Murugesan, s. | bioRxiv Science

bioRxiv2020

Effect of organic zinc supplementation in hens on fertility from cryopreserved semen

Organic zinc supplementation in hen has been reported to improve fertility. The current study evaluated the effect of organic zinc supplementation in hens on fertility after insemination with cryopreserved semen. White Leghorn rooster semen was cryopreserved using 4% dimethylsulfoxide (DMSO) in 0.5ml French straws. Different semen parameters and fertility were assessed in post-thaw samples. White Leghorn hens were divided into 5 groups with 30 birds in each group. Each group was further divided into six replicates of five birds each. The control group was fed basal diet, other groups were fed with basal diet supplemented with 40, 60, 120 and 160 mg/kg organic zinc (zinc proteinate). After two weeks of feeding insemination was done in hens per vagina using thawed semen (200 million sperm/0.1 ml). Basal group hens were inseminated with fresh or cryopreserved semen and served as control groups. Sperm motility, live sperm, and acrosome intact sperm parameters were significantly (p < 0.05) lower in post-thaw semen samples. Fertility from cryopreserved semen was significantly (p < 0.05) lower and organic zinc supplemented hens had fertility similar to that of cryopreserved semen inseminated into basal diet group hens. In conclusion, organic zinc supplementation in hens does not improve fertility after insemination with 4% DMSO cryopreserved semen.Competing Interest StatementThe authors have declared no competing interest.View Full Text

physiology↗

bioRxiv2020

Cryopreservation of Ghagus chicken semen: effect of cryoprotectants diluents and thawing temperature

The present study evaluated the effects of cryoprotectants, semen diluents and thawing temperature during Ghagus chicken semen cryopreservation. Four different experiments were conducted; Experiment 1- semen was cryopreserved using 6% dimethylacetamide (DMA) and 2% dimethylsulfoxide (DMSO) in Sasaki diluent (SD) and Lake and Ravie diluent (LRD), Experiment 2 and 3- semen was cryopreserved using 8% Ethylene Glycol (EG) in SD, LRD and Red Fowl Extender (RFE), Experiment 4- semen was cryopreserved using 6% dimethylformamide (DMF) in SD, LRD and Beltsville Poultry Semen Extender (BPSE). Semen was cryopreserved in 0.5 ml French straws. Thawing was done at 5{degrees}C for 100 sec in ice water in Experiments 1, 2 and 4, whereas in Experiment 3 thawing was done at 37{degrees}C for 30 sec. The post-thaw sperm motility, live sperm and percent acrosome intact sperm were significantly (P<0.05) lower in cryopreserved samples in all the experiments. No fertile eggs were obtained from cryopreserved samples in Experiments 1 and 2, except for 8% EG RFE treatment where the fertility was 0.83%. In Experiments 3 and 4, highest fertility was obtained in LRD treatment 48.12 and 30.89% respectively. In conclusion, using cryoprotectant EG (8%) and thawing at 37{degrees}C for 30 sec, and DMF (6%) resulted in acceptable level of fertility in Ghagus chicken. Though the diluents influenced post-thaw in vitro semen parameters the fertility was not affected. In addition, results indicated that thawing temperature may be a critical stage in the cryopreservation protocol.

physiology↗