve as a practical guide to facilities that are new to large-scale sequencing-based diagnostics, or as a reference for those who already run high-volume clinical production using NGS.

bioinformatics↗

A female alternative life-history strategy arose via novel recruitment of homeobox gene, BarH-1

Tradeoffs affect resource allocation during development and result in fitness consequences that drive the evolution of life history strategies. Yet despite their importance, we know little about the mechanisms underlying life history tradeoffs in wild populations. Many species of Colias butterflies exhibit an alternative life history strategy (ALHS) where females divert resources from wing pigment synthesis to reproductive and somatic development. Due to this reallocation, a wing color polymorphism is associated with the ALHS: individuals have either yellow/orange or white wings. Here we map the genetic basis of the ALHS switch in Colias crocea to a transposable element insertion downstream of the Colias homolog of BarH-1, a homeobox transcription factor. Using CRISPR/Cas9 gene editing, antibody staining, and electron microscopy we find morph-specific specific expression of BarH-1 suppresses the formation of pigment granules in wing scales. Lipid and transcriptome analyses reveal physiological differences associated with the ALHS. These findings characterize a novel mechanism for a female-limited ALHS and show that the switch arises via recruitment of a transcription factor previously known for its function in cell fate determination in pigment cells of the retina.

genomics↗

A butterfly chromonome reveals selection dynamics during extensive and cryptic chromosomal reshuffling

Chromosome evolution, an important component of mico- and macroevolutionary dynamics 1-5, presents an enigma in the mega-diverse Lepidoptera6. While most species exhibit constrained chromosome evolution, with nearly identical haploid chromosome counts and chromosome-level shared gene content and collinearity among species despite more than 140 Million years of divergence7, a small fraction of species independently exhibit dramatic changes in chromosomal count due to extensive fission and fusion events that are facilitated by their holocentric chromosomes7-9. Here we address this enigma of simultaneous conservation and dynamism in chromosome evolution in our analysis of the chromonome (chromosome level assembly10) of the green-veined white butterfly, Pieris napi (Pieridae, Linnaeus, 1758). We report an unprecedented reorganization of the standard Lepidopteran chromosome structure via more than 90 fission and fusion events that are cryptic at other scales, as the haploid chromosome number is identical to related genera and gene collinearity within the large rearranged segments matches other Lepidoptera. Furthermore, these rearranged segments are significantly enriched for clusters of functionally related genes and the maintenance of ancient telomeric ends. These results suggest an unexpected role for selection in shaping chromosomal evolution when the structural constraints of monocentricq chromosomes are relaxed.

evolutionary biology↗