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Nakarakanti, N. K.

Publications and source records attributed to Nakarakanti, N. K..

2 recordsLinked to original sources

HMMR/RHAMM recruits SACK1D/FAM83D-CK1α complex at the mitotic spindle to control spindle alignment

The SACK1D/FAM83D-CK1 complex assembles at the mitotic spindle to orchestrate proper spindle positioning and error-free progression through mitosis. The full molecular picture of how this complex assembles and disassembles over the cell division cycle remains to be fully defined. Here, we show that HMMR is critical for SACK1D-CK1 complex formation at the spindle, co-localizes with the SACK1D-CK1 complex throughout mitosis, and is necessary for correct mitotic spindle alignment. We find that HMMR binds to the C-terminal -helix of SACK1D, and this helix is also important for the mitotic interaction between SACK1D and CK1. We demonstrate that HMMR binding stabilizes SACK1D. We map the mitotic hyperphosphorylation sites on SACK1D and show that this hyperphosphorylation signals the destruction of SACK1D upon mitotic exit. The destruction also requires the C-terminal -helix of SACK1D, suggesting that hyperphosphorylation of SACK1D in mitosis potentially exposes the C-terminal degron sequences resident on SACK1D. This study provides key molecular insights into the assembly and fate of the HMMR-SACK1D-CK1 complex at the mitotic spindle.

cell biology↗

UHRF1-SRA recognizes symmetric non-CG methylated DNA through dual-flip out of 5-methyl cytosines

Non-CG DNA methylation (non-mCG) is enriched in the genome of brain neurons and germline cells. Non-mCG is differentially distributed on neuronal X-chromosome in males and females. Accumulation of non-mCG during postnatal brain development correlates with reduced gene expression and inactivation of distal regulatory elements, and allele specific gene regulation. Recently, UHRF1 has been found to contribute to de novo non-CG methylation, however, whether UHRF1 could recognize non-mCG is not known. Here, we have demonstrated through calorimetric measurements that the SRA domain of UHRF1 can recognize mCH and fully-mCHG, types of non-mCG. Furthermore, our ITC binding analyses with methylated CG DNA (mCG) revealed 6-fold decrease in binding affinity for fully-mCG compared to hemi-mCG and, despite symmetrical 5mCs, stoichiometry of 1:1 for UHRF1 SRA binding to fully-mCG indicates UHRF1 may not form stable complex with fully-mCG DNA. In contrast, UHRF1 SRA recognizes fully-mCHG with a stoichiometry of 2:1 protein to DNA duplex, and has tighter binding compared to fully-mCG. Crystal structure of UHRF1 SRA bound to 5mC containing DNA in fully-mCHG context revealed dual flip-out mechanism of 5mC recognition. Altogether, this study indicates that UHRF1 SRA also recognizes non-mCG DNA, besides known hemi-mCG DNA and exhibits contrasting mechanisms for hemi-mCG and fully-mCHG DNA recognition. These findings may open a new window to investigate the biological function of non-CG methylation recognition by the UHRF1.

biophysics↗