Biology subjects

Moerkerke, B.

Publications and source records attributed to Moerkerke, B..

4 recordsLinked to original sources

bioRxiv2019

Standardized Effect Sizes and Image-Based Meta-Analytical Approaches for fMRI Data.

Scientific progress is based on the ability to compare opposing theories and thereby develop consensus among existing hypotheses or create new ones. We argue that data aggregation (i.e. combine data across studies or research groups) for neuroscience is an important tool in this process. An important prerequisite is the ability to directly compare fMRI results over studies. In this paper, we discuss how an observed effect size in an fMRI data-analysis can be transformed into a standardized effect size. We demonstrate how these enable direct comparison and data aggregation over studies. Furthermore, we also discuss the influence of key parameters in the design of an fMRI experiment (such as number of scans and the sample size) on (statistical) properties of standardized effect sizes. In the second part of the paper, we give an overview of two approaches to aggregate fMRI results over studies. The first corresponds to extending the two-level general linear model approach as is typically used in individual fMRI studies with a third level. This requires the parameter estimates corresponding to the group models from each study together with estimated variances and meta-data. Unfortunately, there is a risk of running into unit mismatches when the primary studies use different scales to measure the BOLD response. To circumvent, it is possible to aggregate (unitless) standardized effect sizes which can be derived from summary statistics. We discuss a general model to aggregate these and different approaches to deal with between-study heterogeneity. Furthermore, we hope to further promote the usage of standardized effect sizes in fMRI research.

neuroscience↗

bioRxiv2019

Including Data Analytical Stability in Cluster-basedInference

In the statistical analysis of functional Magnetic Resonance Imaging (fMRI) brain data it remains a challenge to account for simultaneously testing activation in over 100.000 volume units or voxels. A popular method that reduces the dimensionality of this test problem is cluster-based inference. We propose a new testing procedure that allows to control the family-wise error (FWE) rate at the cluster level but improves cluster-based test decisions in two ways by (1) taking into account a measure for data analytical stability and (2) allowing a more voxel-based interpretation of the results. For each voxel, we define the re-selection rate conditional on a given FWE-corrected threshold and use this rate, which is a measure of stability, into the selection process. In our procedure, we set a more liberal and a more conservative FWE controlling threshold. Clusters that survive the liberal but not the conservative threshold are retained if sufficient evidence for voxelwise stability is available. Cluster that survive the conservative threshold are retained anyhow, and clusters that do not survive the liberal threshold are not further considered. Using the Human Connectome Project Data (Van Essen et al., 2012), we demonstrate how in a group analysis our method results not only in a higher number of selected voxels but also in a larger overlap between different test images. Additionally, we demonstrate the ability of our procedure to control the FWE, also in relatively small sample sizes.

neuroscience↗

bioRxiv2017

Assessing robustness against potential publication bias in coordinate based fMRI meta-analyses using the Fail-Safe N

The importance of integrating research findings is incontrovertible and coordinate based meta-analyses have become a popular approach to combine results of fMRI studies when only peaks of activation are reported. Similar to classical meta-analyses, coordinate based meta-analyses may be subject to different forms of publication bias which impacts results and possibly invalidates findings. We develop a tool that assesses the robustness to potential publication bias on cluster level. We investigate the possible influence of the file-drawer effect, where studies that do not report certain results fail to get published, by determining the number of noise studies that can be added to an existing fMRI meta-analysis before the results are no longer statistically significant. In this paper we illustrate this tool through an example and test the effect of several parameters through extensive simulations. We provide an algorithm for which code is freely available to generate noise studies and enables users to determine the robustness of meta-analytical results.

neuroscience↗

bioRxiv2017

The Influence Of Study Characteristics On Coordinate-Based fMRI Meta-Analyses

Given the increasing amount of neuroimaging studies, there is a growing need to summarize published results. Coordinate-based meta-analyses use the locations of statistically significant local maxima with possibly the associated effect sizes to aggregate studies. In this paper, we investigate the influence of key characteristics of a coordinate-based meta-analysis on (1) the balance between false and true positives and (2) the reliability of the outcome from a coordinate-based meta-analysis. More particularly, we consider the influence of the chosen group level model at the study level (fixed effects, ordinary least squares or mixed effects models), the type of coordinate-based meta-analysis (Activation Likelihood Estimation, fixed effects and random effects meta-analysis) and the amount of studies included in the analysis (10, 20 or 35). To do this, we apply a resampling scheme on a large dataset (N = 1400) to create a test condition and compare this with an independent evaluation condition. The test condition corresponds to subsampling participants into studies and combine these using meta-analyses. The evaluation condition corresponds to a high-powered group analysis. We observe the best performance when using mixed effects models in individual studies combined with a random effects meta-analysis. This effect increases with the number of studies included in the meta-analysis. We also show that the popular Activation Likelihood Estimation procedure is a valid alternative, though the results depend on the chosen threshold for significance. Furthermore, this method requires at least 20 to 35 studies. Finally, we discuss the differences, interpretations and limitations of our results.

neuroscience↗