Biology subjects

Naderi, M.

Publications and source records attributed to Naderi, M..

4 recordsLinked to original sources

bioRxiv2026

Therapeutic Potential of the Novel Monoclonal Antibody 7-4G against HBsAg in a Mouse Model of Chronic Hepatitis B Virus Infection

Chronic hepatitis B virus (HBV) infection remains a major global health challenge because current antiviral therapies rarely achieve complete viral clearance. We evaluated the antiviral activity of a newly developed monoclonal antibody, 7-4G mAb, directed against the hepatitis B surface antigen (HBsAg). The antibody showed specific binding to the p27 component of HBsAg and efficiently removed HBV-related particles from patient serum in vitro. Significant HBV neutralizing activity of 7-4G mAb was observed in primary human hepatocytes and in humanized mouse models. In persistently HBV-infected PXB mice, a single administration of 7-4G mAb, either alone or in combination with another monoclonal antibody, reduced serum HBsAg levels and HBV DNA levels by two orders magnitude for two weeks after the treatment. In half of the treated mice, suppression of both viral markers was maintained through the study period, whereas rebound occurred within three to four weeks in the remaining animals. In addition, 7-4G mAb enhanced the HBV DNA-lowering effect of entecavir, a known anti-HBV drug. These findings support the further development of 7-4G mAb as part of an innovative HBV treatment strategy, addressing limitations of existing therapies and advancing toward a more effective HBV management solution.

immunology↗

bioRxiv2026

Misleading Success: Genomes Reveal Critical Risks to European Gray Wolves

Have European gray wolves recovered? Despite an increase to [~]21,000 wolves (Canis lupus), our genomic analyses reveal significant risks to their long-term viability. We analyzed over 200 whole-genomes spanning five major European populations. Rather than a single recovering population, European wolves form a mosaic of isolated, independently evolving lineages, mostly diverging in the late Pleistocene. All lineages have contemporary effective population sizes below the threshold for long-term viability (Ne [≥] 500) and show extensive inbreeding. Runs of homozygosity reveal population-specific inbreeding histories spanning recent to deep timeframes. Most lineages exhibit higher realized than masked genetic load, indicating emerging inbreeding depression. These findings challenge claims that downlisting European wolves is biologically warranted: none of these populations currently meets thresholds associated with favorable conservation status.

evolutionary biology↗

bioRxiv2025

A non-invasive approach to awake mouse fMRI compatible with multi-modal techniques

Mouse functional magnetic resonance imaging (fMRI) studies contribute significantly to basic fundamental and translational neuroscience research. Performing fMRI in awake mice could facilitate complex tasks in the magnet and improve translational validity by avoiding anesthesia-related neural and neurovascular changes. Existing surgical approaches provide excellent motion control but are not desirable for all experiments aiming to scan awake mice. These include studies with transgenic mouse lines that are vulnerable to anesthesia or mice in longitudinal studies involving cognition. To address these needs, we propose a non-invasive restraint to scan mice in the awake state. The restraint was designed to be compatible with brain stimulation and recording approaches often combined with fMRI. It was evaluated on the basis of motion, fMRI data quality, and animal stress levels, and compared to a conventional headpost restraint. We found the proposed approach was effective at restraining mice across a broad range of weights without the need for any anesthesia for setup. The non-invasive restraint led to higher data attrition after censoring high motion volumes, but by acquiring roughly 25% more data we could obtain comparable network spatial specificity to the headpost approach. Our results demonstrate a simple open-source head restraint that can be used for awake mouse fMRI for certain cohorts, and we establish suitable acclimation and scanning protocols for use with this restraint.

neuroscience↗

bioRxiv2023

Human-brown bear conflicts in Turkey are driven by increased human presence around protected areas

Human-wildlife conflict is increasing steadily worldwide and is rapidly becoming an important challenge for the success of conservation programs. Brown bears, which suffer from reduced habitat suitability and quality globally, frequently conflict with humans. These animals need large home ranges to fulfill their habitat requirements. When space and food are restricted, brown bears frequently shift towards human-dominated landscapes that offer reliable food sources. As a country where most of the landscape and habitats are human-dominated, human-brown bear conflict events (HBCs) are frequent in Turkiye. However, there has been no formal analysis of the nature and scope of these conflicts at the country level. Here, using HBC data from 2017 to 2022, we determined the spatial and temporal dynamics of HBC events and generated a risk probability map based on anthropogenic predictors and geographic profiling, to determine the factors driving HBC across Turkiye. HBC events did not show any annual or seasonal trends but varied considerably across biogeographic regions, with most conflicts occurring along the Black Sea coast and Eastern Anatolia. Sixty percent of all conflicts were due to bear foraging behavior in human settlements while twelve percent were the result of human activity in forests, with 57% of all conflict events resulting in direct injury to either humans or bears. We found that distance to villages, distance to protected areas, distance to farmland and human footprint to be the most important factors contributing to conflict risk. Consequently, 21% of the country was found to be under human-bear conflict risk, with 43% of the risks occurring within a 10 km radius from the centers of protected areas. Our analyses indicate that the high occurrence of HBCs is mainly the result of limited natural areas and resources available to brown bears and the increasing human encroachment in and around core bear habitats.

ecology↗