bioRxiv Science⌕ Search

Biology subjects

Morris, R. K.

Publications and source records attributed to Morris, R. K..

2 recordsLinked to original sources

Effects of a temperate heatwave on diel rhythms of insect activity: a comparison across habitats

O_LIThe increasing frequency and intensity of heatwave events in temperate climates threatens to alter behavioural rhythms of ectothermic animals, such as insects. However, it is poorly understood how heatwaves affect daily activity patterns of insects, and whether shaded microclimates can moderate these responses. C_LIO_LIWe investigated impacts of a heatwave on the diel profile of insect activity, comparing effects across open, tree-covered and hedged habitats. Using yellow pan traps, insect activity was monitored from 07:00 to 19:00 on ten non-consecutive days, including two during a heatwave. C_LIO_LIInsect counts exhibited a unimodal relationship with temperature. C_LIO_LIDuring heatwave days, open habitat exhibited a significant ([~]81.9%) reduction in counts compared to two non-heatwave field-days, one before and one after the heatwave. Smaller, non-significant reductions were observed in the tree-covered (38.3%) and hedged (17.8%) habitats. C_LIO_LIThe diel activity profile on non-heatwave days approximated to a unimodal relationship, with GLMM-estimated counts peaking around 15:00; by contrast, heatwave days exhibited a bimodal profile, with predicted counts highest in the morning and evening. C_LIO_LISuch heatwave-induced deformations of activity patterns, modelled as interactions between heatwave and time-of-day, were significant across all three habitat types. C_LIO_LIThe findings suggest that temperate heatwaves can markedly decrease insect activity levels, and that whilst shade-providing vegetational features may reduce this effect, diel patterns of activity are affected landscape-wide. As heatwaves become more frequent, preservation of trees and hedges in temperate landscapes is likely crucial to support resilience of insect activity and wider ecosystem functioning. C_LI

ecology↗

Prolonged signaling of backbone-modified glucagon-like peptide-1 analogues with diverse receptor trafficking

Signal duration and subcellular location are emerging as important facets of G protein-coupled receptor (GPCR) function. The glucagon-like peptide-1 receptor (GLP-1R), a clinically relevant class B1 GPCR, stimulates production of the second messenger cAMP upon activation by the native hormone, GLP-1. cAMP production continues after the hormone-receptor complex has been internalized via endocytosis. Here, we report GLP-1 analogues that induce prolonged signaling relative to GLP-1. A single {beta}-amino acid substitution at position 18, with the residue derived from (S,S)-trans-2-aminocyclopentanecarboxylic acid (ACPC), enhances signaling duration with retention of receptor endocytosis. Pairing ACPC at position 18 with a second substitution, -aminoisobutyric acid (Aib) at position 16, abrogates endocytosis, but prolonged signaling is maintained. Prolonged signaling is sensitive to the structure of the {beta} residue at position 18. Cryo-electron microscopy (cryo-EM) structures of two GLP-1 analogues bound to the GLP- 1R:Gs complex suggest substantial alterations to bound peptide structure and dynamics compared to the GLP-1:GLP-1R complex. These structural findings strengthen an emerging view that agonist dynamics in the receptor-bound state influence signaling profile. Our results advance understanding of the structural underpinnings of receptor activation and introduce new tools for exploring the impact of spatiotemporal signaling profiles following GLP-1R activation.

pharmacology and toxicology↗