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Biology subjects

Neelakantan, A.

Publications and source records attributed to Neelakantan, A..

2 recordsLinked to original sources

Prioritizing landscapes to reconcile biodiversity conservation, ecosystem services, and human well-being in India

Biodiversity conservation and human well-being are tightly interlinked; yet mismatches in the scale at which both priorities are planned and implemented have exacerbated biodiversity loss, erosion of ecosystem services, and declining human quality of life. India houses the second largest human population on the planet, while <5% of the countrys land area is effectively protected for conservation. This warrants landscape-level conservation planning through a judicious mix of land-sharing and land-sparing approaches, and co-production of ecosystem services. Through a multi-faceted assessment, we prioritize spatial extents of land parcels that, in the face of anthropogenic threats, can safeguard conservation landscapes across Indias biogeographic zones. We find that only a fraction (~15%) of such priority areas identified here are encompassed under Indias extant PA network, and several landscapes of high importance were omitted in all previous global-scale assessments. We then examined the spatial congruence of priority areas with administrative units earmarked for economic development by the Indian government, and propose management-zoning through state-driven and participatory approaches. Our spatially explicit insights can help meet the twin goals of biodiversity conservation and sustainable development in India and other countries across the Global South.

ecology↗

Characterization of α 3 Glycine Receptors with Ginkgolide B and Picrotoxin

Ginkgolide B (GB) and picrotoxin (PTX) are antagonists of the major inhibitory receptors of the central nervous system: GABA and glycine receptors (GlyRs). GlyRs contain one or more of the four alpha subunit isoforms of which 1 and 2 have been extensively studied. This report compares GB and PTX block of 3 GlyRs expressed in HEK 293 cells, using whole-cell patch clamp techniques. In CNS, 3 exists as a heteropentamer in conjunction with beta subunits in a 2:3{beta} ratio. Thus, the nature of block was also tested in 3{beta} heteromeric glycine receptors. GB and PTX blocked 3 GlyRs both in the presence (liganded state) and absence of glycine (unliganded state). This property is unique to 3 subunits; 1 and 2 subunits are only blocked in the liganded state. The GB block of 3 GlyRs is voltage-dependent (more effective when the cell is depolarized) and non-competitive, while the PTX block is competitive and not voltage-dependent. The heteromeric and homomeric 3 GlyRs recovered significantly faster from unliganded GB block compared to liganded GB block, but no such distinction was found for PTX block suggesting more than one binding site for GB. This study sheds light on features of the 3 GlyR that distinguish it from the more widely studied 1 and 2 subunits. Understanding these properties can help decipher the physiological functioning of GlyRs in the CNS and may permit development of subunit specific drugs.

neuroscience↗