Biology subjects

Melano, R. G.

Publications and source records attributed to Melano, R. G..

3 recordsLinked to original sources

bioRxiv2024

EMERGENCE OF OXA-48-LIKE CARBAPENEMASES IN CLINICAL ENTEROBACTERALES FROM GUATEMALA

Five isolates (four Klebsiella pneumoniae and one Escherichia coli) displaying resistance to piperacillin/tazobactam and ertapenem and reduced susceptibility to imipenem were found positive for blaOXA-48-like genes (blaOXA-181 and blaOXA-232) carried on plasmids described previously. Although two K. pneumoniae recovered in 2020 were clonally related, the results suggest that blaOXA-48-like genes have been circulating in Guatemala since 2019. This is the first report of this type of carbapenemase in Central America.

microbiology↗

bioRxiv2021

Performance characteristics of next-generation sequencing for antimicrobial resistance gene detection in genomes and metagenomes

Short-read sequencing provides a culture-independent method for the detection of antimicrobial resistance (AMR) genes from single bacterial genomes and metagenomic samples. However, the performance characteristics of these approaches have not been systematically characterized. We compared assembly- and read-based approaches to determine sensitivity, positive predictive value, and sequencing limits of detection required for AMR gene detection in an Escherichia coli ST38 isolate spiked into a synthetic microbial community at varying abundances. Using an assembly-based method the limit of detection was 15X genome coverage. We are confident in AMR gene detection at target relative abundances of 100% to 1%, where a target abundance of 1% would require assembly of approximately 30 million reads to achieve 15X target coverage. Recent studies assessing AMR gene content in metagenomic samples may be inadequately sequenced to achieve high sensitivity. Our study informs future sequencing projects and analytical strategies for genomic and metagenomic AMR gene detection.

bioinformatics↗

bioRxiv2018

Diverse Escherichia coli lineages, from domestic animals and humans in a household, carry colistin resistance gene mcr-1 in Ecuador

The aim of this study was to investigate the presence of Escherichia coli carrying mcr-1 gene in domestic animals close to a child who suffered a peritoneal infection by a mcr-1 positive E. coli. Rectal or cloacal swabs and fecal samples from domestic animals were plated on selective media to isolate colistin-resistant E. coli and isolates were submitted to detection of mcr-1 gene, pulsed field gel electrophoresis (PFGE), multi-locus sequence typing (MLST), replicon typing and S1-PFGE. Four mcr-1 positive E. coli isolates (from chicken, turkey and dog) were recovered. No shared PFGE pattern or MLST sequence type were observed among isolates. A 60Kb IncI1{gamma} mcr-1-carrying plasmid was detected in all isolates. Our results suggest that mcr-1 gene was horizontally disseminated amongst different lineages of E. coli from domestic animals in the childs household.\n\nImportanceHorizontally transferable colistin resistance (mcr-1 gene) is thought to have originated in domestic animals and transferred to humans through meat and dairy products. In the present report we show evidence that the mcr-1 gene could be transferred to different E. coli strains colonizing different hosts (humans and pets) in the same household.

microbiology↗