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Nagrath, A.

Publications and source records attributed to Nagrath, A..

2 recordsLinked to original sources

Multistage AI Crop Inspection and Segmentation Framework (MACISF)

Crop quality assessment is one of the most critical components of modern agriculture, which directly influences yield optimization, post-harvest management, and food security. Conventional evaluation of field crops relies on manual inspection, which is time-consuming, subjective, and unscalable. This paper proposes the Multistage AI Crop Inspection and Segmentation Framework (MACISF), an automated multistage deep learning pipeline for fruit quality evaluation from visual features. The pipeline is comprised of three stages: (i) an EfficientNetV2-L backbone, fine-tuned with a three-stage progressive strategy, for crop type classification; (ii) seven species-specific EfficientNetV2-S classifiers for healthy-versusdefective detection; and (iii) a YOLOv8m module for defect localization. Trained and evaluated on a composite dataset spanning seven fruit species, MACISF achieves 99.23% crop type accuracy (macro F1-score 99.23%, macro AUC-ROC 0.993), 99.39% mean health state accuracy with 99.51% mean defective-class recall, and a projected grading mAP@0.5 of 92.3%. On crop type classification, the Stage 1 classifier is second only to InceptionV3 (by 0.15 percentage points) among seven models trained on the same data, and outperforms ResNet-50, Xception, EfficientNetB3, DenseNet-121, and MobileNetV2. Unlike these singletask baseline models, MACISF additionally delivers health-state assessment, defect severity grading, and a per-unit economic and nutritional evaluation. Results are reported on heldout test data from a controlled dataset, and field validation is left for future work.

plant biology↗

CLIC-dependent internalization of caveolin-1 to lysosomal vacuoles in response to osmotic regulation

Originally thought to be a major endocytic portal, caveolae are now considered to function as a membrane buffer, whereby caveolae flattening protects the plasma membrane from rupture under mechanical stress, such as hypotonic shock. However, the fate of the caveolae coat protein caveolin-1 in response to hypotonic shock is not known. Here, we show that extended hypotonic shock induces ubiquitin-independent, CLIC-dependent endocytosis of caveolin-1 to large, intracellular, CD44-positive, pH-neutral lysosomal vacuoles negative for multivesicular body markers. Caveolin-1 recycles from these vacuoles to the plasma membrane upon return to isotonic conditions. Caveolin-1 internalizes not in response to initial cell expansion upon hypotonic shock but rather subsequent reduction of cell volume, and also in low-tension cells grown on reduced stiffness hydrogels. Upon hypertonic shock, caveolin-1 internalization occurs selectively in PC3 cells, lacking cavin-1, required for caveolae formation, and is inhibited upon cavin-1 reintroduction. CLIC endocytosis of non-caveolar caveolin-1 to neutral pH lysosomal vacuoles in response to osmotic regulation defines a novel recycling pathway enabling caveolae membrane buffering.

cell biology↗