Biology subjects

Mestre-Frances, N.

Publications and source records attributed to Mestre-Frances, N..

2 recordsLinked to original sources

bioRxiv2025

sKL/mKL Transcript Ratio and Protein Localization Define a Species- and Region-Specific Klotho Signature in the CNS and AD Progression

-Klotho is a multifunctional protein widely recognized for its anti-aging and neuroprotective properties. This study investigates the expression and localization of the secreted Klotho (s-KL) isoform in the human brain and its potential role in Alzheimers disease. Using RT-qPCR, we observed that the s-KL transcript predominates over the membrane-bound KL (m-KL) in multiple brain regions, a pattern consistent in macaques and lemurs. Immunohistochemistry and immunoprecipitation assays confirmed the presence of the s-KL protein in human and mouse brain parenchyma, revealing species-specific cellular localization. In human cerebrospinal fluid (CSF), s-KL constitutes [~]28% of total KL, with levels significantly reduced in mild dementia-AD patients. These findings underscore s-KLs potential neuroprotective role and highlight its differential regulation and expression during AD progression.

neuroscience↗

bioRxiv2019

Identification of cerebellar afferent projections in a nonhuman primate using CAV-2 vectors

Until relatively recently, the cerebellum was considered primarily as modulator of fine motor functions. This has changed during the last 20 years, and now the cerebellum has been shown to be involved in learning and cognition. With this renewed interest comes the need to better understand and potentially modify its input-output connections. In this pilot study, we tested the efficacy of a canine adenovirus type 2 (CAV-2) vector in the cerebellum of a nonhuman primate (NHP). Consistent with other reports, we found a preferentially transduction of cells with neuron-like morphology at the site of injection and efficient retrograde transport into several structures in the midbrain. These data will help identify cerebellar circuits and may lay the foundation for studies of human pathologies, such as ataxias, autism, and schizophrenia.

neuroscience↗