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Megges, M.

Publications and source records attributed to Megges, M..

2 recordsLinked to original sources

RNA-sequencing reveals strong predominance of THRA splicing isoform 2 in the developing and adult human brain

Thyroid hormone receptor alpha (THR) is a nuclear hormone receptor that binds triiodothyronine (T3) and acts as an important transcription factor in development, metabolism and reproduction. THR has in mammals two major splicing isoforms, THR1 and THR2. The better characterized isoform, THR1, is a transcriptional stimulator of genes involved in cell metabolism and growth. The less well characterized isoform, THR2, lacks the Ligand Binding Domain (LBD) and is thought to act as an inhibitor of THR1 action. The ratio of THR1 to THR2 splicing isoforms is therefore critical for transcriptional regulation in different tissues and during development. However, the expression patterns of both isoforms have not been studied in healthy human tissues or in the developing brain. Given the lack of commercially available isoform-specific antibodies, we addressed this question by analyzing four bulk RNA-sequencing datasets and two scRNA-sequencing datasets to determine the RNA expression levels of human THRA1 and THRA2 transcripts in healthy adult tissues and in the developing brain. We demonstrate how 10X Chromium scRNA-seq datasets can be used to perform splicing-sensitive analyses of isoforms that differ at the 3-end. In all datasets, we discovered a strong predominance of THRA2 transcripts at all investigated stages of human brain development and in the central nervous system from healthy human adults.

genetics↗

Spatiotemporal expression of thyroid hormone transporter MCT8 and THRA mRNA in human cerebral organoids recapitulating first trimester cortex development

Thyroid hormones (TH) play critical roles during nervous system development and patients carrying coding variants of MCT8 (Monocarboxylate transporter 8) or THRA (Thyroid hormone receptor alpha) present a spectrum of neurological phenotypes resulting from perturbed local TH action during early brain development. Recently, human cerebral organoids (hCOs) emerged as powerful three-dimensional in vitro tools for disease modelling recapitulating key aspects of early human cerebral cortex development. To begin exploring prospects of this model for thyroid research, we performed a detailed characterization of the spatiotemporal expression of the TH transporter MCT8 and TH receptor THRA in developing hCOs. Immunostaining of hCOs showed MCT8 membrane expression in neuronal progenitor cell types including early neuroepithelial cells, SOX2+/Nestin+ radial glia cells (RGCs), TBR2+ intermediate progenitors and HOPX+ outer RGCs. In addition, we detected robust MCT8 protein expression in CTIP2+ deep layer neurons and at later developmental stages in SATB2+ upper layer neurons. Spatiotemporal SLC16A2 mRNA expression, detected by fluorescent in situ hybridization (FISH), was highly concordant with MCT8 protein expression across cortical cell layers. FISH detected THRA mRNA expression already in neuroepithelium before the onset of neurogenesis and THRA expression was maintained in RGCs of the ventricular zone. Increased THRA expression was later detected in the subventricular zone whereas highest THRA expression was observed in excitatory neurons in peripheral hCO regions. In combination with strong up-regulation of known T3 response genes following short-term T3 treatment of hCOs, these observations show that hCOs provide a promising and experimentally tractable model to probe local TH action during human cortical neurogenesis and eventually to model the consequences of impaired TH function for early cortex development.

neuroscience↗