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Mukherjee, S. K.

Publications and source records attributed to Mukherjee, S. K..

3 recordsLinked to original sources

Kinetics of local C3 production orchestrates neutrophil recruitment in lung injury

Complement component 3 (C3) is crucial for host defense against bacteria. While the liver is the primary source of circulating C3, local C3 production at barrier surfaces such as the lung is key in early responses. Yet, how local complement-mediated responses are initiated at mucosal barriers is unknown. This study investigates the kinetics and necessity of lung-derived C3 during the initial hours of an infection. Using models of bacterial pneumonia in ex vivo-perfused human lungs and mice deficient in liver-derived C3, we demonstrate that intrapulmonary C3 production and activation precedes the accumulation of circulating C3 into the bronchoalveolar space. Utilizing mice deficient in lung-derived C3, we demonstrate that epithelial cell-derived C3 is required for early neutrophil recruitment in pneumonia. Transcriptomic and proteomic analyses reveal that neutrophil chemotactic pathways such as C5a and CXCL2 depend on lung epithelial cell-derived C3. These findings demonstrate how lung epithelial-derived C3 influences early mucosal responses to infection via both canonical (direct) and non-canonical (indirect) pathways. SUMMARYAlburquerque et al show an initial, entirely local phase of complement-mediated mucosal protection before a subsequent, systemic response occurs in the setting of barrier disruption. Their work suggests that complement component C3 derived locally at a barrier from the epithelium influences early responses to infection by recruiting neutrophils via multiple pathways independent of circulating C3.

immunology↗

Distant Site Mutations in Clinical TEM Beta-Lactamase Variants Enhance Non-Covalent Binding to Ceftazidime: Insights from Spectroscopic and Biophysical Investigations.

{beta}-lactamases retain the central armamentarium against the {beta}-lactams, resulting in surge of antibiotic resistance, primarily due to the hydrolysis of the amide bond of the four-membered ring. This study aimed to investigate the binding interactions of ceftazidime (CAZ) to TEM {beta}- lactamase variants with distant site mutations isolated from clinical setting to explore the cause of their selection and dissemination due to indiscriminate use of {beta}-lactams. Absorbance and fluorescence spectroscopy along with biophysical experimentation indicated facilitated binding of CAZ to the active site of the mutants than the wild type. The CAZ-TEM {beta}-lactamase mutant interactions were predominantly hydrophobic compared to H-bonding and van der Waals forces in the CAZ-wild type complex. Additionally structural alteration to justify more rigid binding of CAZ to the mutants in contrast to the wild type enzyme was established in silico. Therefore, acquisition of distant site mutations with respect to the active site of the {beta}-lactamase variants that rendered conformational flexibility to accommodate CAZ was evidenced. This study provided an insight to the bioactive interaction of CAZ with TEM {beta}-lactamase variants that probably facilitated their selection from clinical settings in response to rampant usage of {beta}-lactams.

microbiology↗

Trichomonas vaginalis extracellular vesicles suppress IFNϵ-mediated protection against host cell cytolysis

Trichomonas vaginalis is a commonly acquired sexually transmitted infection (STI) often found in symbiosis with the intracellular bacterium Mycoplasma hominis, an opportunistic pathogen of the female reproductive tract associated with bacterial vaginosis. How this symbiosis affects infection outcomes, and the host cell innate immune response is still poorly understood. Here we show that T. vaginalis extracellular vesicles down-regulate a non-canonical type I interferon, interferon-epsilon, and suppress type I interferon responses. Transcriptomic analysis reveals that infection with T. vaginalis in symbiosis with M. hominis or M. hominis alone upregulates genes involved in the type I IFN response, but infection with T. vaginalis alone does not. Finally, we show that interferon-epsilon stimulation is protective against T. vaginalis cytoadherence and cytolysis of host cells and increases the ability of neutrophils to kill the parasite. These studies provide insight into the innate immune response induced by a highly prevalent STI and its bacterial symbiont.

microbiology↗