Biology subjects

Najafian, K.

Publications and source records attributed to Najafian, K..

3 recordsLinked to original sources

bioRxiv2025

The Global Wheat Full Semantic Organ Segmentation (GWFSS) dataset

Computer vision is increasingly used in farmers fields and agricultural experiments to quantify important traits. Imaging setups with a sub-millimetre ground sampling distance enable the detection and tracking of plant features, including size, shape, and colour. Although todays AI-driven foundation models segment almost any object in an image, they still fail for complex plant canopies. To improve model performance, the global wheat dataset consortium assembled a diverse set of images from experiments around the globe. After the head detection dataset (GWHD), the new dataset targets a full semantic segmentation (GWFSS) of wheat organs (leaves, stems and spikes) covering all developmental stages. Images were collected by 11 institutions using a wide range of imaging setups. Two datasets are provided: i) a set of 1096 diverse images in which all organs were labelled at the pixel level, and (ii) a dataset of 52,078 images without annotations available for additional training. The labelled set was used to train segmentation models based on DeepLabV3Plus and Segformer. Our Segformer model performed slightly better than DeepLabV3Plus with a mIOU for leaves and spikes of ca. 90%. However, the precision for stems with 54% was rather lower. The major advantages over published models are: i) the exclusion of weeds from the wheat canopy, ii) the detection of all wheat features including necrotic and senescent tissues and its separation from crop residues. This facilitates further development in classifying healthy vs. unhealthy tissue to address the increasing need for accurate quantification of senescence and diseases in wheat canopies.

plant biology↗

bioRxiv2022

Predicting Phenotypes From Novel Genomic Markers Using Deep Learning

Genomic selection models use Single Nucleotide Polymorphism (SNP) markers to predict phenotypes. However, these predictive models face challenges due to the high dimensionality of genome-wide SNP marker data. Thanks to recent breakthroughs in DNA sequencing and decreased sequencing cost, the study of novel genomic variants such as Structural Variations (SVs) and Transposable Elements (TEs) become increasingly prevalent. In this paper, we develop a deep convolutional neural network model, NovGMDeep, to predict phenotypes using SVs and TEs markers for genomic selection. The proposed model is trained and tested on samples of A. thaliana and O. sativa using k-fold cross-validation. The prediction accuracy is evaluated using Pearsons Correlation Coefficient (PCC), Mean Absolute Error (MAE), and Standard Deviation (SD) of MAE. The predicted results showed higher correlation when the model is trained with SVs and TEs than with SNPs. NovGMDeep also has higher prediction accuracy when comparing with conventional statistical models. This work sheds light on the unrecognized function of SVs and TEs in genotype-to-phenotype associations, as well as their extensive significance and value in crop development.

bioinformatics↗

bioRxiv2022

Semi-Self-Supervised Learning for Semantic Segmentation in Images with Dense Patterns

Deep learning has shown potential in domains where large-scale annotated datasets are available. However, manual annotation is expensive, time-consuming, and tedious. Pixel-level annotations are particularly costly for semantic segmentation in images with dense irregular patterns of object instances, such as in plant images. In this work, we propose a method for developing high-performing deep learning models for semantic segmentation of wheat heads utilizing little manual annotation. We simulate a computationally-annotated dataset using a few annotated images, a short unannotated video clip of a wheat field, and several video clips from fields with no wheat. This dataset is then used to train a customized U-Net model for wheat head segmentation. Considering the distribution shift between the simulated and real data, we apply three domain adaptation steps to gradually bridge the domain gap. Only using two annotated images, we achieved a Dice score of 0.89 on the internal test set, i.e., images extracted from the wheat field video. The model trained using only two annotated images was evaluated on a diverse external dataset collected from 18 different domains across five countries and achieved a Dice score of 0.73. To further expose the model to images from different growth stages and environmental conditions, we incorporated two annotated images from each of the 18 domains and further fine-tuned the model. This resulted in improving the Dice score to 0.91. These promising results highlight the utility of the proposed approach in the absence of large-annotated datasets. Although the utility of the proposed method is shown on a wheat head dataset, it can be extended to other segmentation tasks with similar characteristics of irregularly repeating patterns of object instances.

plant biology↗