Biology subjects

McAdam, S.

Publications and source records attributed to McAdam, S..

8 recordsLinked to original sources

bioRxiv2026

The origin of mechanical advantage in angiosperms

O_LIMechanical interaction between guard cells and epidermal pavement cells enables large stomatal apertures and high productivity in angiosperms. We do not know when this response evolved, but over the last 169 years we have found that mechanical advantage has been tested in at least 230 species from 85 families. To date no data on this trait exists among angiosperms outside magnoliids, monocots and eudicots. C_LIO_LITo resolve the evolutionary origins of this critical stomatal response we tested for mechanical advantage across 14 additional species including the earliest diverging lineages of angiosperms. C_LIO_LIWe find that mechanical advantage, while variable in magnitude, is present in all angiosperm species that have been measured, including Amborella trichopoda sister to all angiosperms. C_LIO_LIThis response likely evolved once in flowering plants, in the common ancestor of this clade, remaining widespread across angiosperms today. We hypothesize that angiosperms could not have realized the full potential of physiological innovations in water transport without the evolution of this key trait that increased operational stomatal aperture. C_LI

plant biology↗

bioRxiv2025

Making a clean break: contrasting leaf abscission dynamics across temperate leaf habits

Background and AimsLeaf senescence allows plants to reallocate nutrients from aging leaves, often storing them for future use. As chlorophyll breaks down, other pigments emerge, creating the vibrant colors of autumn. In many deciduous trees, senescence is followed by abscission the physical detachment of leaves at the abscission zone (AZ). Though closely linked, senescence and abscission are distinct; some plants senesce without abscising, while others abscise partially senesced leaves. Most research on these processes has focused on herbaceous and crop species, which do not naturally shed leaves. MethodsTo better understand how leaf senescence and abscission occur in temperate deciduous trees, we developed a novel method to quantify AZ competency (AZC) we also measured gas exchange, chlorophyll content, water potential, and abscisic acid in four tree species representing deciduous, brevi-deciduous, and marcescent leaf habits. Key resultsThe two deciduous species showed contrasting patterns: one degraded chlorophyll and ceased photosynthesis before AZC developed; the other retained chlorophyll and continued photosynthesis until nearly all AZs became competent. The brevi-deciduous species lost most chlorophyll but developed AZs gradually over a longer period. The marcescent species fully senesced but did not develop AZC. ConclusionsThese findings show that senescence and abscission are distinct and variably timed processes across temperate tree species.

plant biology↗

bioRxiv2025

Identification of QTLs linked to partial resistance to foot and root rot caused by Fusarium avenaceum and Fusarium oxysporum in faba bean (Vicia faba)

Foot and root rot, caused by a complex of soil-borne fungal and oomycete pathogens, including several Fusarium spp., can cause serious yield losses in faba bean. Current control strategies rely largely on agronomic practices and limited varietal resistance. Identifying novel sources of genetic resistance is of great potential value for breeding elite varieties with improved foot rot resistance. A partially resistant Vicia faba accession ig124213 (NV490) was crossed with a moderately susceptible ig124301 (NV512) and a mapping population comprising 198 F3 families was developed. This was screened for resistance to a mixture of seven UK field isolates of Fusarium avenaceum and Fusarium oxysporum under glasshouse conditions. Several F3 families with moderate to high levels of resistance to both Fusarium species were identified, and a high-density linkage map of the V. faba genome including 6755 SNP-markers in seven linkage groups (LG) was generated using the Vfaba_v2 Axiom SNP array. Quantitative trait loci associated with improved resistance to Fusarium foot and root rot were identified, including one major QTL on LG4, corresponding to the chromosome 4 of V. faba. Key messageThe first QTL associated with partial resistance to Fusarium foot and root rot has been identified using a biparental mapping population of Vicia faba.

plant biology↗

bioRxiv2023

Wind speed affects the rate and kinetics of stomatal conductance

Understanding the relationship between wind speed and gas exchange in plants is a longstanding challenge. Our aim was to investigate the impact of wind speed on maximum rates of gas exchange and the kinetics of stomatal responses. We conducted experiments using an infrared gas analyzer equipped with a controlled leaf fan, enabling precise control of the boundary layer conductance. We first showed that the chamber was adequately mixed even at extremely low fan speeds (down to 200 rpm, equivalent to a wind speed of 0.0005 m s-1) and evaluated the link between fan speed, wind speed, and boundary layer conductance. We observed that higher wind speeds led to increased gas exchange of both water vapor and CO2 in Arabidopsis, presumably due to its effect on transpiration and the consequential reduction in epidermal pressure that led to stomatal opening. We documented that stomatal opening in response to light was three times faster at a fan speed of 10000 rpm (wind speed of 2 m s-1) compared with 500 rpm (0.25 m s-1) in Vicia faba, while the latter exhibited an opening rate that was similar to those of epidermal peels. The increase of stomatal conductance under high wind was observed in four species under field conditions. Our findings demonstrate the importance of the size of the boundary layer on determining maximum rates of gas exchange and the kinetics of gas exchange responses to environmental changes.

plant biology↗

bioRxiv2023

Passive stomatal closure under extreme drought in an angiosperm species

The phytohormone abscisic acid (ABA), synthesized as leaf turgor declines, plays a major role in closing stomata in species from this lineage, but recent reports of some angiosperms having a peaking-type ABA dynamic in which under extreme drought ABA levels decline to pre-stressed levels raises the possibility that passive stomatal closure by leaf water status alone can occur in species from this lineage. To test this hypothesis, we conducted instantaneous rehydration experiments in the peaking-type species Umbellularia californica through a long-term drought in which ABA levels declined to pre-stress levels yet stomata remain closed. We found that when ABA levels were lowest during extreme drought stomata of U. californica were passively closed by leaf water status alone, with stomata reopening rapidly to maximum rates of gas exchange on instantaneous rehydration. This contrasts with leaves early in drought in which ABA levels are highest, where we found stomata do not reopen on instantaneous rehydration. The transition from ABA driven stomatal closure to passively driven stomatal closure as drought progresses in this species occurs at very low water potentials facilitated by highly embolism resistant xylem. These results have important implications for understanding stomatal control during drought in angiosperms.

plant biology↗

bioRxiv2023

Stomatal dynamics are regulated by leaf hydraulic traits and guard cell anatomy in nine true mangrove species

Stomatal regulation is critical for mangroves to survive water deficits and highly fluctuating ambient water availability in the hyper-saline intertidal zone. Despite the importance of stomatal regulation in mangroves very little is known about stomatal sensitivity to vapour pressure deficit (VPD), and the co-ordination of this trait with stomatal morphology and leaf hydraulic traits in these species. We measured the stomatal response to a step increase in vapour pressure deficit (VPD) in situ, stomatal anatomy, leaf hydraulic vulnerability and pressure-volume traits in nine true mangrove species of five families. We aimed to answer two questions: (1) Does stomatal morphology determine stomatal dynamics in response to a high VPD in mangroves and (2) do leaf hydraulic traits influence stomatal sensitivity to VPD in mangroves? We found that the stomata of mangrove plants highly sensitive to VPD, and that species with higher maximum stomatal conductance had slower stomatal responses to an increase in VPD, and that stomatal density and size were correlated with the speed of stomatal closure at high VPD across the closely-related species. We also found that a higher leaf capacitance (Cleaf) and more resistance to leaf hydraulic vulnerability were associated with slower stomatal responses to an increase in VPD. Our results demonstrate that the dynamics of the stomatal response to an increase in VPD are regulated by leaf hydraulic traits and stomatal morphology. Our work provides a quantitative framework to better understand stomatal regulation in mangroves in an environment with highly dynamic water availability.

ecology↗

bioRxiv2021

THESEUS1 modulates cell wall stiffness and abscisic acid production in Arabidopsis thaliana

Plant cells can be distinguished from animal cells by their cell walls and high turgor pressure. Although changes in turgor and stiffness of cell walls seem coordinated, we know little about the mechanism responsible for coordination. Evidence has accumulated that plants, like yeast, have a dedicated cell wall integrity maintenance mechanism. This mechanism monitors the functional integrity of the wall and maintains it through adaptive responses when cell wall damage occurs during growth, development, and interactions with the environment. The adaptive responses include osmo-sensitive-induction of phytohormone production, defence responses as well as changes in cell wall composition and structure. Here, we investigate how the cell wall integrity maintenance mechanism coordinates changes in cell wall stiffness and turgor in Arabidopsis thaliana. We show that the production of abscisic acid (ABA), the phytohormone modulating turgor pressure and responses to drought, depends on the presence of a functional cell wall. We find that the cell wall integrity sensor THESEUS1 modulates mechanical properties of walls, turgor loss point and ABA biosynthesis. We identify RECEPTOR-LIKE PROTEIN 12 as a new component of cell wall integrity maintenance controlling cell wall damage-induced jasmonic acid production. Based on the results we propose that THE1 is responsible for coordinating changes in turgor pressure and cell wall stiffness. Significance statementPlants need to constantly adapt to a changing environment. This includes responses to biotic and abiotic stress. Key elements influencing the response to abiotic stress are the plant cell walls surrounding all cells and the phytohormone abscisic acid, which influences turgor pressure in plants. Turgor pressure in plant cells is much higher than in animal cells and a key driver of plant growth and development. Here we investigate the mechanism regulating cell wall stiffness and coordinating changes in stiffness and turgor. We characterize key elements of the mechanism and dissect its mode of action. This knowledge will enable us to pursue novel approaches to improve plant resistance to drought stress, which is crucial in a rapidly changing environment.

plant biology↗

bioRxiv2021

Timing of meristem initiation and maintenance determines the morphology of fern gametophytes

The alternation of generations in land plants occurs between the sporophyte phase and the gametophyte phase. The sporophytes of seed plants develop self-maintained, multicellular meristems, and these meristems determine plant architecture. The gametophytes of seed plants lack meristems and are heterotrophic. In contrast, the gametophytes of seed-free vascular plants, including ferns, are autotrophic and free-living, developing meristems to sustain their independent growth and proliferation. Compared to meristems in the sporophytes of seed plants, the cellular mechanisms underlying meristem development in fern gametophytes remain largely unknown. Here, using confocal time-lapse live imaging and computational segmentation and quantification, we determined different patterns of cell divisions associated with the initiation and proliferation of two distinct types of meristems in fern gametophytes. Our results reveal how the simple timing of a switch between two meristems has considerable consequences for the divergent gametophyte morphologies of two closely related ferns from Pteridaceae (Pteris and Ceratopteris). Our result provides evolutionary insight into the function and regulation of gametophyte meristems in seed-free vascular plants. HighlightLive-imaging of cell growth and division in apical initials and lateral meristems reveals that the timing of a switch between the two meristem identities drives morphology variation in fern gametophytes.

plant biology↗